Exhibit 22.1

Subsidiary Guarantors

As of December 31, 2023, each of the following subsidiaries of Enbridge Inc. (“Enbridge”), both of which are indirect, wholly-owned subsidiaries of Enbridge, has fully and unconditionally guaranteed on an unsecured, joint and several basis, each of the registered debt securities of the Company listed below:

Subsidiary Guarantors

1.Spectra Energy Partners, LP, a Delaware limited partnership

2.Enbridge Energy Partners, L.P., a Delaware limited partnership

Registered Debt Securities of Enbridge Guaranteed by each of the Subsidiary Guarantors

1.Floating Rate Senior Notes due 2024

2.3.500% Senior Notes due 2024

3.2.150% Senior Notes due 2024

4.2.500% Senior Notes due 2025

5.2.500% Senior Notes due 2025

6.4.250% Senior Notes due 2026

7.1.600% Senior Notes due 2026

8.5.969% Senior Notes due 2026

9.5.900% Senior Notes due 2026

10.3.700% Senior Notes due 2027

11.6.000% Senior Notes due 2028

12.3.125% Senior Notes due 2029

13.6.200% Senior Notes due 2030

14.2.500% Sustainability-Linked Senior Notes due 2033

15.5.700% Sustainability-Linked Senior Notes due 2033

16.4.500% Senior Notes due 2044

17.5.500% Senior Notes due 2046

18.4.000% Senior Notes due 2049

19.3.400% Senior Notes due 2051

20.6.700% Senior Notes due 2053