-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
 MFgwCgYEVQgBAQICAf8DSgAwRwJAW2sNKK9AVtBzYZmr6aGjlWyK3XmZv3dTINen
 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 T9Pwc3NkfYRvNCamDag93T9TKUYn0IW49eGhhKduqJtMfTFqXVDHXOvArEAbyjst
 PZ39oKpD5dFMQX44Ykiktg==

<SEC-DOCUMENT>0001209191-05-025093.txt : 20050510
<SEC-HEADER>0001209191-05-025093.hdr.sgml : 20050510
<ACCEPTANCE-DATETIME>20050510165437
ACCESSION NUMBER:		0001209191-05-025093
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20050506
FILED AS OF DATE:		20050510
DATE AS OF CHANGE:		20050510

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			TWADDELL SOPHIA H
		CENTRAL INDEX KEY:			0001209296

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	000-24050
		FILM NUMBER:		05817533

	MAIL ADDRESS:	
		STREET 1:		1560 SHERMAN AVENUE
		STREET 2:		SUITE 1000
		CITY:			EVANSTON
		STATE:			IL
		ZIP:			60201

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			NORTHFIELD LABORATORIES INC /DE/
		CENTRAL INDEX KEY:			0000920947
		STANDARD INDUSTRIAL CLASSIFICATION:	BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
		IRS NUMBER:				363378733
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			0531

	BUSINESS ADDRESS:	
		STREET 1:		1560 SHERMAN AVE
		STREET 2:		SUITE 1000
		CITY:			EVANSTON
		STATE:			IL
		ZIP:			60201-4800
		BUSINESS PHONE:		8478643500

	MAIL ADDRESS:	
		STREET 1:		1560 SHERMAN AVE STE 1000
		STREET 2:		37TH FLOOR
		CITY:			EVANSTON
		STATE:			IL
		ZIP:			60201-4800
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>boc95167_01st.xml
<DESCRIPTION>MAIN DOCUMENT DESCRIPTION
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0202</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2005-05-06</periodOfReport>

    <issuer>
        <issuerCik>0000920947</issuerCik>
        <issuerName>NORTHFIELD LABORATORIES INC /DE/</issuerName>
        <issuerTradingSymbol>NFLD</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001209296</rptOwnerCik>
            <rptOwnerName>TWADDELL SOPHIA H</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>1560 SHERMAN AVE.</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 1000</rptOwnerStreet2>
            <rptOwnerCity>EVANSTON</rptOwnerCity>
            <rptOwnerState>IL</rptOwnerState>
            <rptOwnerZipCode>60201</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Vice President</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2005-05-06</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>750</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>14.88</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>2750</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable></derivativeTable>

    <ownerSignature>
        <signatureName>/s/ Sophia Twaddell</signatureName>
        <signatureDate>2005-05-10</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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