Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on Form S-3of Brooks Automation, Inc.of our report dated November 29, 2018relating to the financial statements and the effectiveness of internal control over financial reporting, which appears in Brooks Automation, Inc.'s Annual Report on Form 10-K for the year ended September 30, 2018.  We also consent to the reference to us under the heading “Experts” in such Registration Statement.

/s/ PricewaterhouseCoopers LLP
Boston, MassachusettsAugust 8, 2019