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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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<SEC-DOCUMENT>0001140361-10-025478.txt : 20100614
<SEC-HEADER>0001140361-10-025478.hdr.sgml : 20100614
<ACCEPTANCE-DATETIME>20100614194708
ACCESSION NUMBER:		0001140361-10-025478
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20100610
FILED AS OF DATE:		20100614
DATE AS OF CHANGE:		20100614

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			Roth Michael Anthony
		CENTRAL INDEX KEY:			0001010207

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-13835
		FILM NUMBER:		10896077

	MAIL ADDRESS:	
		STREET 1:		3600 SOUTH LAKE DRIVE
		CITY:			ST. FRANCIS
		STATE:			WI
		ZIP:			53235

	FORMER NAME:	
		FORMER CONFORMED NAME:	ROTH MICHAEL
		DATE OF NAME CHANGE:	19960307

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			APPLIED NEUROSOLUTIONS INC
		CENTRAL INDEX KEY:			0000872947
		STANDARD INDUSTRIAL CLASSIFICATION:	BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
		IRS NUMBER:				391661164
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	BUSINESS ADDRESS:	
		STREET 1:		50 LAKEVIEW PARKWAY S
		STREET 2:		STE 111
		CITY:			VERNON HILLS
		STATE:			IL
		ZIP:			60061
		BUSINESS PHONE:		847 573 8000

	MAIL ADDRESS:	
		STREET 1:		50 LAKEVIEW PARKWAY
		STREET 2:		STE 111
		CITY:			VERNON HILLS
		STATE:			IL
		ZIP:			60061

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	HEMOXYMED INC
		DATE OF NAME CHANGE:	20020820

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	OPHIDIAN PHARMACEUTICALS INC
		DATE OF NAME CHANGE:	19970714

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			STARK BRIAN JAY
		CENTRAL INDEX KEY:			0001010208

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-13835
		FILM NUMBER:		10896076

	MAIL ADDRESS:	
		STREET 1:		3600 SOUTH LAKE DRIVE
		CITY:			ST. FRANCIS
		STATE:			WI
		ZIP:			53235
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>doc1.xml
<DESCRIPTION>FORM 4
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0303</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2010-06-10</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0000872947</issuerCik>
        <issuerName>APPLIED NEUROSOLUTIONS INC</issuerName>
        <issuerTradingSymbol>APSN</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001010207</rptOwnerCik>
            <rptOwnerName>Roth Michael Anthony</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>3600 SOUTH LAKE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>ST. FRANCIS</rptOwnerCity>
            <rptOwnerState>WI</rptOwnerState>
            <rptOwnerZipCode>53235</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001010208</rptOwnerCik>
            <rptOwnerName>STARK BRIAN JAY</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>3600 SOUTH LAKE DR</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>ST FRANCIS</rptOwnerCity>
            <rptOwnerState>WI</rptOwnerState>
            <rptOwnerZipCode>53235</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, $0.0025 par value per share</value>
            </securityTitle>
            <transactionDate>
                <value>2010-06-10</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>510021</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0.04</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By SF Capital Partners Ltd.</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Warrants to purchase Common Stock, $0.0025 par value/share</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>5.700</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F2"/>
            </exerciseDate>
            <expirationDate>
                <value>2012-09-28</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock, $0.0025 par value per share</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>207142</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>207142</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By SF Capital Partners Ltd.</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The shares of Common Stock are held directly by SF Capital Partners Ltd. (&quot;SF Capital&quot;).  The Reporting Persons direct the management of Stark Offshore Management LLC (&quot;Stark Offshore&quot;), which acts as investment manager and has sole power to direct the management of SF Capital.  As the Managing Members of Stark Offshore, the Reporting Persons possess voting and dispositive power over all of the foregoing shares.  Therefore, for the purposes of Rule 13d-3 under the Securities Exchange Act of 1934, as amended, the Reporting Persons may be deemed to be the beneficial owners of, but hereby disclaim such beneficial ownership of, the foregoing shares.</footnote>
        <footnote id="F2">Immediately</footnote>
    </footnotes>

    <remarks></remarks>

    <ownerSignature>
        <signatureName>Michael A. Roth</signatureName>
        <signatureDate>2010-06-14</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Brian J. Stark</signatureName>
        <signatureDate>2010-06-14</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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