EXHIBIT 21.1

A. & R. Line, Inc.

Alabama & Gulf Coast Railway LLC

Arizona & California Railroad Company

Bauxite & Northern Railway Company

California Northern Railroad Company

California Western Railroad, Inc.

Cape Breton & Central Nova Scotia Railway Limited

Cascade and Columbia River Railroad Company

Central Oregon & Pacific Railroad, Inc.

The Central Railroad Company of Indiana

Central Railroad Company of Indianapolis

Connecticut Southern Railroad, Inc.

Dakota Rail, Inc.

Dallas, Garland & Northeastern Railroad, Inc.

Dallas, Garland & Northeastern Railroad, Inc. (inactive)

Eastern Alabama Railway, LLC

Goderich-Exeter Railway Company Limited

Huron and Eastern Railway Company, Inc.

Indiana & Ohio Rail Corp.

Indiana & Ohio Railway Company

Indiana Southern Railroad, LLC

J.K. Line, Inc.

Kiamichi Railroad Company, L.L.C.

Kyle Railroad Company

Kyle Railways, LLC

The Massena Terminal Railroad Company

Mid-Michigan Railroad, Inc.

Missouri & Northern Arkansas Railroad Company, Inc.

New England Central Railroad, Inc.

New StatesRail Holdings, LLC

North Carolina & Virginia Railroad Company, LLC

Otter Tail Valley Railroad Company, Inc.

Palm Beach Rail Holding, Inc.

Plainview Terminal Company

Point Comfort & Northern Railway Company

Puget Sound & Pacific Railroad

RAAI Delaware, LLC

Rail Line Holdings #1, Inc.

RailAmerica Operations Shared Services, Inc., f/k/a American Rail Dispatching Center, Inc.

RailAmerica Operations Support Group, Inc. f/k/a Rail Operating Support Group, Inc.

RailAmerica Australia II, LLC

RailAmerica Contract Switching Services, Inc.

RailAmerica de Chile S.A.

RailAmerica Equipment Corp.

RailAmerica Intermodal Services, Inc.

RailAmerica Holding Services, Inc.

RailAmerica Transportation Corp.

RaiLink Acquisition, Inc.

RaiLink Canada Ltd.

RailTex Canada, Inc.

RailTex Distribution Services, Inc.

RailTex International Holdings, Inc.

RailTex, Inc.

RL Funding Corp.

Rockdale, Sandow & Southern Railroad Company

San Diego & Imperial Valley Railroad Company, Inc.

San Joaquin Valley Railroad Co.

San Pedro Trails, Inc.

South Carolina Central Railroad Company, LLC

South East Rail, Inc.

StatesRail II Railroad, LLC

StatesRail, LLC

SWKR Operating Co., Inc. d/b/a San Pedro & Southwestern Railway Company, Inc.

Toledo, Peoria & Western Railway Corp.

Trois-Rivières Trailers, Inc./Remorques Trois-Rivières Inc.

Ventura County Railroad Company

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