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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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<SEC-DOCUMENT>0001010207-09-000072.txt : 20090504
<SEC-HEADER>0001010207-09-000072.hdr.sgml : 20090504
<ACCEPTANCE-DATETIME>20090504164001
ACCESSION NUMBER:		0001010207-09-000072
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20090430
FILED AS OF DATE:		20090504
DATE AS OF CHANGE:		20090504

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			Roth Michael Anthony
		CENTRAL INDEX KEY:			0001010207

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-13835
		FILM NUMBER:		09793860

	MAIL ADDRESS:	
		STREET 1:		3600 SOUTH LAKE DRIVE
		CITY:			ST. FRANCIS
		STATE:			WI
		ZIP:			53235

	FORMER NAME:	
		FORMER CONFORMED NAME:	ROTH MICHAEL
		DATE OF NAME CHANGE:	19960307

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			APPLIED NEUROSOLUTIONS INC
		CENTRAL INDEX KEY:			0000872947
		STANDARD INDUSTRIAL CLASSIFICATION:	BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
		IRS NUMBER:				391661164
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	BUSINESS ADDRESS:	
		STREET 1:		50 LAKEVIEW PARKWAY S
		STREET 2:		STE 111
		CITY:			VERNON HILLS
		STATE:			IL
		ZIP:			60061
		BUSINESS PHONE:		847 573 8000

	MAIL ADDRESS:	
		STREET 1:		50 LAKEVIEW PARKWAY
		STREET 2:		STE 111
		CITY:			VERNON HILLS
		STATE:			IL
		ZIP:			60061

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	HEMOXYMED INC
		DATE OF NAME CHANGE:	20020820

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	OPHIDIAN PHARMACEUTICALS INC
		DATE OF NAME CHANGE:	19970714

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			STARK BRIAN JAY
		CENTRAL INDEX KEY:			0001010208

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-13835
		FILM NUMBER:		09793859

	MAIL ADDRESS:	
		STREET 1:		3600 SOUTH LAKE DRIVE
		CITY:			ST. FRANCIS
		STATE:			WI
		ZIP:			53235
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>form4apns20090504_ex.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0303</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2009-04-30</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0000872947</issuerCik>
        <issuerName>APPLIED NEUROSOLUTIONS INC</issuerName>
        <issuerTradingSymbol>APNS</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001010207</rptOwnerCik>
            <rptOwnerName>Roth Michael Anthony</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>3600 SOUTH LAKE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>ST. FRANCIS</rptOwnerCity>
            <rptOwnerState>WI</rptOwnerState>
            <rptOwnerZipCode>53235</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001010208</rptOwnerCik>
            <rptOwnerName>STARK BRIAN JAY</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>3600 SOUTH LAKE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>ST. FRANCIS</rptOwnerCity>
            <rptOwnerState>WI</rptOwnerState>
            <rptOwnerZipCode>53235</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, $0.0025 par value per share</value>
            </securityTitle>
            <transactionDate>
                <value>2009-04-30</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>100000</value>
                    <footnoteId id="F2"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0.02</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>23977022</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By: SF Capital Partners Ltd.</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, $0.0025 par value per share</value>
            </securityTitle>
            <transactionDate>
                <value>2009-05-01</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>28500</value>
                    <footnoteId id="F2"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0.02</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>23948522</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By: SF Capital Partners Ltd.</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The shares of Common Stock are held directly by SF Capital Partners Ltd. (&quot;SF Capital&quot;). The Reporting Persons direct the
 management of Stark Offshore Management LLC (&quot;Stark Offshore&quot;), which acts as investment manager and has sole power to
 direct the management of SF Capital. As the Managing Members of Stark Offshore, the Reporting Persons possess voting and
 dispositive power over all of the foregoing shares. Therefore, for the purposes of Rule 13d-3 under the Securities Exchange
 Act of 1934, as amended, the Reporting Persons may be deemed to be the beneficial owners of, but hereby disclaim such
 beneficial ownership of, the foregoing shares.</footnote>
        <footnote id="F2">This transaction was executed in multiple trades at a price of $0.02.  The price reported above reflects the actual sale
 price.  The reporting person hereby undertakes to provide upon request to the SEC staff, the issuer or a security holder of
 the issuer full information regarding the number of shares at which each trade was effected.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>Michael A. Roth</signatureName>
        <signatureDate>2009-05-04</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Brian J. Stark</signatureName>
        <signatureDate>2009-05-04</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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