-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
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 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 JBiBG1ayw3ChJnPCxLjE/Hp+7A2/lYOK46wEPIm+ZtqElnhCCzb2XySpzxOKhcPw
 R5VOD7yfiF7hnxlvLVhoeg==

<SEC-DOCUMENT>0000926617-10-000003.txt : 20100112
<SEC-HEADER>0000926617-10-000003.hdr.sgml : 20100112
<ACCEPTANCE-DATETIME>20100111195658
ACCESSION NUMBER:		0000926617-10-000003
CONFORMED SUBMISSION TYPE:	D
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20100112
DATE AS OF CHANGE:		20100111
EFFECTIVENESS DATE:		20100112

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			VERMILLION, INC.
		CENTRAL INDEX KEY:			0000926617
		STANDARD INDUSTRIAL CLASSIFICATION:	LABORATORY ANALYTICAL INSTRUMENTS [3826]
		IRS NUMBER:				330595156
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-92661-7L
		FILM NUMBER:		10521343

	BUSINESS ADDRESS:	
		STREET 1:		47350 FREMONT BLVD.
		CITY:			FREMONT
		STATE:			CA
		ZIP:			94538
		BUSINESS PHONE:		510-226-2800

	MAIL ADDRESS:	
		STREET 1:		47350 FREMONT BLVD.
		CITY:			FREMONT
		STATE:			CA
		ZIP:			94538

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	CIPHERGEN BIOSYSTEMS INC
		DATE OF NAME CHANGE:	20000316

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	ABIOTIC SYSTEMS
		DATE OF NAME CHANGE:	19950407
</SEC-HEADER>
<DOCUMENT>
<TYPE>D
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0000926617</cik>
        <entityName>VERMILLION, INC.</entityName>
        <issuerAddress>
            <street1>47350 FREMONT BLVD.</street1>
            <city>FREMONT</city>
            <stateOrCountry>CA</stateOrCountry>
            <zipCode>94538</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>510-226-2800</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>CIPHERGEN BIOSYSTEMS INC</previousName>
            <previousName>ABIOTIC SYSTEMS</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gail</firstName>
                <middleName>S.</middleName>
                <lastName>Page</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>47350 Fremont Blvd.</street1>
                <city>Fremont</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94538</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Executive Chair of the Board of Directors</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John</firstName>
                <middleName>F.</middleName>
                <lastName>Hamilton</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>47350 Fremont Blvd.</street1>
                <city>Fremont</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94538</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>James</firstName>
                <middleName>S.</middleName>
                <lastName>Burns</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>47350 Fremont Blvd.</street1>
                <city>Fremont</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94538</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Health Care</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2010-01-07</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>99993</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>43049858</totalOfferingAmount>
            <totalAmountSold>43049858</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>14</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>The Issuer paid an aggregate of $100,000 in transaction advisory fees in connection with the sale of securities reported on this Form D.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>No proceeds are proposed to be used for payments to such persons other than ordinary salary expenses that may be paid out of working capital.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>VERMILLION, INC.</issuerName>
                <signatureName>Gail S. Page</signatureName>
                <nameOfSigner>Gail S. Page</nameOfSigner>
                <signatureTitle>Executive Chair of the Board of Directors</signatureTitle>
                <signatureDate>2010-01-08</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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