Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form S-1 (Nos. 333-169755, 333-177244, 333-184944 and 333-189823) and the Registration Statements on Form S-8 (Nos. 333-63758, 333-81326, 333-128082, 333-178261 and 333-183312) of Guided Therapeutics, Inc. and Subsidiary of our report dated March 26, 2014, relating to the consolidated financial statements, which appears in this Form 10-K for the year ended December 31, 2013.

/s/ UHY LLP

UHY LLP

Sterling Heights, Michigan

March 26, 2014