10-Q

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM 10-Q

(Mark One)

☒ Quarterly Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the quarterly period ended June 30, 2021

or

☐ Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the transition period from _____________ to _____________

Commission File Number 001-38185

PRESSURE BIOSCIENCES, INC.

(Exact name of registrant as specified in its charter)

Massachusetts	04-2652826
(State or other jurisdiction of	(I.R.S. Employer
incorporation or organization)	Identification No.)

14 Norfolk Avenue
South Easton, Massachusetts	02375
(Address of principal executive offices)	(Zip Code)

(508) 230-1828

(Registrant’s telephone number, including area code)

Securities registered pursuant to Section 12(b) of the Act:

Title of each class	Trading Symbol(s)	Name of each exchange on which registered
N/A	N/A	N/A

Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been subject to such filing requirements for the past 90 days.

☒ Yes ☐ No

Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S-T (§232.405 of this chapter) during the preceding 12 months (or for such shorter period that the registrant was required to submit such files).

☒ Yes ☐ No

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting company. See the definitions of “large accelerated filer,” “accelerated filer,” “smaller reporting company,” and “emerging growth company” in Rule 12b-2 of the Exchange Act. (Check one):

Large accelerated filer	☐	Accelerated filer	☐
Non-accelerated Filer	☒	Smaller Reporting Company	☒
Emerging Growth Company	☐

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐

Indicate by check mark whether the registrant is a shell company (as defined in Exchange Act Rule 12b-2 of the Exchange Act).

☐ Yes ☒ No

The number of shares outstanding of the Issuer’s common stock as of August 11, 2021 was 6,371,190.

TABLE OF CONTENTS

	Page
PART I - FINANCIAL INFORMATION	3
Item 1. Unaudited Financial Statements	3
Consolidated Balance Sheets as of June 30, 2021 and December 31, 2020	3
Consolidated Statements of Operations for the Three and Six Months Ended June 30, 2021 and 2020	4
Consolidated Statements of Cash Flows for the Six Months Ended June 30, 2021 and 2020	5
Consolidated Statements of Changes in Stockholders’ Deficit for the Three and Six Months Ended June 30, 2021 and 2020	6
Notes to Unaudited Consolidated Financial Statements	8
Item 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations	27
Item 3. Quantitative and Qualitative Disclosure About Market Risk	32
Item 4. Controls and Procedures	32
PART II - OTHER INFORMATION	33
Item 1. Legal Proceedings	33
Item 1A. Risk Factors	33
Item 2. Unregistered Sales of Equity Securities and Use of Proceeds	34
Item 3. Defaults Upon Senior Securities	34
Item 4. Mine Safety Disclosures	34
Item 5. Other Information	34
Item 6. Exhibits	35
SIGNATURES	36

2

PART I - FINANCIAL INFORMATION

Item 1. Financial Statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY

CONSOLIDATED BALANCE SHEETS

(UNAUDITED)

	June 30, 2021			December 31, 2020
ASSETS
CURRENT ASSETS
Cash and cash equivalents	$	46,625		$	18,540
Accounts receivable		629,766			131,228
Inventories, net of $342,496 reserve at June 30, 2021 and December 31, 2020		492,095			592,767
Prepaid expenses and other current assets		219,161			314,936
Total current assets		1,387,647			1,057,471
Investment in equity securities		274,621			517,001
Property and equipment, net		7,431			16,490
Right of use asset leases		189,808			221,432
Intangible assets, net		447,115			490,385
TOTAL ASSETS	$	2,306,622		$	2,302,779
LIABILITIES AND STOCKHOLDERS’ DEFICIT
CURRENT LIABILITIES
Accounts payable	$	628,835		$	771,945
Accrued employee compensation		420,510			417,578
Accrued professional fees and other		2,163,563			2,037,806
Other current liabilities		8,165,281			6,330,722
Deferred revenue		49,189			47,328
Convertible debt, net of unamortized discounts of $2,353,152 and $3,948,167, respectively		10,909,314			7,545,670
Other debt, net of unamortized discounts of $5,495 and $0, respectively		1,363,931			1,135,469
Operating lease liability		69,204			65,193
Other related party debt		184,600			166,000
Total current liabilities		23,954,427			18,517,711
LONG TERM LIABILITIES
Long term debt		527,038			527,039
Operating lease liability – long term		120,604			156,239
Deferred revenue		7,446			19,382
TOTAL LIABILITIES		24,609,515			19,220,371
COMMITMENTS AND CONTINGENCIES (Note 4)
STOCKHOLDERS’ DEFICIT
Series D Convertible Preferred Stock, $.01 par value; 850 shares authorized; 300 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively (Liquidation value of $300,000)		3			3
Series G Convertible Preferred Stock, $.01 par value; 240,000 shares authorized; 80,570 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		806			806
Series H Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 10,000 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		100			100
Series H2 Convertible Preferred Stock, $.01 par value; 21 shares authorized; 21 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		-			-
Series J Convertible Preferred Stock, $.01 par value; 6,250 shares authorized; 3,458 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		35			35
Series K Convertible Preferred Stock, $.01 par value; 15,000 shares authorized; 6,880 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		68			68
Series AA Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 8,083 and 8,043 shares issued and outstanding on June 30, 2021 and December 31, 2020, respectively		81			81
Common stock, $.01 par value; 100,000,000 shares authorized; 6,260,884 and 4,168,324 shares issued and outstanding on June 30, 2021 and December 31, 2020 respectively		62,608			41,683
Warrants to acquire common stock		30,911,495			29,192,471
Additional paid-in capital		55,317,862			50,312,968
Accumulated deficit		(108,595,951	)		(96,465,807	)
Total stockholders’ deficit		(22,302,893	)		(16,917,592	)
TOTAL LIABILITIES AND STOCKHOLDERS’ DEFICIT	$	2,306,622		$	2,302,779

The accompanying notes are an integral part of these unaudited consolidated financial statements

3

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY

CONSOLIDATED STATEMENTS OF OPERATIONS

(UNAUDITED)

	For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2021			2020			2021			2020
Revenue:
Products, services, other	$	608,927		$	268,154		$	1,168,801		$	522,027
Total revenue		608,927			268,154			1,168,801			522,027
Costs and expenses:
Cost of products and services		286,660			134,882			512,935			310,028
Research and development		256,507			294,602			556,450			560,292
Selling and marketing		92,813			162,098			186,141			351,214
General and administrative		619,286			1,007,215			1,634,716			2,026,225
Total operating costs and expenses		1,255,266			1,598,797			2,890,242			3,247,759
Operating loss		(646,339	)		(1,330,643	)		(1,721,441	)		(2,725,732	)
Other (expense) income:
Interest expense, net		(3,526,141	)		(1,724,879	)		(8,194,205	)		(3,296,679	)
Unrealized (loss) gain on investment in equity securities		(134,477	)		196,891			(242,380	)		346,262
Loss on extinguishment of liabilities		(498,226	)		(1,710,151	)		(1,223,385	)		(2,846,518	)
Other income		60,012			-			58,653			-
Total other expense		(4,098,832	)		(3,238,139	)		(9,601,317	)		(5,796,935	)
Net loss		(4,745,171	)		(4,568,782	)		(11,322,758	)		(8,522,667	)
Deemed dividends on beneficial conversion feature		-			-			(57,884	)		-
Preferred stock dividends		(404,171	)		(396,970	)		(807,386	)		(721,556	)
Net loss attributable to common stockholders	$	(5,149,342	)	$	(4,965,752	)	$	(12,188,028	)	$	(9,244,223	)
Basic and diluted net loss per share attributable to common stockholders	$	(0.90	)	$	(1.70	)	$	(2.29	)	$	(3.34	)
Weighted average common stock shares outstanding used in the basic and diluted net loss per share calculation		5,748,711			2,914,659			5,312,172			2,765,132

The accompanying notes are an integral part of these unaudited consolidated financial statements

4

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY

CONSOLIDATED STATEMENTS OF CASH FLOWS

(UNAUDITED)

	For the Six Months Ended
	June 30,
	2021			2020
CASH FLOWS FROM OPERATING ACTIVITIES:
Net loss	$	(11,322,758	)	$	(8,522,667	)
Adjustments to reconcile net loss to net cash used in operating activities:
Gain on loan forgiveness		(367,039	)		-
Non-cash lease expense		31,624			35,927
Common stock and warrants issued for interest and extension fees		4,054,749			159,784
Depreciation and amortization		56,291			74,247
Accretion of interest and amortization of debt discount		3,909,024			1,750,911
Loss on extinguishment of accrued liabilities and debt		-			977,622
Stock-based compensation expense		124,695			307,110
Loss (Gain) on investment in equity securities		242,380			(346,262	)
Common stock issued for services		238,512			87,963
Changes in operating assets and liabilities:
Accounts receivable		(498,538	)		108,096
Inventories		100,672			2,853
Prepaid expenses and other assets		95,775			20,944
Accounts payable		(143,110	)		(5,927	)
Accrued employee compensation		2,932			(58,775	)
Operating lease liability		(31,624	)		(35,927	)
Deferred revenue and other accrued expenses		1,415,688			2,643,607
Net cash used in operating activities		(2,090,727	)		(2,800,494	)
CASH FLOWS FROM INVESTING ACTIVITIES:
Advance on loan receivable		-			(531,250	)
Purchases of property plant and equipment		(3,962	)		(1,663	)
Net cash used in investing activities		(3,962	)		(532,913	)
CASH FLOWS FROM FINANCING ACTIVITIES:
Net proceeds from Series AA Convertible Preferred Stock		100,000			-
Proceeds from stock option exercises		14,773			-
Net proceeds from convertible debt		2,598,250			4,422,600
Net proceeds from non-convertible debt – third party		1,183,188			990,539
Net proceeds from non-convertible debt – related party		171,600			8,500
Payments on convertible debt		(1,200,996	)		(1,257,250	)
Payments on non-convertible debt – related party		(153,000	)		-
Payments on non-convertible debt		(591,041	)		(819,149	)
Net cash provided by financing activities		2,122,774			3,345,240
NET INCREASE IN CASH AND CASH EQUIVALENTS		28,085			11,833
CASH AND CASH EQUIVALENTS AT BEGINNING OF YEAR		18,540			29,625
CASH AND CASH EQUIVALENTS AT END OF PERIOD	$	46,625		$	41,458
SUPPLEMENTAL INFORMATION
Interest paid in cash	$	383,403		$	419,817
NON CASH TRANSACTIONS:
Interest added to principal		-			152,552
Common stock issued to settle accrued liabilities		-			127,855
Common stock issued with debt		112,877			-
Discount from warrants issued with debt		1,068,842			2,958,693
Common stock issued in lieu of cash for dividend		114,298			176,748
Preferred stock dividends		807,386			721,556
Conversion of debt and interest into common stock		349,250			1,317,649
Discount due to beneficial conversion feature		566,847			982,097
Deemed dividend - beneficial conversion feature		57,884			-

The accompanying notes are an integral part of these unaudited consolidated financial statements

5

PRESSURE BIOSCIENCES, INC.

CONSOLIDATED STATEMENTS OF CHANGES IN STOCKHOLDERS’ DEFICIT

(UNAUDITED)

	Series D Preferred Stock				Series G Preferred Stock				Series H Preferred Stock				Series H(2) Preferred Stock				Series J Preferred Stock				Series K Preferred Stock				Series AA Preferred Stock				Common Stock				Stock		Additional Paid-In		Accumulated			Total Stockholders'
	Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Warrants		Capital		Deficit			Deficit
BALANCE, December 31, 2019		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		7,939	$	80		2,549,620	$	25,496	$	22,599,177	$	44,261,105	$	(78,942,277	)	$	(12,055,407	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		241,769		-			241,769
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(324,586	)		(324,586	)
Issuance of common stock to settle accrued liabilities		-		-		-		-		-		-		-		-		-		-		-		-		-		-		66,500		665		-		127,190		-			127,855
Common stock issued for debt settlement		-		-		-		-		-		-		-		-		-		-		-		-		-		-		10,000		100		-		24,900		-			25,000
Issuance of common stock for debt extension and interest paid in kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		38,521		385		-		60,175		-			60,560
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		404,608		-			404,608
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		1,205,010		-		-			1,205,010
Warrants issued for debt extension		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		609,143		-		-			609,143
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(3,953,885	)		(3,953,885	)
BALANCE, March 31, 2020		300	$	3		80,570	$	806		10,000	$	100		21		-		3,458	$	35		6,880	$	68		7,939	$	80		2,664,641	$	26,646		24,413,330		45,119,747		(83,220,748	)		(13,659,933	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		65,341		-			65,341
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(396,970	)		(396,970	)
Issuance of common stock for services		-		-		-		-		-		-		-		-		-		-		-		-		-		-		25,000		250		-		87,713		-			87,963
Issuance of common stock for interest paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		42,510		425		-		98,799		-			99,224
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		577,489		-			577,489
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		1,753,683		-		-			1,753,683
Warrants issued for debt extension		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		360,602		-		-			360,602
Issuance of Common Stock for Dividends Paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		64,388		644		-		176,104		-			176,748
Conversion of debt and interest for Common Stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		410,746		4,107		-		1,288,542		-			1,292,649
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(4,568,782	)		(4,568,782	)
BALANCE, June 30, 2020		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		7,939	$	80		3,207,285	$	32,072	$	26,527,615	$	47,413,735	$	(88,186,500	)	$	(14,211,986	)

6

	Series D Preferred Stock				Series G Preferred Stock				Series H Preferred Stock				Series H(2) Preferred Stock				Series J Preferred Stock				Series K Preferred Stock				Series AA Preferred Stock				Common Stock				Stock		Additional Paid-In			Accumulated			Total Stockholders’
	Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Warrants		Capital			Deficit			Deficit
BALANCE, December 31, 2020		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,043		81		4,168,324	$	41,683	$	29,192,471	$	50,312,968		$	(96,465,807	)	$	(16,917,592	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		61,237			-			61,237
Stock option exercise		-		-		-		-		-		-		-		-		-		-		-		-		-		-		21,411		214		-		14,559			-			14,773
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(403,215	)		(403,215	)
Issuance of warrants for interest paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		600,298		-			-			600,298
Issuance of common stock for services		-		-		-		-		-		-		-		-		-		-		-		-		-		-		112,400		1,124		-		237,388			-			238,512
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		53,777			-			53,777
Series AA Preferred Stock offering		-		-		-		-		-		-		-		-		-		-		-		-		40		-		-		-		49,884		50,116			-			100,000
Beneficial conversion option on convertible preferred stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		57,884			-			57,884
Deemed dividend on convertible preferred stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(57,884	)		-			(57,884	)
Conversion of debt and interest for common stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		47,200		472		-		117,528			-			118,000
Issuance of common stock for interest paid in kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		922,372		9,224		-		2,012,556			-			2,021,780
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		162,654		-			-			162,654
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(6,577,587	)		(6,577,587	)
BALANCE, March 31, 2021		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,083	$	81		5,271,707	$	52,717	$	30,005,307	$	52,860,129		$	(103,446,609	)	$	(20,527,363	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		63,458			-			63,458
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(404,171	)		(404,171	)
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		513,070			-			513,070
Issuance of common stock for interest paid in kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		720,610		7,206		-		1,425,465			-			1,432,671
Issuance of common stock for dividends paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		56,067		560		-		113,738			-			114,298
Conversion of debt and interest for common stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		92,500		925		-		230,325			-			231,250
Stock issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		120,000		1,200		-		111,677			-			112,877
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		906,188		-			-			906,188
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(4,745,171	)		(4,745,171	)
BALANCE, June 30, 2021		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,083	$	81		6,260,884	$	62,608	$	30,911,495	$	55,317,862		$	(108,595,951	)	$	(22,302,893	)

The accompanying notes are an integral part of these unaudited consolidated financial statements

7

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY

NOTES TO CONSOLIDATED FINANCIAL STATEMENTS

JUNE 30, 2021

(UNAUDITED)

1) Business Overview, Liquidity and Management Plans

Pressure BioSciences, Inc. (“we”, “our”, “the Company”) develops and sells innovative, broadly enabling, pressure-based platform solutions for the worldwide life sciences industry. Our solutions are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions (e.g., cell lysis, biomolecule extraction) safely and reproducibly. Our primary focus has historically been in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technology expertise in two new platform technology areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. for gently controlled disaggregation and refolding of biotherapeutic proteins (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies.

2) Going Concern

The accompanying financial statements have been prepared assuming that the Company will continue as a going concern, which contemplates the realization of assets and the liquidation of liabilities in the normal course of business. However, we have experienced losses from operations and negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of June 30, 2021, we do not have adequate working capital resources to satisfy our current liabilities and as a result, there is substantial doubt regarding our ability to continue as a going concern. We have been successful in raising debt and equity capital in the past and as described in Notes 5 and 6. In addition we raised debt and equity capital after June 30, 2021 as described in Note 7. We have financing efforts in place to continue to raise cash through debt and equity offerings. Although we have successfully completed financings and reduced expenses in the past, we cannot assure you that our plans to address these matters in the future will be successful. These financial statements do not include any adjustments that might result from this uncertainty.

8

3) Summary of Significant Accounting Policies

Basis of Presentation

The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2020.

Use of Estimates

The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements.

Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiary PBI BioSeq, Inc. All intercompany accounts and transactions have been eliminated in consolidation.

9

Revenue Recognition

We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, and ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers. Revenue is measured based on a consideration specified in a contract with a customer, and excludes any sales incentives and amounts collected on behalf of third parties. We enter into sales contracts that may consist of multiple distinct performance obligations where certain performance obligations of the sales contract are not delivered in one reporting period. We measure and allocate revenue according to ASC 606-10.

We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation.

Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue.

Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods.

Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue.

The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components.

Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements.

We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

a)	The fair value of the asset or service involved is not determinable.
b)	The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange.
c)	The transaction lacks commercial substance.

We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold.

We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases.

Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract.

10

Disaggregation of revenue

In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition.

In thousands of US dollars ($)	Three Months Ended June 30,				Six Months Ended June 30,
Primary geographical markets	2021		2020		2021		2020
North America	$	477	$	162	$	685	$	307
Europe		103		3		187		4
Asia		29		103		297		211
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Major products/services lines		2021		2020		2021		2020
Hardware	$	336	$	122	$	713	$	217
Consumables		44		51		146		107
Contract research services		136		34		142		44
Sample preparation accessories		40		33		69		58
Technical support/extended service contracts		34		17		58		36
Shipping and handling		16		6		35		15
Other		3		5		6		45
	$	609	$	268	$	1,169	$	522

11

	Three Months Ended June 30,				Six Months Ended June 30,
Timing of revenue recognition		2021		2020		2021		2020
Products transferred at a point in time	$	440	$	217	$	969	$	442
Services transferred over time		169		51		200		80
	$	609	$	268	$	1,169	$	522

Contract balances

In thousands of US dollars ($)	June 30, 2021		December 31, 2020
Receivables, which are included in ‘Accounts Receivable’	$	630	$	131
Contract liabilities (deferred revenue)		57		67

Transaction price allocated to the remaining performance obligations.

The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period.

 Schedule of Future Related to Performance Obligations

In thousands of US dollars ($)	2021		2022		Total
Extended warranty service	$	49		8	$	57

All consideration from contracts with customers is included in the amounts presented above.

Contract Costs

The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing.

12

Concentrations

Credit Risk

Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories.

The following table illustrates the level of concentration as a percentage of total revenues during the three and six months ended June 30, 2021 and 2020.

	For the Three Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		71	%
Federal Agencies		14	%		2	%

	For the Six Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		70	%
Federal Agencies		8	%		4	%

The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of June 30, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable.

	June 30, 2021			December 31, 2020
Top Five Customers		56	%		89	%
Federal Agencies		11	%		10	%

Product Supply

We utilize a contract assembler for our Barocycler® 2320EXT. They provide us with precision manufacturing services that include management support services to meet our specific application and operational requirements. Among the services provided to us are:

● CNC Machining

● Contract Assembly & Kitting

● Component and Subassembly Design

● Inventory Management

● ISO certification

At this time, we believe that outsourcing contract assembly of our Barocycler® 2320EXT is the most cost-effective method for us to obtain ISO Certified, CE and CSA Marked instruments. The Company also continues to evaluate the potential economic benefits of bringing instrument assembly in-house.

We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments.

13

Investment in Equity Securities

As of June 30, 2021, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company).

We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income.

As of June 30, 2021, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $274,621. We recorded $242,380 as an unrealized loss during the six months ended June 30, 2021 for changes in market value.

Computation of Loss per Share

Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss.

The following table illustrates our computation of loss per share for the three and six months ended June 30, 2021 and 2020:

	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2021			2020			2021			2020
Numerator:
Net loss attributable to common stockholders	$	(5,149,342	)	$	(4,965,752	)	$	(12,188,028	)	$	(9,244,223	)
Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding		5,748,711			2,914,659			5,312,172			2,765,132
Loss per common share – basic and diluted	$	(0.90	)	$	(1.70	)	$	(2.29	)	$	(3.34	)

14

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms.

	As of June 30,
	2021		2020
Stock options		1,350,046		1,392,370
Convertible debt		5,083,187		3,905,867
Common stock warrants		15,703,807		12,761,126
Convertible preferred stock:
Series D Convertible Preferred Stock		25,000		25,000
Series G Convertible Preferred Stock		26,857		26,857
Series H Convertible Preferred Stock		33,334		33,334
Series H2 Convertible Preferred Stock		70,000		70,000
Series J Convertible Preferred Stock		115,267		115,267
Series K Convertible Preferred Stock		229,334		229,334
Series AA Convertible Preferred Stock		8,083,000		7,939,000
		30,719,832		26,498,155

Accounting for Stock-Based Compensation Expense

We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant.

Determining Fair Value of Stock Option Grants

Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period.

Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted.

Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award.

Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term.

Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense.

15

The Company recognized stock-based compensation expense of $63,458 and $65,341 for the three months ended June 30, 2021 and 2020, respectively. The Company recognized stock-based compensation expense of $124,695 and $307,110 for the six months ended June 30, 2021 and 2020, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations:

	For the Three Months Ended				For the Six Months Ended
	June 30,				June 30,
	2021		2020		2021		2020
Cost of sales	$	5,107	$	5,107	$	10,160	$	13,063
Research and development		26,491		26,137		52,353		64,963
Selling and marketing		5,887		6,358		10,482		20,294
General and administrative		25,973		27,739		51,700		208,790
Total stock-based compensation expense	$	63,458	$	65,341	$	124,695	$	307,110

Fair Value of Financial Instruments

Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. Long-term liabilities include debt and deferred revenue with a carrying value that approximates fair value.

Fair Value Measurements

The Company follows the guidance of FASB ASC Topic 820, “Fair Value Measurements and Disclosures” (“ASC 820”) as it related to all financial assets and financial liabilities that are recognized or disclosed at fair value in the financial statements on a recurring basis.

The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement.

Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management.

16

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of June 30, 2021:

			Fair value measurements at June 30, 2021 using:
	June 30, 2021		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities	$	274,621	$	274,621		-		-
Total Financial Assets	$	274,621	$	274,621	$	-	$	-

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020:

			Fair value measurements at December 31, 2020 using:
	December 31, 2020		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities		517,001		517,001		-		-
Total Financial Assets	$	517,001	$	517,001	$	-	$	-

17

4) Commitments and Contingencies

Operating Leases

The Company accounts for its leases under ASC 842. The Company has elected to apply the short-term lease exception to leases of one year or less.

Our corporate office is currently located at 14 Norfolk Avenue, South Easton, Massachusetts 02375. We are currently paying $6,950 per month, on a lease extension, signed on December 30, 2020, that expires December 31, 2021, for our corporate office. We expanded our space to include offices, warehouse, and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current payments.

We extended our lease for our space in Medford, MA (the “Medford Lease”) from December 30, 2020 to December 30, 2023. The lease requires monthly payments of $7,282 subject to annual cost of living increases. The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.

The Company accounted for the lease extension of our Medford Lease as a lease modification under ASC 842. At the effective date of modification, the Company recorded an adjustment to the right-of-use asset and lease liability in the amount of $221,432 based on the net present value of lease payments discounted using an estimated borrowing rate of 12%.

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms as of June 30, 2021:

2021	$	85,391
2022		87,383
2023		87,383
Total minimum payments required	$	260,157

Target Discovery, Inc.

In the six months ended June 30, 2021, the Company incurred $34,400 in fees with Target Discovery, Inc. for the use of their facilities and other services.

5) Convertible Debt and Other Debt

Convertible Debt

On various dates during the six months ended June 30, 2021, the Company issued convertible notes for a total of $3,104,625 which contained varied terms and conditions as follows: a) 6-12 month maturity date; b) interest rates of 10-18%; c) convertible to the Company’s common stock at issuance at a fixed rate of $2.50 or at variable conversion rates upon an event of default. These notes were issued with either shares of common stock or warrants to purchase common stock that were fair valued at issuance date. The aggregate relative fair value of the shares of common stock and warrants issued with the notes of $1,181,719 was recorded as a debt discount to be amortized over the term of the notes. We then computed the effective conversion price of the notes, and recorded a $566,847 beneficial conversion feature as a debt discount to be amortized over the term of the notes. We also evaluated the convertible notes for derivative liability treatment and determined that the notes did not qualify for derivative accounting treatment at June 30, 2021.

18

The specific terms of the convertible notes and outstanding balances as of June 30, 2021 are listed in the tables below.

Inception Date		Term		Loan Amount		Outstanding balance with OID		Original Issue Discount (OID)		Interest Rate			Conversion Price		Deferred Finance Fees		Discount for conversion feature and warrants/shares
May 17, 2018 (1) (2)			12 months	$	380,000	$	166,703	$	15,200		8	%	$	2.50	$	15,200	$	332,407
June 8, 2018 (1) (4)	(1)		6 months	$	50,000	$	50,000	$	2,500		2	%	$	7.50	$	2,500	$	3,271
October 19, 2018 (1)			6 months	$	100,000	$	100,000	$	-		5	%	$	7.50	$	-	$	-
November 13, 2018 (1) (3) (4)			6 months	$	200,000	$	78,508	$	-		5	%	$	2.50	$	-	$	168,634
January 3, 2019 (1) (4)			6 months	$	50,000	$	50,000	$	2,500		24	%	$	7.50	$	2,500	$	-
February 21, 2019 (1) (2)			12 months	$	215,000	$	215,000	$	-		4	%	$	2.50	$	15,000	$	107,709
March 18, 2019 (1)			6 months	$	100,000	$	100,000	$	-		4	%	$	7.50	$	-	$	10,762
June 4, 2019 (1) (2)			9 months	$	500,000	$	302,484	$	-		8	%	$	2.50	$	40,500	$	70,631
June 19, 2019 (1) (2)			12 months	$	105,000	$	105,000	$	-		4	%	$	2.50	$	5,000	$	2,646
June 7, 2019 (1) (4)			6 months	$	125,000	$	6,746	$	-		5	%	$	7.50	$	-	$	18,254
July 1, 2019 (1) (2)			12 months	$	107,500	$	107,500	$	-		4	%	$	2.50	$	7,500	$	85,791
July 19, 2019 (1) (2)			12 months	$	115,000	$	115,000	$	-		4	%	$	2.50	$	5,750	$	15,460
July 19, 2019 (1) (2)			12 months	$	130,000	$	130,000	$	-		6	%	$	2.50	$	6,500	$	-
September 27, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	13,759
October 24, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	-
November 1, 2019 (1) (2)			12 months	$	270,000	$	270,000	$	-		6	%	$	2.50	$	13,500	$	-
November 15, 2019 (1)			12 months	$	385,000	$	320,000	$	35,000		10	%	$	2.50	$	35,000	$	90,917
January 2, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	91,606
January 24, 2020 (1)			12 months	$	247,500	$	247,500	$	22,500		10	%	$	2.50	$	22,500	$	89,707
January 29, 2020 (1)			12 months	$	363,000	$	363,000	$	33,000		10	%	$	2.50	$	33,000	$	297,000
February 12, 2020 (1)			12 months	$	275,000	$	275,000	$	25,000		10	%	$	2.50	$	25,000	$	225,000
February 19, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	135,000
March 11, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	232,810
March 13, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	60,705
March 26, 2020 (1)			12 months	$	111,100	$	111,100	$	10,100		10	%	$	2.50	$	10,100	$	90,900
April 8, 2020 (1)			12 months	$	276,100	$	276,100	$	25,100		10	%	$	2.50	$	25,000	$	221,654
April 17, 2020 (1)			12 months	$	143,750	$	143,750	$	18,750		10	%	$	2.50	$	-	$	96,208
April 30, 2020 (1)			12 months	$	546,250	$	546,250	$	71,250		10	%	$	2.50	$	47,500	$	427,500
May 6, 2020 (1)			12 months	$	460,000	$	460,000	$	60,000		10	%	$	2.50	$	40,000	$	360,000
May 18, 2020 (1)			12 months	$	546,250	$	221,250	$	46,250		10	%	$	2.50	$	35,500	$	439,500
June 2, 2020 (1)			12 months	$	902,750	$	652,750	$	92,750		10	%	$	2.50	$	58,900	$	708,500
June 12, 2020 (1)			12 months	$	57,500	$	57,500	$	7,500		10	%	$	2.50	$	5,000	$	45,000
June 22, 2020 (1)			12 months	$	138,000	$	138,000	$	18,000		10	%	$	2.50	$	12,000	$	108,000
July 7, 2020 (1)			12 months	$	586,500	$	586,500	$	76,500		10	%	$	2.50	$	51,000	$	400,234
July 17, 2020 (1)			12 months	$	362,250	$	362,250	$	47,250		10	%	$	2.50	$	31,500	$	185,698
July 29, 2020 (1)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	241,245
July 21, 2020 (1) (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	24,875
August 14, 2020 (1)			12 months	$	762,450	$	462,450	$	69,450		10	%	$	2.50	$	66,300	$	580,124
September 10, 2020			12 months	$	391,000	$	391,000	$	51,000		10	%	$	2.50	$	34,000	$	231,043
September 21, 2020 (5)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	66,375
September 23, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	20,500
September 25, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	-	$	19,125
December 3, 2020			12 months	$	299,000	$	299,000	$	39,000		10	%	$	2.50	$	26,000	$	197,882
October 22, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	18,875
December 23, 2020 (1) (5)			6 months	$	1,000,000	$	150,000	$	100,000		10	%	$	2.50	$	-	$	833,536
January 5, 2021 (1)			6 months	$	575,000	$	575,000	$	75,000		18	%	$	2.50	$	-	$	-
February 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	180,000
March 23, 2021			12 months	$	55,000	$	55,000	$	5,000		10	%	$	2.50	$	-	$	36,431
May 24, 2021			6 months	$	54,625	$	54,625	$	7,125		12	%	$	2.50	$	-	$	-
May 6, 2021			12 months	$	402,500	$	402,500	$	52,500		10	%	$	2.50	$	35,000	$	312,551
June 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	144,760
June 25, 2021			12 months	$	977,500	$	977,500	$	127,500		10	%	$	2.50	$	-	$	773,802
May 20, 2021			12 months	$	180,000	$	180,000	$	30,000		10	%	$	2.50	$	15,000	$	25,824
June 3, 2021			12 months	$	50,000	$	50,000	$	1,500		12	%	$	2.50	$	-	$	7,948
June 28, 2021			12 months	$	350,000	$	350,000	$	35,000		12	%		(6)	$	22,750	$	267,250
						$	13,262,466	$	1,502,225						$	972,500	$	9,117,409

(1)	The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.
(2)	The Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below).
(3)	Interest was capitalized and added to outstanding principal.
(4)	During the year ended December 31, 2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.
(5)	The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.
(6)	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.

As of June 30, 2021 one lender holds approximately $9.1 million of the $13.3 million convertible notes outstanding.

For the six months ended June 30, 2021, the Company recognized amortization expense related to the debt discounts indicated above of $3,864,956. The unamortized debt discounts as of June 30, 2021 related to the convertible debentures and other convertible notes amounted to $2,353,152.

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Standstill and Forbearance Agreements

The Company has entered into Standstill and Forbearance Agreements with lenders who hold convertible notes with a total principal as of June 30, 2021 of $1.57 million. Pursuant to the Standstill and Forbearance Agreements, the lenders agreed to not convert any portion of their notes into shares of common stock at a variable rate until March 31, 2021 for convertible notes with a principal balance of $469 thousand and until April 16, 2021 for convertible notes with a principal balance of $1.1 million. For the six months ended June 30, 2021, the Company incurred fees of approximately $1.0 million in connection with these agreements. After June 30, 2021 the Company settled $827,500 of these loans (see Note 7).

Convertible Loan Modifications and Extinguishments

We refinanced certain convertible loans during the three months ended June 30, 2021 at substantially the same terms for extensions ranging over a period of three to six months. We amortized any remaining unamortized debt discount as of the modification date over the remaining, extended term of the new loans. We applied ASC 470 of modification accounting to the debt instruments which were modified during the quarter or those settled with new notes issued concurrently for the same amounts but different maturity dates. The terms such as the interest rate, prepayment penalties, and default rates will be the same over the new extensions. According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications.

The cash flows of new debt exceeded 10% of the remaining cash flows of the original debt on several loans. During the six months ended June 30, 2021 we recorded losses on extinguishment of liabilities of approximately $1.6 million by calculating the difference of the fair value of the new debt and the carrying value of the old debt.

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The following table provides a summary of the changes in convertible debt, net of unamortized discounts, during 2021:

	2021
Balance at January 1,	$	7,545,670
Issuance of convertible debt, face value		3,104,625
Deferred financing cost		(506,375	)
Beneficial conversion feature on convertible note		(566,847	)
Debt discount from shares and warrants issued with debt		(1,181,719	)
Payments		(1,200,996	)
Conversion of debt into equity		(150,000	)
Accretion of interest and amortization of debt discount to interest expense		3,864,956
Balance at June 30,		10,909,314
Less: current portion		10,909,314
Convertible debt, long-term portion	$	–

Other Notes

On September 9, 2019 and February 28, 2020 we received a total of $966,500 unsecured non-convertible loans from a private investor with a one-month term. During the year ended December 31, 2020, the Company received net proceeds of $463,500, issued 150,000 warrants to purchase common stock (five-year term and $3.50 exercise price) and repaid $275,000. The relative fair value of $185,660 of the warrants issued with the note was recorded as a debt discount to be amortized over the term of the notes. As of June 30, 2021, the Company owes $691,500 on these notes which are past due. The Company and the investor are negotiating in good faith to extend the loans.

On October 1, 2019, the Company and the holder of the $170,000 non-convertible loan issued in May 2017 agreed to extend the term of the loan to December 31, 2019. The Company agreed to issue 1,200 shares of its common stock per month while the note remains outstanding. The note will continue to earn 10% annual interest. The loan is currently past due, and the Company and the investor are negotiating in good faith to extend the loan.

On October 11, 2019 we received a non-convertible loan with a one-month term and a 2% interest charge for $25,000 from a private investor. The loan is past due, and the Company and the investor are negotiating in good faith to extend the loan.

Merchant Agreements

We have signed various Merchant Agreements which are secured by second position rights to all customer receipts until the loan has been repaid in full and subject to interest rates of 9.3% - 11.5% per month. As illustrated in the following table, under the terms of these agreements, we received the disclosed Purchase Price and agreed to repay the disclosed Purchase Amount, which is collected by the Merchant lenders at the disclosed Daily Payment Rate. The Company’s Chief Executive Officer is personally guaranteeing the performance of the merchant loans.

The following table shows our Merchant Agreements as of June 30, 2021:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
February 4, 2021		125,000		165,000		55,281		1,032		-
March 11, 2021		125,000		167,500		40,559		1,396		2,500
March 26, 2021		240,000		330,960		129,952		2,364		-
May 3, 2021		200,000		275,800		141,458		1,970		5,000
June 2, 2021		135,000		186,165		115,677		1,400		1,350
	$	825,000	$	1,125,425	$	482,927	$	8,162	$	8,850

The following table shows our Merchant Agreements as of December 31, 2020:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
November 5, 2020	$	200,000	$	275,800	$	163,955		1,724	$	-
November 19, 2020		100,000		137,900		85,013		985		-
	$	300,000	$	413,700	$	248,968	$	2,709	$	-

We have accounted for the Merchant Agreements as loans under ASC 860 because while we provided rights to current and future receipts, we still had control over the receipts. The difference between the Purchase Amount and the Purchase Price is imputed interest that is recorded as interest expense when paid each day.

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Related Party Notes

In June 2018, we received a non-convertible loan of $15,000 from a private investor. The loan includes a one-year term and 15% guaranteed interest. This loan remains outstanding at June 30, 2021 and is currently past due.

During the six months ended June 30, 2021, we received short-term non-convertible loans of $171,600 from related parties and repaid $153,000 of related party loans. These notes bear interest ranging from 10% to 15% and are due upon demand.

Long term debt

During the six months ended June 30, 2021, the Company borrowed $367,038 through a COVID-19 program that was sponsored by the United States and administered by the Small Business Administration (the “SBA”). The most notable programs were the Payroll Protection Program (or “PPP”) and the Economic Injury Disaster Loan program (or “EIDL”). PPP loan has a 1% interest rate and a five-year term. During this period, the Company’s first PPP loan borrowed in 2020 ($367,039) was forgiven by the SBA. This gain was reported in losses on extinguishment of liabilities on the consolidated statements of operations.

The Company’s EIDL loan, $150,000, accrues interest at 3.75% and requires monthly payments of $731 for principal and interest beginning in June 2021. The balance of the principal will be due in 30 years. In connection with the EIDL loan the Company entered into a security agreement with the SBA, whereby the Company granted the SBA a security interest in all of the Company’s right, title and interest in all of the Company’s assets.

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  6) Stockholders’ Deficit

Preferred Stock

We are authorized to issue 1,000,000 shares of preferred stock with a par value of $0.01. Of the 1,000,000 shares of preferred stock:

1)	20,000 shares have been designated as Series A Junior Participating Preferred Stock (“Junior A”)
2)	313,960 shares have been designated as Series A Convertible Preferred Stock (“Series A”)
3)	279,256 shares have been designated as Series B Convertible Preferred Stock (“Series B”)
4)	88,098 shares have been designated as Series C Convertible Preferred Stock (“Series C”)
5)	850 shares have been designated as Series D Convertible Preferred Stock (“Series D”)
6)	500 shares have been designated as Series E Convertible Preferred Stock (“Series E”)
7)	240,000 shares have been designated as Series G Convertible Preferred Stock (“Series G”)
8)	10,000 shares have been designated as Series H Convertible Preferred Stock (“Series H”)
9)	21 shares have been designated as Series H2 Convertible Preferred Stock (“Series H2”)
10)	6,250 shares have been designated as Series J Convertible Preferred Stock (“Series J”)
11)	15,000 shares have been designated as Series K Convertible Preferred Stock (“Series K”)
12)	10,000 shares have been designated as Series AA Convertible Preferred Stock (“Series AA”)

As of June 30, 2021, there were no shares of Junior A, and Series A, B, C and E issued and outstanding. See our Annual Report on Form 10-K for the year ended December 31, 2020 for the pertinent disclosures of preferred stock.

During the six months ended June 30, 2021, the Company entered into Securities Purchase Agreements with accredited investors pursuant to which the Company sold an aggregate of 40 shares of Series AA Convertible Preferred Stock, each preferred share convertible into 1,000 shares of the Company’s common stock, par value $0.01 per share, for an aggregate Purchase price of $100,000. We issued to the investors warrants to purchase an aggregate 40,000 shares of common stock with an exercise price of $3.50 per share. The Company did not incur any placement agent fees for this transaction.

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Stock Options and Warrants

At the Company’s December 12, 2013 Special Meeting, the shareholders approved the 2013 Equity Incentive Plan (the “2013 Plan”) pursuant to which 3,000,000 shares of our common stock were reserved for issuance upon exercise of stock options or other equity awards. Under the 2013 Plan, we may award stock options, shares of common stock, and other equity interests in the Company to employees, officers, directors, consultants, and advisors, and to any other persons the Board of Directors deems appropriate. As of June 30, 2021, options to acquire 1,350,046 shares were outstanding under the Plan.

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As of June 30, 2021, total unrecognized compensation cost related to the unvested stock-based awards was $218,339, which is expected to be recognized over weighted average period of 1.62 years. The aggregate intrinsic value associated with the options outstanding and exercisable, and the aggregate intrinsic value associated with the warrants outstanding and exercisable as of June 30, 2021, based on the June 30, 2021 closing stock price of $2.17, was $1,472,943.

The following table summarizes information concerning options and warrants outstanding and exercisable:

	Stock Options					Warrants
	Weighted					Weighted
	Average					Average
	Shares			price per share		Shares			price per share		Shares			Total Exercisable
Balance outstanding, December 31, 2020		1,355,901		$	0.69		14,434,702		$	3.50		15,790,603			15,302,830
Granted		24,000			2.17		1,374,600			3.61		1,398,600			-
Exercised		(21,411	)		0.69		-			-		(21,411	)		-
Expired/forfeited		(8,444	)		0.69		(105,495	)	$	3.50		(113,939	)		-
Balance outstanding, June 30, 2021		1,350,046		$	0.72		15,703,807		$	3.50		17,053,853			16,699,039

In the six months ended June 30, 2021 the Company issued 24,000 stock options to an employee ($49,135 fair value, $2.17 exercise price, three-year vesting term and ten-year expiration term). As of June 30, 2021, the 1,350,046 stock options outstanding have a $0.72 weighted average exercise price and 8.22 years weighted average remaining term. Of these options, 995,232 are currently exercisable.

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Common Stock and Warrant Issuances

As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates.

	Loan Issuance	Loan		Shares		Defined		Defined
Loan	Date	Principal		Issuable		Date		Frequency
Loan 1	July 21, 2020	$	115,000		5,000		September 30, 2020		Monthly
Loan 2	September 21, 2020	$	345,000		12,500		November 16, 2020		Weekly
Loan 3	September 23, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 4	September 25, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 5	October 22, 2020	$	115,000		12,500		December 1, 2020		Weekly

For our loan dated December 23, 2020, we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the six months ended June 30, 2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five-year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021.

During the six months ended June 30, 2021, we issued 1,642,982 shares of common stock with a fair value of approximately $3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 139,700 shares with a fair value of $349,250 for conversions of debt principal and interest, 21,411 shares for stock option exercises (at an exercise price of $0.69 per share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value of $112,877 for Common Stock issued with debt. During this period, we also issued 1,374,600 warrants (three to five-year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $1.7 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind.

During the six months ended June 30, 2020, we issued to Series AA holders 64,388 shares of common stock for dividends totaling of $176,748 issued in stock in lieu of cash. During this period the Company also issued 593,277 shares of restricted common stock at a fair value of $1,693,251 to accredited investors and consultants. 420,746 of the shares with a fair value of $1,317,649 were issued for conversions of debt principal and interest; 81,031 of the shares with a fair value of $159,784 were issued for debt extensions and interest payments; 66,500 shares with a fair value of $127,855 were issued to settle an accrued liability; and 25,000 shares with a fair value of $87,963 were issued for services rendered.

  7) Subsequent Events

From July 1, 2021 through August 10, 2021 the Company received seven new fixed rate convertible loans for a total of $1.5 million. The Company issued 125,000 shares of common stock and 161,000 warrants (3-year and 5-year lives and $3.50 strike price) as fees paid to lenders. These loans have fixed conversion rates of $2.50-$3.00 (one has a variable conversion rate), carry annual interest rates ranging from 10% to 12%, and terms ranging from six to twelve months. One of the loans (for $735,000) is secured by the assets of PBI Agrochem, Inc., a newly incorporated subsidiary of the Company.

In this time period, the Company retired loans with a principal amount of $973,408 (dated June 8, 2018, November 13, 2018, February 21, 2019, June 19, 2019, July 1, 2019, July 19, 2019, and November 1, 2019) for $167,954 and 763,420 shares of common stock (settling principal, interest and fees) and reduced the principal of another loan with a $100,000 cash payment. After this activity, the Company had $741,687 of principal outstanding with two lenders subject to the standstill and forbearance agreements, which expired March 31, 2021 (see Note 5).

The Company is obligated to issue 665,000 shares of common stock to certain lenders for the quarter ended June 30, 2021, but has not issued the shares. The Company and the lenders are negotiating in good faith to resolve these loans and expect to reach a settlement in the coming month.

During this period, the Company also received $900,000 from the sale of Series AA shares (issuing 360,000 warrants with a five-year term and strike price of $3.50), and repaid $103,600 of related party loans.

In this time period, the Company also entered into a Merchant Cash lender agreement (collecting $66,677) and repaid an existing Merchant Cash lender for $62,832. Under this agreement, the Company pays $1,278 each business day to the lender. During this period, the Company also borrowed and repaid a $300,000 line-of-credit from a second lender.

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ITEM 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations

CAUTIONARY NOTE REGARDING FORWARD-LOOKING STATEMENTS

This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended (the “Securities Act”) and Section 21E of the Securities Exchange Act of 1934, as amended (the “Exchange Act”). In some cases, forward-looking statements are identified by terms such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “anticipates,” “believes,” “estimates,” “projects,” “predicts,” “potential” and similar expressions intended to identify forward-looking statements. Such statements include, without limitation, statements regarding:

● our need for, and our ability to raise, additional equity or debt financing on acceptable terms, if at all;

● our need to take additional cost reduction measures, cease operations or sell our operating assets, if we are unable to obtain sufficient additional financing;

● our belief that we will have sufficient liquidity to finance normal operations for the foreseeable future;

● the options we may pursue in light of our financial condition;

● the potential applications for Ultra Shear Technology (UST);

● the potential applications of the BaroFold high-pressure protein refolding and disaggregation technology

● the amount of cash necessary to operate our business;

● the anticipated uses of grant revenue and the potential for increased grant revenue in future periods;

● our plans and expectations with respect to our continued operations;

● the expected increase in the number of pressure cycling technology (“PCT”) and constant pressure (“CP”) based units that we believe will be installed and the expected increase in revenues from the sale of consumable products, extended service contracts, and biopharma contract services;

● our belief that PCT has achieved initial market acceptance in the mass spectrometry and other markets;

● the expected development and success of new instrument and consumables product offerings;

● the potential applications for our instrument and consumables product offerings;

● the expected expenses of, and benefits and results from, our research and development efforts;

● the expected benefits and results from our collaboration programs, strategic alliances and joint ventures;

● our expectation of obtaining additional research grants from the government in the future;

● our expectations of the results of our development activities funded by government research grants;

● the potential size of the market for biological sample preparation, biopharma contract services and Ultra Shear Technology;

● general economic conditions;

● the anticipated future financial performance and business operations of our company;

● our reasons for focusing resources in the market for genomic, proteomic, lipidomic and small molecule sample preparation;

● the importance of mass spectrometry as a laboratory tool;

● the advantages of PCT over other current technologies as a method of biological sample preparation and protein characterization in biomarker discovery, forensics, and histology, as well as for other applications;

● the capabilities and benefits of our PCT Sample Preparation System, consumables and other products;

● our belief that laboratory scientists will achieve results comparable with those reported to date by certain research scientists who have published or presented publicly on PCT and our other products and services;

● our ability to retain our core group of scientific, administrative and sales personnel; and

● our ability to expand our customer base in sample preparation and for other applications of PCT, as well as for our other products and services in both the BaroFold and Ultra Shear Technology areas.

These forward-looking statements are only predictions and involve known and unknown risks, uncertainties and other factors that may cause our actual results, levels of activity, performance or achievements to be materially different from any future results, levels of activity, performance or achievements, expressed or implied, by such forward-looking statements. Also, these forward-looking statements represent our estimates and assumptions only as of the date of this Quarterly Report on Form 10-Q. Except as otherwise required by law, we expressly disclaim any obligation or undertaking to release publicly any updates or revisions to any forward-looking statement contained in this Quarterly Report on Form 10-Q to reflect any change in our expectations or any change in events, conditions or circumstances on which any of our forward-looking statements are based. Factors that could cause or contribute to differences in our future financial and other results include those discussed in the risk factors set forth in Part I, Item 1A of our Annual Report on Form 10-K for the year ended December 31, 2020 and in this Report. We qualify all of our forward-looking statements by these cautionary statements.

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OVERVIEW:

We are a leader in the development and sale of innovative, broadly enabling, pressure-based platform solutions for the worldwide life sciences industry. Our solutions are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus has historically been in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technology expertise in two new platform technology areas: (1) the use of our patented technology from BaroFold, Inc. for gently controlled disaggregation and refolding of biotherapeutic proteins (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies.

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Developments and Accomplishments:

We reported the following accomplishments during the first half of 2021:

● On May 13, 2021, PBI was awarded three additional patents for its revolutionary Ultra Shear Technology™ (UST™) platform, all entitled “System for High Pressure, High Shear Processing of Fluids”. The new patents, awarded in Japan (No. 6843063), Australia (No. 2016243553), and China (ZL201680026865.2), bring the Company’s Intellectual Property (“IP”) estate to a total of 6 issued patents for UST and 29 issued patents overall.

● On April 14, 2021, PBI finalized terms and executed a new letter of intent to purchase the assets of an internationally-based developer and supplier of organically natural, eco-friendly agrochemicals.

● On March 15, 2021, PBI discussed partnerships with Leica Microsystems (cancer diagnostics) and Ohio State University (food industry consortium) with the Stock Day Podcast.

● On March 4, 2021, PBI reported that the transformative impact of the food industry consortium formed by Pressure BioSciences and Ohio State University was discussed in a showcase video from Emerging Technology Insider.

● On February 24, 2021, PBI and Ohio State University announced the formation of a food industry consortium to advance commercialization of the Company’s Ultra Shear Technology (UST) platform.

● On February 8, 2021, PBI announced plans to acquire the assets of a global eco-friendly agrochemical supplier.

● On January 20, 2021, PBI targeted a revolution in effectiveness of therapeutics via improved drug delivery and dosing safety when the Company announced a collaboration with SinuSys Corp to improve and optimize their lead sinus health product candidate prior to Phase IIb trials.

29

Results of Operations

The following disclosure compares the results of operations for the quarter ended June 30, 2021 (“Q2 2021”) with June 30, 2020 (“Q2 2020”) and compares the six months ended June 30, 2021 with June 30, 2020.

Products and Services Revenue

We recognized total revenue of $608,927 for Q2 2021 compared to $268,154 for Q2 2020, a 127% increase. For the year-to-date periods ending on June 30, 2021 and 2020 we recognized total revenue of $1,168,801 and $522,027, respectively, a 124% increase.

Cost of Products and Services

The cost of products and services was $286,660 for Q2 2021 compared to $134,882 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 our cost of products and services was $512,935 and $310,028, respectively. Gross profit margin on products and services increased from a larger portion of biopharma services that have higher profit margins (to 52.9% in Q2 2021 from 49.6% in Q2 2020 and to 56.1%in the year-to-date period ended June 30, 2021 from 40.6% in the year-to-date period ended June 30, 2020).

Research and Development

Research and development expenses were $256,507 for Q2 2021 compared to $294,602 for Q2 2020. The reported decrease of 13% was attributable to a decrease in outside services. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $556,450 and $560,292, respectively. The reported decrease was 1%.

Selling and Marketing

Selling and marketing expenses were $92,813 for Q2 2021 compared to $162,098 for Q2 2020. The reported decreases were primarily attributable to reduced employees in sales and marketing. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $186,141 and $351,214, respectively.

General and Administrative

General and administrative expenses were $619,286 for Q2 2021 compared to $1,007,215 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $1,634,716 and $2,026,225 respectively. The reported decreases were attributable to lower investor relations expenses.

Operating Loss

Operating loss was $646,339 for Q2 2021 compared to $1,330,643 for Q2 2020, a reduction of $684,304, or 51.4%. For the year to date periods ending of June 30, 2021 and 2020 the operating loss was $1,721,441 and $2,725,732, respectively, a $1,004,291 reduction, or 36.8%. The reported decreases were primarily attributable to revenue increases and decreases in costs and expenses in 2021.

Interest Expense, net

Interest expense was $3,526,141 for Q2 2021 compared to $1,724,879 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $8,194,205 and $3,296,679, respectively. The increases were primarily attributable to the increase in convertible and other debt and the issuance of common stock for interest paid-in-kind.

Unrealized (loss) gain on investment in equity securities

Unrealized loss on investments in equity securities was $134,477 for Q2 2021 compared to an unrealized gain of $196,891 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 the unrealized loss was $242,380 and an unrealized gain of $346,262, respectively. The reported change was attributable to movement in the market price of the Company’s investment in Nexity.

Loss on extinguishment of liabilities

In connection with debt extensions and forgiveness, we recognized net losses of $498,226 for Q2 2021 compared to $1,710,151 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these losses were $1,223,385 and $2,846,518, respectively. The decreases were principally related to the gain recognized on the forgiveness of our first PPP loan of $367,039.

Net loss attributable to common stockholders

Net loss attributable to common stockholders was $5,149,342 ($0.90 per share) for Q2 2021 compared to $4,965,752 ($1.70 per share) for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 the losses were $12,188,028 ($2.29 per share) and $9,244,223 ($3.34 per share), respectively. The decreases in loss per share were principally attributable to the increase in weighted shares outstanding.

30

Liquidity and Financial Condition

We have experienced negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of June 30, 2021, we did not have adequate working capital resources to satisfy our current liabilities and as a result, we have substantial doubt regarding our ability to continue as a going concern. As described in Notes 5 and 6 of the accompanying consolidated financial statements, we have been successful in raising debt and equity capital. We received $4.0 million in net proceeds from loans and sales of preferred stock in the six months ended June 30, 2021. We have efforts in place to continue to raise cash through debt and equity offerings. (See Note 7 to the financial statements)

We will need substantial additional capital to fund our operations in future periods. If we are unable to obtain financing on acceptable terms, or at all, we will likely be required to cease our operations, pursue a plan to sell our operating assets, or otherwise modify our business strategy, which could materially harm our future business prospects.

Net cash used in operations for the six months ended June 30, 2021 was $2,090,727 as compared to $2,800,494 for the three months ended June 30, 2020.

Net cash used in investing activities for the six months ended June 30, 2021 was $3,962 compared to $532,913 in the six months ended June 30, 2020.

Net cash provided by financing activities for the six months ended June 30, 2021 was $2,122,774 as compared to $3,345,240 for the six months ended June 30, 2020. The cash flows from financing activities in the six months ended June 30, 2021 included $100,000 from the sale of Series AA preferred stock, $4.0 million loan proceeds from convertible debt and other debt. In this period, cash flow from financing was reduced by debt payments of $1,200,996 on convertible debt, and $744,041 on other debt.

31

ITEM 3. QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK

This Item 3 is not applicable to us as a smaller reporting company and has been omitted.

ITEM 4. CONTROLS AND PROCEDURES

We maintain disclosure controls and procedures that are designed to ensure that information required to be disclosed in our Securities Exchange Act of 1934 filings are recorded, processed, summarized and reported within the time periods specified in the SEC’s rules and forms, and that such information is accumulated and communicated to our management, including our President and Chief Executive Officer (Principal Executive Officer) and Chief Financial Officer (Principal Financial Officer), as appropriate, to allow timely decisions regarding required disclosure. In designing and evaluating the disclosure controls and procedures, management recognized that controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives, as ours are designed to do, and management was necessarily required to apply its judgment in evaluating the cost-benefit relationship of possible controls and procedures.

As of June 30, 2021, we carried out an evaluation, under the supervision and with the participation of our management, including our Principal Executive Officer and Principal Financial Officer, of the effectiveness of the design and operation of our disclosure controls and procedures, as defined in Rules 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934. Based upon that evaluation, our Principal Executive Officer and Principal Financial Officer concluded that our disclosure controls and procedures were not effective.

Our conclusion that our disclosure controls and procedures were not effective as of June 30, 2021 is due to the continued presence of the material weaknesses in our internal control over financial reporting identified in our Annual Report on Form 10-K for the year ended December 31, 2020. These material weaknesses are the following:

●	We identified a lack of sufficient segregation of duties. Specifically, this material weakness is such that the design over these areas relies primarily on detective controls and could be strengthened by adding preventative controls to properly safeguard Company assets.
●	Management has identified a lack of sufficient personnel in the accounting function due to our limited resources with appropriate skills, training and experience to perform the review processes to ensure the complete and proper application of generally accepted accounting principles, particularly as it relates to valuation of warrants and other complex debt /equity transactions. Specifically, this material weakness resulted in audit adjustments to the annual consolidated financial statements and revisions to related disclosures, valuation of warrants and other equity transactions.
●	Limited policies and procedures that cover recording and reporting of financial transactions.
●	Lack of multiple levels of review over the financial reporting process
We continue to plan to remediate those material weaknesses as follows:
●	Improve the effectiveness of the accounting group by augmenting our existing resources with additional consultants or employees to assist in the analysis and recording of complex accounting transactions, and to simultaneously achieve desired organizational structuring for improved segregation of duties. We plan to mitigate this identified deficiency by hiring an independent consultant once we generate significantly more revenue or raise significant additional working capital.
●	Improve expert review and achieve desired segregation procedures by strengthening cross approval of various functions including quarterly internal audit procedures where appropriate once we generate significantly more revenue or raise significantly more working capital.

During the period covered by this Report, we implemented and performed additional substantive procedures, such as supervisory review of work papers and consistent use of financial models used in equity valuations, to ensure our consolidated financial statements as of and for the three-month period ended June 30, 2021, are fairly stated in all material respects in accordance with GAAP. We have not, however, been able to fully remediate the material weaknesses due to our limited financial resources. Our remediation efforts are largely dependent upon our securing additional financing to cover the costs of implementing the changes required. If we are unsuccessful in securing such funds, remediation efforts may be adversely affected in a material manner.

Except as described above, there have been no changes in our internal controls over financial reporting that occurred during the period ended June 30, 2021 that have materially affected, or are reasonably likely to materially affect, our internal controls over financial reporting.

32

PART II. OTHER INFORMATION

Item 1. Legal Proceedings

We are not currently involved in any litigation that we believe could have a material adverse effect on our financial condition or results of operations. There is no action, suit, proceeding, inquiry or investigation before or by any court, public board, government agency, self-regulatory organization or body pending or, to the knowledge of the executive officers of our company or any of our subsidiaries, threatened against or affecting our company, our common stock, any of our subsidiaries or of our companies or our subsidiaries’ officers or directors in their capacities as such, in which an adverse decision could have a material adverse effect.

Item 1A. Risk Factors

Factors that could cause or contribute to differences in our future financial and operating results include those discussed in the risk factors set forth in Item 1 of our Annual Report on Form 10-K for the year ended December 31, 2020 and in this Item 1A. The risks described in our Form 10-K and this Report are not the only risks that we face. Additional risks not presently known to us or that we do not currently consider significant may also have an adverse effect on the Company. If any of the risks actually occur, our business, results of operations, cash flows or financial condition could suffer.

There have been no material changes to the risk factors set forth in Item 1A of our 10-K for the year ended December 31, 2020 other than the following:

We owe over $9 million to one lender with such loans secured by a security interest in all of our assets. If we default under our obligations pursuant to such loans, the lender could foreclose on all of our assets which could require us to cease our operations.

Through June 30, 2021, we have issued Notes to the same holder such that the current gross amount owed to the holder is approximately $9.1 million. Our obligations under the Notes and the transaction documents relating to the Notes are secured by a security interest in all of our assets. As a result, if we default under our obligations under the Notes or the transaction documents, the holders of the Notes, acting through their appointed agent, could foreclose on their security interests and liquidate some or all of these assets, which could harm our business, financial condition and results of operations and could require us to reduce or cease operations. In addition, the pledge of these assets and other restrictions may limit our flexibility in raising capital for other purposes. Because all of our assets are pledged under these financing arrangements, our ability to incur additional secured indebtedness or to sell or dispose of assets to raise capital may be impaired, which could have an adverse effect on our financial flexibility.

33

The holders of our Common Stock could suffer substantial dilution due to our corporate financing practices.

The holders of our common stock could suffer substantial dilution due to our corporate financing practices which, in the past few years has included private placements. As of June 30, 2021, we had 6,260,884 shares outstanding. As of June 30, 2021, if all of the outstanding shares of Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H Convertible Preferred Stock, Series H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock and Series AA Convertible Preferred Stock were converted into shares of common stock and all outstanding options and warrants to purchase shares of common stock were exercised and all fixed rate convertible notes and debentures were converted, each as of June 30, 2021, an additional 30,719,832 shares of common stock would be issued and outstanding. The full cash exercise of the options and warrants would result in approximately $55.9 million in cash proceeds to the Company. This additional issuance of shares of common stock would cause immediate and substantial dilution to our existing stockholders and could cause a significant reduction in the market price of our common stock.

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds

Except where noted, all the securities discussed in this Part II, Item 2 were issued in reliance on the exemption under Section 4(a)(2) of the Securities Act.

During the six months ended June 30, 2021, we issued 1,642,982 shares of common stock with a fair value of approximately $3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 139,700 shares with a fair value of $349,250 for conversions of debt principal and interest, 21,411 shares for stock option exercises (at an exercise price of $0.69 per share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value of $112,877 for Common Stock issued with debt. During this period, we also issued 1,374,600 warrants (three to five-year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $1.7 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind.

During the six months ended June 30, 2020, we issued to Series AA holders 64,388 shares of common stock for dividends totaling of $176,748 issued in stock in lieu of cash. During this period the Company also issued 593,277 shares of restricted common stock at a fair value of $1,693,251 to accredited investors and consultants. 420,746 of the shares with a fair value of $1,317,649 were issued for conversions of debt principal and interest; 81,031 of the shares with a fair value of $159,784 were issued for debt extensions and interest payments; 66,500 shares with a fair value of $127,855 were issued to settle an accrued liability; and 25,000 shares with a fair value of $87,963 were issued for services rendered.

Item 3. Defaults upon Senior Securities

None.

Item 4. Mine Safety Disclosures

Not applicable.

Item 5. Other Information

None.

34

Item 6. Exhibits

Exhibits
31.1*	Certification by the Principal Executive Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))
31.2*	Certification by the Principal Financial Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))
32.1**	Certification by the Principal Executive Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*
32.2**	Certification by the Principal Financial Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*
101.INS*	XBRL Instance Document
101.SCH*	XBRL Taxonomy Extension Schema Document
101.CAL*	XBRL Taxonomy Extension Calculation Linkbase Document
101.DEF*	XBRL Taxonomy Extension Definition Linkbase Document
101.LAB*	XBRL Taxonomy Extension Label Linkbase Document
101.PRE*	XBRL Taxonomy Extension Presentation Linkbase Document

* Filed herewith.

** In accordance with SEC Release 33-8238, Exhibits 32.1 and 32.2 are furnished and not filed.

35

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant caused this report to be signed on its behalf by the undersigned thereunto duly authorized.

	PRESSURE BIOSCIENCES, INC.
Date: August 18, 2021	By:	/s/ Richard T. Schumacher
		Richard T. Schumacher
		President & Chief Executive Officer
		(Principal Executive Officer and Principal Financial Officer)

36

EX-31.1

EXHIBIT 31.1

CERTIFICATION PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

I, Richard T. Schumacher, certify that:

1. I have reviewed this Quarterly Report on Form 10-Q of Pressure BioSciences, Inc.;

2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;

3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

4. The registrant’s other certifying officer and I are responsible for establishing and maintaining disclosure controls and procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rule 13a-15(f) and 15d-15(f)) for the registrant and have:

a) Designed such disclosure controls and procedures, or caused such disclosure controls and procedures to be designed under our supervision, to ensure that material information relating to the registrant, including its consolidated subsidiary, is made known to us by others within those entities, particularly during the period in which this report is being prepared;

b) Designed such internal controls over financial reporting, or caused such internal controls over financial reporting to be designed under our supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for external purposes in accordance with generally accepted accounting principles;

c) Evaluated the effectiveness of the registrant’s disclosure controls and procedures and presented in this report our conclusions about the effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

d) Disclosed in this report any change in the registrant’s internal control over financial reporting that occurred during the registrant’s most recent fiscal quarter (the registrant’s fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely to materially affect, the registrant’s internal control over financial reporting; and

5. The registrant’s other certifying officer and I have disclosed, based on our most recent evaluation of internal control over financial reporting, to the registrant’s auditors and the audit committee of the registrant’s board of directors (or persons performing the equivalent functions):

a) All significant deficiencies and material weaknesses in the design or operation of internal control over financial reporting which are reasonably likely to adversely affect the registrant’s ability to record, process, summarize and report financial information; and

b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant’s internal control over financial reporting.

Dated: August 18, 2021

/s/ Richard T. Schumacher

Richard T. Schumacher

President and Chief Executive Officer

Principal Executive Officer

EX-31.2

EXHIBIT 31.2

CERTIFICATION PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

I, Richard T. Schumacher, certify that:

1. I have reviewed this Quarterly Report on Form 10-Q of Pressure BioSciences, Inc.;

2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;

3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

4. The registrant’s other certifying officer and I are responsible for establishing and maintaining disclosure controls and procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rule 13a-15(f) and 15d-15(f)) for the registrant and have:

a) Designed such disclosure controls and procedures, or caused such disclosure controls and procedures to be designed under our supervision, to ensure that material information relating to the registrant, including its consolidated subsidiary, is made known to us by others within those entities, particularly during the period in which this report is being prepared;

b) Designed such internal controls over financial reporting, or caused such internal controls over financial reporting to be designed under our supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for external purposes in accordance with generally accepted accounting principles;

c) Evaluated the effectiveness of the registrant’s disclosure controls and procedures and presented in this report our conclusions about the effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

d) Disclosed in this report any change in the registrant’s internal control over financial reporting that occurred during the registrant’s most recent fiscal quarter (the registrant’s fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely to materially affect, the registrant’s internal control over financial reporting; and

5. The registrant’s other certifying officer and I have disclosed, based on our most recent evaluation of internal control over financial reporting, to the registrant’s auditors and the audit committee of the registrant’s board of directors (or persons performing the equivalent functions):

a) All significant deficiencies and material weaknesses in the design or operation of internal control over financial reporting which are reasonably likely to adversely affect the registrant’s ability to record, process, summarize and report financial information; and

b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant’s internal control over financial reporting.

Dated: August 18, 2021

/s/ Richard T. Schumacher

Richard T. Schumacher

Principal Financial Officer

EX-32.1

EXHIBIT 32.1

Certification

Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

(Subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code)

In connection with the Quarterly Report on Form 10-Q of Pressure BioSciences, Inc., a Massachusetts corporation (the “Company”) for the period ended June 30, 2021 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Richard T. Schumacher, President and Chief Executive Officer of the Company, do hereby certify, pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 (subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code) that:

(1) The Report of the Company fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934, as amended; and

(2) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.

Date: August 18, 2021	By:	/s/ Richard T. Schumacher
		Richard T. Schumacher
		President & Chief Executive Officer
		(Principal Executive Officer)

A signed original of this written statement required by Section 906 has been provided to Pressure BioSciences, Inc. and will be retained by Pressure BioSciences, Inc. and furnished to the Securities and Exchange Commission or its staff upon request.

EX-32.2

EXHIBIT 32.2

Certification

Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

(Subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code)

In connection with the Quarterly Report on Form 10-Q of Pressure BioSciences, Inc., a Massachusetts corporation (the “Company”) for the period ended June 30, 2021 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), I, Richard T. Schumacher, Principal Financial Officer of the Company, do hereby certify, pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 (subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code) that:

(1) The Report of the Company fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934, as amended; and

(2) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.

Date: August 18, 2021	By:	/s/ Richard T. Schumacher
		Richard T. Schumacher
		President & Chief Executive Officer
		(Principal Financial Officer)

A signed original of this written statement required by Section 906 has been provided to Pressure BioSciences, Inc. and will be retained by Pressure BioSciences, Inc. and furnished to the Securities and Exchange Commission or its staff upon request.

XML

Cover - shares	6 Months Ended
	Jun. 30, 2021	Aug. 11, 2021
Cover [Abstract]
Document Type	10-Q
Amendment Flag	false
Document Quarterly Report	true
Document Transition Report	false
Document Period End Date	Jun. 30, 2021
Document Fiscal Period Focus	Q2
Document Fiscal Year Focus	2021
Current Fiscal Year End Date	--12-31
Entity File Number	001-38185
Entity Registrant Name	PRESSURE BIOSCIENCES, INC.
Entity Central Index Key	0000830656
Entity Tax Identification Number	04-2652826
Entity Incorporation, State or Country Code	MA
Entity Address, Address Line One	14 Norfolk Avenue
Entity Address, City or Town	South Easton
Entity Address, State or Province	MA
Entity Address, Postal Zip Code	02375
City Area Code	(508)
Local Phone Number	230-1828
Entity Current Reporting Status	Yes
Entity Interactive Data Current	Yes
Entity Filer Category	Non-accelerated Filer
Entity Small Business	true
Entity Emerging Growth Company	false
Entity Shell Company	false
Entity Common Stock, Shares Outstanding		6,371,190

XML

Consolidated Balance Sheets (Unaudited) - USD ($)	Jun. 30, 2021	Dec. 31, 2020
CURRENT ASSETS
Cash and cash equivalents	$ 46,625	$ 18,540
Accounts receivable	629,766	131,228
Inventories, net of $342,496 reserve at June 30, 2021 and December 31, 2020	492,095	592,767
Prepaid expenses and other current assets	219,161	314,936
Total current assets	1,387,647	1,057,471
Investment in equity securities	274,621	517,001
Property and equipment, net	7,431	16,490
Right of use asset leases	189,808	221,432
Intangible assets, net	447,115	490,385
TOTAL ASSETS	2,306,622	2,302,779
CURRENT LIABILITIES
Accounts payable	628,835	771,945
Accrued employee compensation	420,510	417,578
Accrued professional fees and other	2,163,563	2,037,806
Other current liabilities	8,165,281	6,330,722
Deferred revenue	49,189	47,328
Convertible debt, net of unamortized discounts of $2,353,152 and $3,948,167, respectively	10,909,314	7,545,670
Other debt, net of unamortized discounts of $5,495 and $0, respectively	1,363,931	1,135,469
Operating lease liability	69,204	65,193
Other related party debt	184,600	166,000
Total current liabilities	23,954,427	18,517,711
LONG TERM LIABILITIES
Long term debt	527,038	527,039
Operating lease liability – long term	120,604	156,239
Deferred revenue	7,446	19,382
TOTAL LIABILITIES	24,609,515	19,220,371
STOCKHOLDERS’ DEFICIT
Common stock, $.01 par value; 100,000,000 shares authorized; 6,260,884 and 4,168,324 shares issued and outstanding on June 30, 2021 and December 31, 2020 respectively	62,608	41,683
Warrants to acquire common stock	30,911,495	29,192,471
Additional paid-in capital	55,317,862	50,312,968
Accumulated deficit	(108,595,951)	(96,465,807)
Total stockholders’ deficit	(22,302,893)	(16,917,592)
TOTAL LIABILITIES AND STOCKHOLDERS’ DEFICIT	2,306,622	2,302,779
Series D Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	3	3
Series G Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	806	806
Series H Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	100	100
Series H2 Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock
Series J Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	35	35
Series K Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	68	68
Series AA Convertible Preferred Stock [Member]
STOCKHOLDERS’ DEFICIT
Convertible Preferred Stock	$ 81	$ 81

XML

Consolidated Balance Sheets (Unaudited) (Parenthetical) - USD ($)	Jun. 30, 2021	Dec. 31, 2020
Inventory Valuation Reserves		$ 342,496
Debt Instrument, Unamortized Discount	$ 2,353,152	3,948,167
Other debt, unamortized discounts net.	$ 5,495	$ 0
Common Stock, Par or Stated Value Per Share		$ 0.01
Common Stock, Shares Authorized		100,000,000
Common Stock, Shares, Outstanding	6,260,884	4,168,324
Series D Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	850	850
Preferred Stock, Shares Outstanding		300
Preferred Stock, Liquidation Preference, Value		$ 300,000
Series G Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	240,000	240,000
Preferred Stock, Shares Outstanding		80,570
Series H Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	10,000	10,000
Preferred Stock, Shares Outstanding		10,000
Series H2 Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized		21
Preferred Stock, Shares Outstanding		21
Series J Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	6,250	6,250
Preferred Stock, Shares Outstanding		3,458
Series K Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	15,000	15,000
Preferred Stock, Shares Outstanding		6,880
Series AA Convertible Preferred Stock [Member]
Preferred Stock, Par or Stated Value Per Share		$ 0.01
Preferred Stock, Shares Authorized	10,000	10,000
Preferred Stock, Shares Outstanding	8,083	8,043

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Consolidated Statements of Operations (Unaudited) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020
Revenue:
Total revenue	$ 608,927	$ 268,154	$ 1,168,801	$ 522,027
Costs and expenses:
Cost of products and services	286,660	134,882	512,935	310,028
Research and development	256,507	294,602	556,450	560,292
Selling and marketing	92,813	162,098	186,141	351,214
General and administrative	619,286	1,007,215	1,634,716	2,026,225
Total operating costs and expenses	1,255,266	1,598,797	2,890,242	3,247,759
Operating loss	(646,339)	(1,330,643)	(1,721,441)	(2,725,732)
Other (expense) income:
Interest expense, net	(3,526,141)	(1,724,879)	(8,194,205)	(3,296,679)
Unrealized (loss) gain on investment in equity securities	(134,477)	196,891	(242,380)	346,262
Loss on extinguishment of liabilities	(498,226)	(1,710,151)	(1,223,385)	(2,846,518)
Other income	60,012		58,653
Total other expense	(4,098,832)	(3,238,139)	(9,601,317)	(5,796,935)
Net loss	(4,745,171)	(4,568,782)	(11,322,758)	(8,522,667)
Deemed dividends on beneficial conversion feature			(57,884)
Preferred stock dividends	(404,171)	(396,970)	(807,386)	(721,556)
Net loss attributable to common stockholders	$ (5,149,342)	$ (4,965,752)	$ (12,188,028)	$ (9,244,223)
Basic and diluted net loss per share attributable to common stockholders	$ (0.90)	$ (1.70)	$ (2.29)	$ (3.34)
Weighted average common stock shares outstanding used in the basic and diluted net loss per share calculation	5,748,711	2,914,659	5,312,172	2,765,132
Products, Services, Other [Member]
Revenue:
Total revenue	$ 608,927	$ 268,154	$ 1,168,801	$ 522,027

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Consolidated Statements of Cash Flows (Unaudited) - USD ($)	6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020
CASH FLOWS FROM OPERATING ACTIVITIES:
Net loss	$ (11,322,758)	$ (8,522,667)
Adjustments to reconcile net loss to net cash used in operating activities:
Gain on loan forgiveness	(367,039)
Non-cash lease expense	31,624	35,927
Common stock and warrants issued for interest and extension fees	4,054,749	159,784
Depreciation and amortization	56,291	74,247
Accretion of interest and amortization of debt discount	3,909,024	1,750,911
Loss on extinguishment of accrued liabilities and debt		977,622
Stock-based compensation expense	124,695	307,110
Loss (Gain) on investment in equity securities	242,380	(346,262)
Common stock issued for services	238,512	87,963
Changes in operating assets and liabilities:
Accounts receivable	(498,538)	108,096
Inventories	100,672	2,853
Prepaid expenses and other assets	95,775	20,944
Accounts payable	(143,110)	(5,927)
Accrued employee compensation	2,932	(58,775)
Operating lease liability	(31,624)	(35,927)
Deferred revenue and other accrued expenses	1,415,688	2,643,607
Net cash used in operating activities	(2,090,727)	(2,800,494)
CASH FLOWS FROM INVESTING ACTIVITIES:
Advance on loan receivable		(531,250)
Purchases of property plant and equipment	(3,962)	(1,663)
Net cash used in investing activities	(3,962)	(532,913)
CASH FLOWS FROM FINANCING ACTIVITIES:
Net proceeds from Series AA Convertible Preferred Stock	100,000
Proceeds from stock option exercises	14,773
Net proceeds from convertible debt	2,598,250	4,422,600
Net proceeds from non-convertible debt – third party	1,183,188	990,539
Net proceeds from non-convertible debt – related party	171,600	8,500
Payments on convertible debt	(1,200,996)	(1,257,250)
Payments on non-convertible debt – related party	(153,000)
Payments on non-convertible debt	(591,041)	(819,149)
Net cash provided by financing activities	2,122,774	3,345,240
NET INCREASE IN CASH AND CASH EQUIVALENTS	28,085	11,833
CASH AND CASH EQUIVALENTS AT BEGINNING OF YEAR	18,540	29,625
CASH AND CASH EQUIVALENTS AT END OF PERIOD	46,625	41,458
SUPPLEMENTAL INFORMATION
Interest paid in cash	383,403	419,817
NON CASH TRANSACTIONS:
Interest added to principal		152,552
Common stock issued to settle accrued liabilities		127,855
Common stock issued with debt	112,877
Discount from warrants issued with debt	1,068,842	2,958,693
Common stock issued in lieu of cash for dividend	114,298	176,748
Preferred stock dividends	807,386	721,556
Conversion of debt and interest into common stock	349,250	1,317,649
Discount due to beneficial conversion feature	566,847	982,097
Deemed dividend - beneficial conversion feature	$ 57,884

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Consolidated Statements of Changes in Stockholders' Deficit (Unaudited) - USD ($)	Series D Preferred Stock [Member]	Series G Preferred Stock [Member]	Series H Preferred Stock [Member]	Series H(2) Preferred Stock [Member]	Series J Preferred Stock [Member]	Series K Preferred Stock [Member]	Series AA Preferred Stock [Member]	Common Stock [Member]	Stock Warrants [Member]	Additional Paid-in Capital [Member]	Retained Earnings [Member]	Total
Balance at Dec. 31, 2019	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 25,496	$ 22,599,177	$ 44,261,105	$ (78,942,277)	$ (12,055,407)
Balance, shares at Dec. 31, 2019	300	80,570	10,000	21	3,458	6,880	7,939	2,549,620
Stock-based compensation										241,769		241,769
Series AA Preferred Stock dividend											(324,586)	(324,586)
Series AA preferred stock dividend, shares
Issuance of common stock to settle accrued liabilities								$ 665		127,190		127,855
Issuance of common stock to settle accrued liabilities, shares								66,500
Common stock issued for debt settlement								$ 100		24,900		25,000
Common stock issued for debt settlement, shares								10,000
Issuance of common stock for debt extension and interest paid in kind								$ 385		60,175		60,560
Issuance of common stock for debt extension and interest paid-in-kind, shares								38,521
Beneficial conversion feature on debt										404,608		404,608
Warrants issued with debt									1,205,010			1,205,010
Warrants issued for debt extension									609,143			609,143
Net loss											(3,953,885)	(3,953,885)
Balance at Mar. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 26,646	24,413,330	45,119,747	(83,220,748)	(13,659,933)
Balance, shares at Mar. 31, 2020	300	80,570	10,000	21	3,458	6,880	7,939	2,664,641
Balance at Dec. 31, 2019	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 25,496	22,599,177	44,261,105	(78,942,277)	(12,055,407)
Balance, shares at Dec. 31, 2019	300	80,570	10,000	21	3,458	6,880	7,939	2,549,620
Issuance of common stock for services												$ 87,963
Issuance of common stock for services, shares												25,000
Conversion of debt and interest for common stock												$ 1,317,649
Conversion of debt and interest for common stock, shares												420,746
Net loss												$ (8,522,667)
Balance at Jun. 30, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 32,072	26,527,615	47,413,735	(88,186,500)	(14,211,986)
Balance, shares at Jun. 30, 2020	300	80,570	10,000	21	3,458	6,880	7,939	3,207,285
Balance at Mar. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 26,646	24,413,330	45,119,747	(83,220,748)	(13,659,933)
Balance, shares at Mar. 31, 2020	300	80,570	10,000	21	3,458	6,880	7,939	2,664,641
Stock-based compensation										65,341		65,341
Series AA Preferred Stock dividend											(396,970)	(396,970)
Series AA preferred stock dividend, shares
Issuance of common stock for services								$ 250		87,713		87,963
Issuance of common stock for services, shares								25,000
Beneficial conversion feature on debt										577,489		577,489
Warrants issued with debt									1,753,683			1,753,683
Warrants issued for debt extension									360,602			360,602
Conversion of debt and interest for Common Stock								$ 4,107		1,288,542		1,292,649
Common stock issued for extinguishment of liabilities, shares								410,746
Issuance of common stock for interest paid in kind								$ 425		98,799		99,224
Issuance of common stock for debt extension and interest paid-in-kind, shares								42,510
Issuance of common stock for dividends paid-in-kind								$ 644		176,104		176,748
Net loss											(4,568,782)	(4,568,782)
Issuance of common stock for dividends paid-in-kind, shares								64,388
Balance at Jun. 30, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80	$ 32,072	26,527,615	47,413,735	(88,186,500)	(14,211,986)
Balance, shares at Jun. 30, 2020	300	80,570	10,000	21	3,458	6,880	7,939	3,207,285
Balance at Dec. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 41,683	29,192,471	50,312,968	(96,465,807)	(16,917,592)
Balance, shares at Dec. 31, 2020	300	80,570	10,000	21	3,458	6,880	8,043	4,168,324
Stock-based compensation										61,237		61,237
Stock option exercise								$ 214		14,559		14,773
Stock option exercise, shares								21,411
Series AA Preferred Stock dividend											(403,215)	(403,215)
Series AA preferred stock dividend, shares
Issuance of common stock for services								$ 1,124		237,388		238,512
Issuance of common stock for services, shares								112,400
Issuance of warrants for interest paid-in-kind									600,298			600,298
Beneficial conversion feature on debt										53,777		53,777
Warrants issued with debt									162,654			162,654
Series AA Preferred Stock offering									49,884	50,116		100,000
Series AA Preferred Stock offering, shares							40
Beneficial conversion option on convertible preferred stock										57,884		57,884
Deemed dividend on convertible preferred stock										(57,884)		(57,884)
Deemed dividend on convertible preferred stock, shares
Conversion of debt and interest for common stock								$ 472		117,528		118,000
Conversion of debt and interest for common stock, shares								47,200
Issuance of common stock for interest paid in kind								$ 9,224		2,012,556		2,021,780
Issuance of common stock for debt extension and interest paid-in-kind, shares								922,372
Net loss											(6,577,587)	(6,577,587)
Balance at Mar. 31, 2021	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 52,717	30,005,307	52,860,129	(103,446,609)	(20,527,363)
Balance, shares at Mar. 31, 2021	300	80,570	10,000	21	3,458	6,880	8,083	5,271,707
Balance at Dec. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 41,683	29,192,471	50,312,968	(96,465,807)	$ (16,917,592)
Balance, shares at Dec. 31, 2020	300	80,570	10,000	21	3,458	6,880	8,043	4,168,324
Stock option exercise, shares												21,411
Common stock issued for debt settlement, shares								120,000				56,067
Issuance of common stock for services												$ 238,512
Issuance of common stock for services, shares												112,400
Conversion of debt and interest for common stock												$ 349,250
Conversion of debt and interest for common stock, shares												139,700
Issuance of common stock for interest paid in kind												$ 3,500,000
Net loss												(11,322,758)
Balance at Jun. 30, 2021	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 62,608	30,911,495	55,317,862	(108,595,951)	(22,302,893)
Balance, shares at Jun. 30, 2021	300	80,570	10,000	21	3,458	6,880	8,083	6,260,884
Balance at Mar. 31, 2021	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 52,717	30,005,307	52,860,129	(103,446,609)	(20,527,363)
Balance, shares at Mar. 31, 2021	300	80,570	10,000	21	3,458	6,880	8,083	5,271,707
Stock-based compensation										63,458		63,458
Series AA Preferred Stock dividend											(404,171)	(404,171)
Series AA preferred stock dividend, shares
Common stock issued for debt settlement, shares								665,000
Issuance of common stock for debt extension and interest paid-in-kind, shares
Beneficial conversion feature on debt										513,070		513,070
Warrants issued with debt									906,188			906,188
Conversion of debt and interest for common stock								$ 925		230,325		231,250
Conversion of debt and interest for common stock, shares								92,500
Issuance of common stock for interest paid in kind								$ 7,206		1,425,465		1,432,671
Issuance of common stock for debt extension and interest paid-in-kind, shares								720,610
Issuance of common stock for dividends paid-in-kind								$ 560		113,738		114,298
Stock issued with debt								$ 1,200		111,677		112,877
Stock issued with debt, shares								120,000
Net loss											(4,745,171)	(4,745,171)
Issuance of common stock for dividends paid-in-kind, shares								56,067
Balance at Jun. 30, 2021	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 62,608	$ 30,911,495	$ 55,317,862	$ (108,595,951)	$ (22,302,893)
Balance, shares at Jun. 30, 2021	300	80,570	10,000	21	3,458	6,880	8,083	6,260,884

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Business Overview, Liquidity and Management Plans

6 Months Ended

Jun. 30, 2021

Organization, Consolidation and Presentation of Financial Statements [Abstract]

Business Overview, Liquidity and Management Plans

1) Business Overview, Liquidity and Management Plans

Pressure BioSciences, Inc. (“we”, “our”, “the Company”) develops and sells innovative, broadly enabling, pressure-based platform solutions for the worldwide life sciences industry. Our solutions are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions (e.g., cell lysis, biomolecule extraction) safely and reproducibly. Our primary focus has historically been in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technology expertise in two new platform technology areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. for gently controlled disaggregation and refolding of biotherapeutic proteins (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies.

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Going Concern

6 Months Ended

Jun. 30, 2021

Organization, Consolidation and Presentation of Financial Statements [Abstract]

Going Concern

2) Going Concern

The accompanying financial statements have been prepared assuming that the Company will continue as a going concern, which contemplates the realization of assets and the liquidation of liabilities in the normal course of business. However, we have experienced losses from operations and negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of June 30, 2021, we do not have adequate working capital resources to satisfy our current liabilities and as a result, there is substantial doubt regarding our ability to continue as a going concern. We have been successful in raising debt and equity capital in the past and as described in Notes 5 and 6. In addition we raised debt and equity capital after June 30, 2021 as described in Note 7. We have financing efforts in place to continue to raise cash through debt and equity offerings. Although we have successfully completed financings and reduced expenses in the past, we cannot assure you that our plans to address these matters in the future will be successful. These financial statements do not include any adjustments that might result from this uncertainty.

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Summary of Significant Accounting Policies

6 Months Ended

Jun. 30, 2021

Accounting Policies [Abstract]

Summary of Significant Accounting Policies

3) Summary of Significant Accounting Policies

Basis of Presentation

The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2020.

Use of Estimates

The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements.

Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiary PBI BioSeq, Inc. All intercompany accounts and transactions have been eliminated in consolidation.

Revenue Recognition

We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, and ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers. Revenue is measured based on a consideration specified in a contract with a customer, and excludes any sales incentives and amounts collected on behalf of third parties. We enter into sales contracts that may consist of multiple distinct performance obligations where certain performance obligations of the sales contract are not delivered in one reporting period. We measure and allocate revenue according to ASC 606-10.

We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation.

Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue.

Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods.

Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue.

The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components.

Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements.

We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

a)	The fair value of the asset or service involved is not determinable.
b)	The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange.
c)	The transaction lacks commercial substance.

We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold.

We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases.

Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract.

Disaggregation of revenue

In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition.

In thousands of US dollars ($)	Three Months Ended June 30,				Six Months Ended June 30,
Primary geographical markets	2021		2020		2021		2020
North America	$	477	$	162	$	685	$	307
Europe		103		3		187		4
Asia		29		103		297		211
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Major products/services lines		2021		2020		2021		2020
Hardware	$	336	$	122	$	713	$	217
Consumables		44		51		146		107
Contract research services		136		34		142		44
Sample preparation accessories		40		33		69		58
Technical support/extended service contracts		34		17		58		36
Shipping and handling		16		6		35		15
Other		3		5		6		45
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Timing of revenue recognition		2021		2020		2021		2020
Products transferred at a point in time	$	440	$	217	$	969	$	442
Services transferred over time		169		51		200		80
	$	609	$	268	$	1,169	$	522

Contract balances

In thousands of US dollars ($)	June 30, 2021		December 31, 2020
Receivables, which are included in ‘Accounts Receivable’	$	630	$	131
Contract liabilities (deferred revenue)		57		67

Transaction price allocated to the remaining performance obligations.

The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period.

 Schedule of Future Related to Performance Obligations

In thousands of US dollars ($)	2021		2022		Total
Extended warranty service	$	49		8	$	57

All consideration from contracts with customers is included in the amounts presented above.

Contract Costs

The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing.

Concentrations

Credit Risk

Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories.

The following table illustrates the level of concentration as a percentage of total revenues during the three and six months ended June 30, 2021 and 2020.

	For the Three Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		71	%
Federal Agencies		14	%		2	%

	For the Six Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		70	%
Federal Agencies		8	%		4	%

The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of June 30, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable.

	June 30, 2021			December 31, 2020
Top Five Customers		56	%		89	%
Federal Agencies		11	%		10	%

Product Supply

We utilize a contract assembler for our Barocycler® 2320EXT. They provide us with precision manufacturing services that include management support services to meet our specific application and operational requirements. Among the services provided to us are:

● CNC Machining

● Contract Assembly & Kitting

● Component and Subassembly Design

● Inventory Management

● ISO certification

At this time, we believe that outsourcing contract assembly of our Barocycler® 2320EXT is the most cost-effective method for us to obtain ISO Certified, CE and CSA Marked instruments. The Company also continues to evaluate the potential economic benefits of bringing instrument assembly in-house.

We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments.

Investment in Equity Securities

As of June 30, 2021, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company).

We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income.

As of June 30, 2021, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $274,621. We recorded $242,380 as an unrealized loss during the six months ended June 30, 2021 for changes in market value.

Computation of Loss per Share

Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss.

The following table illustrates our computation of loss per share for the three and six months ended June 30, 2021 and 2020:

	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2021			2020			2021			2020
Numerator:
Net loss attributable to common stockholders	$	(5,149,342	)	$	(4,965,752	)	$	(12,188,028	)	$	(9,244,223	)
Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding		5,748,711			2,914,659			5,312,172			2,765,132
Loss per common share – basic and diluted	$	(0.90	)	$	(1.70	)	$	(2.29	)	$	(3.34	)

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms.

	As of June 30,
	2021		2020
Stock options		1,350,046		1,392,370
Convertible debt		5,083,187		3,905,867
Common stock warrants		15,703,807		12,761,126
Convertible preferred stock:
Series D Convertible Preferred Stock		25,000		25,000
Series G Convertible Preferred Stock		26,857		26,857
Series H Convertible Preferred Stock		33,334		33,334
Series H2 Convertible Preferred Stock		70,000		70,000
Series J Convertible Preferred Stock		115,267		115,267
Series K Convertible Preferred Stock		229,334		229,334
Series AA Convertible Preferred Stock		8,083,000		7,939,000
		30,719,832		26,498,155

Accounting for Stock-Based Compensation Expense

We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant.

Determining Fair Value of Stock Option Grants

Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period.

Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted.

Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award.

Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term.

Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense.

The Company recognized stock-based compensation expense of $63,458 and $65,341 for the three months ended June 30, 2021 and 2020, respectively. The Company recognized stock-based compensation expense of $124,695 and $307,110 for the six months ended June 30, 2021 and 2020, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations:

	For the Three Months Ended				For the Six Months Ended
	June 30,				June 30,
	2021		2020		2021		2020
Cost of sales	$	5,107	$	5,107	$	10,160	$	13,063
Research and development		26,491		26,137		52,353		64,963
Selling and marketing		5,887		6,358		10,482		20,294
General and administrative		25,973		27,739		51,700		208,790
Total stock-based compensation expense	$	63,458	$	65,341	$	124,695	$	307,110

Fair Value of Financial Instruments

Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. Long-term liabilities include debt and deferred revenue with a carrying value that approximates fair value.

Fair Value Measurements

The Company follows the guidance of FASB ASC Topic 820, “Fair Value Measurements and Disclosures” (“ASC 820”) as it related to all financial assets and financial liabilities that are recognized or disclosed at fair value in the financial statements on a recurring basis.

The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement.

Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management.

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of June 30, 2021:

			Fair value measurements at June 30, 2021 using:
	June 30, 2021		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities	$	274,621	$	274,621		-		-
Total Financial Assets	$	274,621	$	274,621	$	-	$	-

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020:

			Fair value measurements at December 31, 2020 using:
	December 31, 2020		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities		517,001		517,001		-		-
Total Financial Assets	$	517,001	$	517,001	$	-	$	-

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Commitments and Contingencies

6 Months Ended

Jun. 30, 2021

Commitments and Contingencies Disclosure [Abstract]

Commitments and Contingencies

4) Commitments and Contingencies

Operating Leases

The Company accounts for its leases under ASC 842. The Company has elected to apply the short-term lease exception to leases of one year or less.

Our corporate office is currently located at 14 Norfolk Avenue, South Easton, Massachusetts 02375. We are currently paying $6,950 per month, on a lease extension, signed on December 30, 2020, that expires December 31, 2021, for our corporate office. We expanded our space to include offices, warehouse, and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current payments.

We extended our lease for our space in Medford, MA (the “Medford Lease”) from December 30, 2020 to December 30, 2023. The lease requires monthly payments of $7,282 subject to annual cost of living increases. The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.

The Company accounted for the lease extension of our Medford Lease as a lease modification under ASC 842. At the effective date of modification, the Company recorded an adjustment to the right-of-use asset and lease liability in the amount of $221,432 based on the net present value of lease payments discounted using an estimated borrowing rate of 12%.

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms as of June 30, 2021:

2021	$	85,391
2022		87,383
2023		87,383
Total minimum payments required	$	260,157

Target Discovery, Inc.

In the six months ended June 30, 2021, the Company incurred $34,400 in fees with Target Discovery, Inc. for the use of their facilities and other services.

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Convertible Debt and Other Debt

6 Months Ended

Jun. 30, 2021

Debt Disclosure [Abstract]

Convertible Debt and Other Debt

5) Convertible Debt and Other Debt

Convertible Debt

On various dates during the six months ended June 30, 2021, the Company issued convertible notes for a total of $3,104,625 which contained varied terms and conditions as follows: a) 6-12 month maturity date; b) interest rates of 10-18%; c) convertible to the Company’s common stock at issuance at a fixed rate of $2.50 or at variable conversion rates upon an event of default. These notes were issued with either shares of common stock or warrants to purchase common stock that were fair valued at issuance date. The aggregate relative fair value of the shares of common stock and warrants issued with the notes of $1,181,719 was recorded as a debt discount to be amortized over the term of the notes. We then computed the effective conversion price of the notes, and recorded a $566,847 beneficial conversion feature as a debt discount to be amortized over the term of the notes. We also evaluated the convertible notes for derivative liability treatment and determined that the notes did not qualify for derivative accounting treatment at June 30, 2021.

The specific terms of the convertible notes and outstanding balances as of June 30, 2021 are listed in the tables below.

Inception Date		Term		Loan Amount		Outstanding balance with OID		Original Issue Discount (OID)		Interest Rate			Conversion Price		Deferred Finance Fees		Discount for conversion feature and warrants/shares
May 17, 2018 (1) (2)			12 months	$	380,000	$	166,703	$	15,200		8	%	$	2.50	$	15,200	$	332,407
June 8, 2018 (1) (4)	(1)		6 months	$	50,000	$	50,000	$	2,500		2	%	$	7.50	$	2,500	$	3,271
October 19, 2018 (1)			6 months	$	100,000	$	100,000	$	-		5	%	$	7.50	$	-	$	-
November 13, 2018 (1) (3) (4)			6 months	$	200,000	$	78,508	$	-		5	%	$	2.50	$	-	$	168,634
January 3, 2019 (1) (4)			6 months	$	50,000	$	50,000	$	2,500		24	%	$	7.50	$	2,500	$	-
February 21, 2019 (1) (2)			12 months	$	215,000	$	215,000	$	-		4	%	$	2.50	$	15,000	$	107,709
March 18, 2019 (1)			6 months	$	100,000	$	100,000	$	-		4	%	$	7.50	$	-	$	10,762
June 4, 2019 (1) (2)			9 months	$	500,000	$	302,484	$	-		8	%	$	2.50	$	40,500	$	70,631
June 19, 2019 (1) (2)			12 months	$	105,000	$	105,000	$	-		4	%	$	2.50	$	5,000	$	2,646
June 7, 2019 (1) (4)			6 months	$	125,000	$	6,746	$	-		5	%	$	7.50	$	-	$	18,254
July 1, 2019 (1) (2)			12 months	$	107,500	$	107,500	$	-		4	%	$	2.50	$	7,500	$	85,791
July 19, 2019 (1) (2)			12 months	$	115,000	$	115,000	$	-		4	%	$	2.50	$	5,750	$	15,460
July 19, 2019 (1) (2)			12 months	$	130,000	$	130,000	$	-		6	%	$	2.50	$	6,500	$	-
September 27, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	13,759
October 24, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	-
November 1, 2019 (1) (2)			12 months	$	270,000	$	270,000	$	-		6	%	$	2.50	$	13,500	$	-
November 15, 2019 (1)			12 months	$	385,000	$	320,000	$	35,000		10	%	$	2.50	$	35,000	$	90,917
January 2, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	91,606
January 24, 2020 (1)			12 months	$	247,500	$	247,500	$	22,500		10	%	$	2.50	$	22,500	$	89,707
January 29, 2020 (1)			12 months	$	363,000	$	363,000	$	33,000		10	%	$	2.50	$	33,000	$	297,000
February 12, 2020 (1)			12 months	$	275,000	$	275,000	$	25,000		10	%	$	2.50	$	25,000	$	225,000
February 19, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	135,000
March 11, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	232,810
March 13, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	60,705
March 26, 2020 (1)			12 months	$	111,100	$	111,100	$	10,100		10	%	$	2.50	$	10,100	$	90,900
April 8, 2020 (1)			12 months	$	276,100	$	276,100	$	25,100		10	%	$	2.50	$	25,000	$	221,654
April 17, 2020 (1)			12 months	$	143,750	$	143,750	$	18,750		10	%	$	2.50	$	-	$	96,208
April 30, 2020 (1)			12 months	$	546,250	$	546,250	$	71,250		10	%	$	2.50	$	47,500	$	427,500
May 6, 2020 (1)			12 months	$	460,000	$	460,000	$	60,000		10	%	$	2.50	$	40,000	$	360,000
May 18, 2020 (1)			12 months	$	546,250	$	221,250	$	46,250		10	%	$	2.50	$	35,500	$	439,500
June 2, 2020 (1)			12 months	$	902,750	$	652,750	$	92,750		10	%	$	2.50	$	58,900	$	708,500
June 12, 2020 (1)			12 months	$	57,500	$	57,500	$	7,500		10	%	$	2.50	$	5,000	$	45,000
June 22, 2020 (1)			12 months	$	138,000	$	138,000	$	18,000		10	%	$	2.50	$	12,000	$	108,000
July 7, 2020 (1)			12 months	$	586,500	$	586,500	$	76,500		10	%	$	2.50	$	51,000	$	400,234
July 17, 2020 (1)			12 months	$	362,250	$	362,250	$	47,250		10	%	$	2.50	$	31,500	$	185,698
July 29, 2020 (1)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	241,245
July 21, 2020 (1) (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	24,875
August 14, 2020 (1)			12 months	$	762,450	$	462,450	$	69,450		10	%	$	2.50	$	66,300	$	580,124
September 10, 2020			12 months	$	391,000	$	391,000	$	51,000		10	%	$	2.50	$	34,000	$	231,043
September 21, 2020 (5)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	66,375
September 23, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	20,500
September 25, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	-	$	19,125
December 3, 2020			12 months	$	299,000	$	299,000	$	39,000		10	%	$	2.50	$	26,000	$	197,882
October 22, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	18,875
December 23, 2020 (1) (5)			6 months	$	1,000,000	$	150,000	$	100,000		10	%	$	2.50	$	-	$	833,536
January 5, 2021 (1)			6 months	$	575,000	$	575,000	$	75,000		18	%	$	2.50	$	-	$	-
February 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	180,000
March 23, 2021			12 months	$	55,000	$	55,000	$	5,000		10	%	$	2.50	$	-	$	36,431
May 24, 2021			6 months	$	54,625	$	54,625	$	7,125		12	%	$	2.50	$	-	$	-
May 6, 2021			12 months	$	402,500	$	402,500	$	52,500		10	%	$	2.50	$	35,000	$	312,551
June 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	144,760
June 25, 2021			12 months	$	977,500	$	977,500	$	127,500		10	%	$	2.50	$	-	$	773,802
May 20, 2021			12 months	$	180,000	$	180,000	$	30,000		10	%	$	2.50	$	15,000	$	25,824
June 3, 2021			12 months	$	50,000	$	50,000	$	1,500		12	%	$	2.50	$	-	$	7,948
June 28, 2021			12 months	$	350,000	$	350,000	$	35,000		12	%		(6)	$	22,750	$	267,250
						$	13,262,466	$	1,502,225						$	972,500	$	9,117,409

(1)	The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.
(2)	The Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below).
(3)	Interest was capitalized and added to outstanding principal.
(4)	During the year ended December 31, 2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.
(5)	The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.
(6)	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.

As of June 30, 2021 one lender holds approximately $9.1 million of the $13.3 million convertible notes outstanding.

For the six months ended June 30, 2021, the Company recognized amortization expense related to the debt discounts indicated above of $3,864,956. The unamortized debt discounts as of June 30, 2021 related to the convertible debentures and other convertible notes amounted to $2,353,152.

Standstill and Forbearance Agreements

The Company has entered into Standstill and Forbearance Agreements with lenders who hold convertible notes with a total principal as of June 30, 2021 of $1.57 million. Pursuant to the Standstill and Forbearance Agreements, the lenders agreed to not convert any portion of their notes into shares of common stock at a variable rate until March 31, 2021 for convertible notes with a principal balance of $469 thousand and until April 16, 2021 for convertible notes with a principal balance of $1.1 million. For the six months ended June 30, 2021, the Company incurred fees of approximately $1.0 million in connection with these agreements. After June 30, 2021 the Company settled $827,500 of these loans (see Note 7).

Convertible Loan Modifications and Extinguishments

We refinanced certain convertible loans during the three months ended June 30, 2021 at substantially the same terms for extensions ranging over a period of three to six months. We amortized any remaining unamortized debt discount as of the modification date over the remaining, extended term of the new loans. We applied ASC 470 of modification accounting to the debt instruments which were modified during the quarter or those settled with new notes issued concurrently for the same amounts but different maturity dates. The terms such as the interest rate, prepayment penalties, and default rates will be the same over the new extensions. According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications.

The cash flows of new debt exceeded 10% of the remaining cash flows of the original debt on several loans. During the six months ended June 30, 2021 we recorded losses on extinguishment of liabilities of approximately $1.6 million by calculating the difference of the fair value of the new debt and the carrying value of the old debt.

The following table provides a summary of the changes in convertible debt, net of unamortized discounts, during 2021:

	2021
Balance at January 1,	$	7,545,670
Issuance of convertible debt, face value		3,104,625
Deferred financing cost		(506,375	)
Beneficial conversion feature on convertible note		(566,847	)
Debt discount from shares and warrants issued with debt		(1,181,719	)
Payments		(1,200,996	)
Conversion of debt into equity		(150,000	)
Accretion of interest and amortization of debt discount to interest expense		3,864,956
Balance at June 30,		10,909,314
Less: current portion		10,909,314
Convertible debt, long-term portion	$	–

Other Notes

On September 9, 2019 and February 28, 2020 we received a total of $966,500 unsecured non-convertible loans from a private investor with a one-month term. During the year ended December 31, 2020, the Company received net proceeds of $463,500, issued 150,000 warrants to purchase common stock (five-year term and $3.50 exercise price) and repaid $275,000. The relative fair value of $185,660 of the warrants issued with the note was recorded as a debt discount to be amortized over the term of the notes. As of June 30, 2021, the Company owes $691,500 on these notes which are past due. The Company and the investor are negotiating in good faith to extend the loans.

On October 1, 2019, the Company and the holder of the $170,000 non-convertible loan issued in May 2017 agreed to extend the term of the loan to December 31, 2019. The Company agreed to issue 1,200 shares of its common stock per month while the note remains outstanding. The note will continue to earn 10% annual interest. The loan is currently past due, and the Company and the investor are negotiating in good faith to extend the loan.

On October 11, 2019 we received a non-convertible loan with a one-month term and a 2% interest charge for $25,000 from a private investor. The loan is past due, and the Company and the investor are negotiating in good faith to extend the loan.

Merchant Agreements

We have signed various Merchant Agreements which are secured by second position rights to all customer receipts until the loan has been repaid in full and subject to interest rates of 9.3% - 11.5% per month. As illustrated in the following table, under the terms of these agreements, we received the disclosed Purchase Price and agreed to repay the disclosed Purchase Amount, which is collected by the Merchant lenders at the disclosed Daily Payment Rate. The Company’s Chief Executive Officer is personally guaranteeing the performance of the merchant loans.

The following table shows our Merchant Agreements as of June 30, 2021:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
February 4, 2021		125,000		165,000		55,281		1,032		-
March 11, 2021		125,000		167,500		40,559		1,396		2,500
March 26, 2021		240,000		330,960		129,952		2,364		-
May 3, 2021		200,000		275,800		141,458		1,970		5,000
June 2, 2021		135,000		186,165		115,677		1,400		1,350
	$	825,000	$	1,125,425	$	482,927	$	8,162	$	8,850

The following table shows our Merchant Agreements as of December 31, 2020:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
November 5, 2020	$	200,000	$	275,800	$	163,955		1,724	$	-
November 19, 2020		100,000		137,900		85,013		985		-
	$	300,000	$	413,700	$	248,968	$	2,709	$	-

We have accounted for the Merchant Agreements as loans under ASC 860 because while we provided rights to current and future receipts, we still had control over the receipts. The difference between the Purchase Amount and the Purchase Price is imputed interest that is recorded as interest expense when paid each day.

Related Party Notes

In June 2018, we received a non-convertible loan of $15,000 from a private investor. The loan includes a one-year term and 15% guaranteed interest. This loan remains outstanding at June 30, 2021 and is currently past due.

During the six months ended June 30, 2021, we received short-term non-convertible loans of $171,600 from related parties and repaid $153,000 of related party loans. These notes bear interest ranging from 10% to 15% and are due upon demand.

Long term debt

During the six months ended June 30, 2021, the Company borrowed $367,038 through a COVID-19 program that was sponsored by the United States and administered by the Small Business Administration (the “SBA”). The most notable programs were the Payroll Protection Program (or “PPP”) and the Economic Injury Disaster Loan program (or “EIDL”). PPP loan has a 1% interest rate and a five-year term. During this period, the Company’s first PPP loan borrowed in 2020 ($367,039) was forgiven by the SBA. This gain was reported in losses on extinguishment of liabilities on the consolidated statements of operations.

The Company’s EIDL loan, $150,000, accrues interest at 3.75% and requires monthly payments of $731 for principal and interest beginning in June 2021. The balance of the principal will be due in 30 years. In connection with the EIDL loan the Company entered into a security agreement with the SBA, whereby the Company granted the SBA a security interest in all of the Company’s right, title and interest in all of the Company’s assets.

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Stockholders’ Deficit

6 Months Ended

Jun. 30, 2021

Equity [Abstract]

Stockholders’ Deficit

  6) Stockholders’ Deficit

Preferred Stock

We are authorized to issue 1,000,000 shares of preferred stock with a par value of $0.01. Of the 1,000,000 shares of preferred stock:

1)	20,000 shares have been designated as Series A Junior Participating Preferred Stock (“Junior A”)
2)	313,960 shares have been designated as Series A Convertible Preferred Stock (“Series A”)
3)	279,256 shares have been designated as Series B Convertible Preferred Stock (“Series B”)
4)	88,098 shares have been designated as Series C Convertible Preferred Stock (“Series C”)
5)	850 shares have been designated as Series D Convertible Preferred Stock (“Series D”)
6)	500 shares have been designated as Series E Convertible Preferred Stock (“Series E”)
7)	240,000 shares have been designated as Series G Convertible Preferred Stock (“Series G”)
8)	10,000 shares have been designated as Series H Convertible Preferred Stock (“Series H”)
9)	21 shares have been designated as Series H2 Convertible Preferred Stock (“Series H2”)
10)	6,250 shares have been designated as Series J Convertible Preferred Stock (“Series J”)
11)	15,000 shares have been designated as Series K Convertible Preferred Stock (“Series K”)
12)	10,000 shares have been designated as Series AA Convertible Preferred Stock (“Series AA”)

As of June 30, 2021, there were no shares of Junior A, and Series A, B, C and E issued and outstanding. See our Annual Report on Form 10-K for the year ended December 31, 2020 for the pertinent disclosures of preferred stock.

During the six months ended June 30, 2021, the Company entered into Securities Purchase Agreements with accredited investors pursuant to which the Company sold an aggregate of 40 shares of Series AA Convertible Preferred Stock, each preferred share convertible into 1,000 shares of the Company’s common stock, par value $0.01 per share, for an aggregate Purchase price of $100,000. We issued to the investors warrants to purchase an aggregate 40,000 shares of common stock with an exercise price of $3.50 per share. The Company did not incur any placement agent fees for this transaction.

Stock Options and Warrants

At the Company’s December 12, 2013 Special Meeting, the shareholders approved the 2013 Equity Incentive Plan (the “2013 Plan”) pursuant to which 3,000,000 shares of our common stock were reserved for issuance upon exercise of stock options or other equity awards. Under the 2013 Plan, we may award stock options, shares of common stock, and other equity interests in the Company to employees, officers, directors, consultants, and advisors, and to any other persons the Board of Directors deems appropriate. As of June 30, 2021, options to acquire 1,350,046 shares were outstanding under the Plan.

As of June 30, 2021, total unrecognized compensation cost related to the unvested stock-based awards was $218,339, which is expected to be recognized over weighted average period of 1.62 years. The aggregate intrinsic value associated with the options outstanding and exercisable, and the aggregate intrinsic value associated with the warrants outstanding and exercisable as of June 30, 2021, based on the June 30, 2021 closing stock price of $2.17, was $1,472,943.

The following table summarizes information concerning options and warrants outstanding and exercisable:

	Stock Options					Warrants
	Weighted					Weighted
	Average					Average
	Shares			price per share		Shares			price per share		Shares			Total Exercisable
Balance outstanding, December 31, 2020		1,355,901		$	0.69		14,434,702		$	3.50		15,790,603			15,302,830
Granted		24,000			2.17		1,374,600			3.61		1,398,600			-
Exercised		(21,411	)		0.69		-			-		(21,411	)		-
Expired/forfeited		(8,444	)		0.69		(105,495	)	$	3.50		(113,939	)		-
Balance outstanding, June 30, 2021		1,350,046		$	0.72		15,703,807		$	3.50		17,053,853			16,699,039

In the six months ended June 30, 2021 the Company issued 24,000 stock options to an employee ($49,135 fair value, $2.17 exercise price, three-year vesting term and ten-year expiration term). As of June 30, 2021, the 1,350,046 stock options outstanding have a $0.72 weighted average exercise price and 8.22 years weighted average remaining term. Of these options, 995,232 are currently exercisable.

Common Stock and Warrant Issuances

As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates.

	Loan Issuance	Loan		Shares		Defined		Defined
Loan	Date	Principal		Issuable		Date		Frequency
Loan 1	July 21, 2020	$	115,000		5,000		September 30, 2020		Monthly
Loan 2	September 21, 2020	$	345,000		12,500		November 16, 2020		Weekly
Loan 3	September 23, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 4	September 25, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 5	October 22, 2020	$	115,000		12,500		December 1, 2020		Weekly

For our loan dated December 23, 2020, we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the six months ended June 30, 2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five-year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021.

During the six months ended June 30, 2021, we issued 1,642,982 shares of common stock with a fair value of approximately $3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 139,700 shares with a fair value of $349,250 for conversions of debt principal and interest, 21,411 shares for stock option exercises (at an exercise price of $0.69 per share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value of $112,877 for Common Stock issued with debt. During this period, we also issued 1,374,600 warrants (three to five-year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $1.7 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind.

During the six months ended June 30, 2020, we issued to Series AA holders 64,388 shares of common stock for dividends totaling of $176,748 issued in stock in lieu of cash. During this period the Company also issued 593,277 shares of restricted common stock at a fair value of $1,693,251 to accredited investors and consultants. 420,746 of the shares with a fair value of $1,317,649 were issued for conversions of debt principal and interest; 81,031 of the shares with a fair value of $159,784 were issued for debt extensions and interest payments; 66,500 shares with a fair value of $127,855 were issued to settle an accrued liability; and 25,000 shares with a fair value of $87,963 were issued for services rendered.

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Subsequent Events

6 Months Ended

Jun. 30, 2021

Subsequent Events [Abstract]

Subsequent Events

  7) Subsequent Events

From July 1, 2021 through August 10, 2021 the Company received seven new fixed rate convertible loans for a total of $1.5 million. The Company issued 125,000 shares of common stock and 161,000 warrants (3-year and 5-year lives and $3.50 strike price) as fees paid to lenders. These loans have fixed conversion rates of $2.50-$3.00 (one has a variable conversion rate), carry annual interest rates ranging from 10% to 12%, and terms ranging from six to twelve months. One of the loans (for $735,000) is secured by the assets of PBI Agrochem, Inc., a newly incorporated subsidiary of the Company.

In this time period, the Company retired loans with a principal amount of $973,408 (dated June 8, 2018, November 13, 2018, February 21, 2019, June 19, 2019, July 1, 2019, July 19, 2019, and November 1, 2019) for $167,954 and 763,420 shares of common stock (settling principal, interest and fees) and reduced the principal of another loan with a $100,000 cash payment. After this activity, the Company had $741,687 of principal outstanding with two lenders subject to the standstill and forbearance agreements, which expired March 31, 2021 (see Note 5).

The Company is obligated to issue 665,000 shares of common stock to certain lenders for the quarter ended June 30, 2021, but has not issued the shares. The Company and the lenders are negotiating in good faith to resolve these loans and expect to reach a settlement in the coming month.

During this period, the Company also received $900,000 from the sale of Series AA shares (issuing 360,000 warrants with a five-year term and strike price of $3.50), and repaid $103,600 of related party loans.

In this time period, the Company also entered into a Merchant Cash lender agreement (collecting $66,677) and repaid an existing Merchant Cash lender for $62,832. Under this agreement, the Company pays $1,278 each business day to the lender. During this period, the Company also borrowed and repaid a $300,000 line-of-credit from a second lender.

ITEM 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations

CAUTIONARY NOTE REGARDING FORWARD-LOOKING STATEMENTS

This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended (the “Securities Act”) and Section 21E of the Securities Exchange Act of 1934, as amended (the “Exchange Act”). In some cases, forward-looking statements are identified by terms such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “anticipates,” “believes,” “estimates,” “projects,” “predicts,” “potential” and similar expressions intended to identify forward-looking statements. Such statements include, without limitation, statements regarding:

● our need for, and our ability to raise, additional equity or debt financing on acceptable terms, if at all;

● our need to take additional cost reduction measures, cease operations or sell our operating assets, if we are unable to obtain sufficient additional financing;

● our belief that we will have sufficient liquidity to finance normal operations for the foreseeable future;

● the options we may pursue in light of our financial condition;

● the potential applications for Ultra Shear Technology (UST);

● the potential applications of the BaroFold high-pressure protein refolding and disaggregation technology

● the amount of cash necessary to operate our business;

● the anticipated uses of grant revenue and the potential for increased grant revenue in future periods;

● our plans and expectations with respect to our continued operations;

● the expected increase in the number of pressure cycling technology (“PCT”) and constant pressure (“CP”) based units that we believe will be installed and the expected increase in revenues from the sale of consumable products, extended service contracts, and biopharma contract services;

● our belief that PCT has achieved initial market acceptance in the mass spectrometry and other markets;

● the expected development and success of new instrument and consumables product offerings;

● the potential applications for our instrument and consumables product offerings;

● the expected expenses of, and benefits and results from, our research and development efforts;

● the expected benefits and results from our collaboration programs, strategic alliances and joint ventures;

● our expectation of obtaining additional research grants from the government in the future;

● our expectations of the results of our development activities funded by government research grants;

● the potential size of the market for biological sample preparation, biopharma contract services and Ultra Shear Technology;

● general economic conditions;

● the anticipated future financial performance and business operations of our company;

● our reasons for focusing resources in the market for genomic, proteomic, lipidomic and small molecule sample preparation;

● the importance of mass spectrometry as a laboratory tool;

● the advantages of PCT over other current technologies as a method of biological sample preparation and protein characterization in biomarker discovery, forensics, and histology, as well as for other applications;

● the capabilities and benefits of our PCT Sample Preparation System, consumables and other products;

● our belief that laboratory scientists will achieve results comparable with those reported to date by certain research scientists who have published or presented publicly on PCT and our other products and services;

● our ability to retain our core group of scientific, administrative and sales personnel; and

● our ability to expand our customer base in sample preparation and for other applications of PCT, as well as for our other products and services in both the BaroFold and Ultra Shear Technology areas.

These forward-looking statements are only predictions and involve known and unknown risks, uncertainties and other factors that may cause our actual results, levels of activity, performance or achievements to be materially different from any future results, levels of activity, performance or achievements, expressed or implied, by such forward-looking statements. Also, these forward-looking statements represent our estimates and assumptions only as of the date of this Quarterly Report on Form 10-Q. Except as otherwise required by law, we expressly disclaim any obligation or undertaking to release publicly any updates or revisions to any forward-looking statement contained in this Quarterly Report on Form 10-Q to reflect any change in our expectations or any change in events, conditions or circumstances on which any of our forward-looking statements are based. Factors that could cause or contribute to differences in our future financial and other results include those discussed in the risk factors set forth in Part I, Item 1A of our Annual Report on Form 10-K for the year ended December 31, 2020 and in this Report. We qualify all of our forward-looking statements by these cautionary statements.

OVERVIEW:

We are a leader in the development and sale of innovative, broadly enabling, pressure-based platform solutions for the worldwide life sciences industry. Our solutions are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus has historically been in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technology expertise in two new platform technology areas: (1) the use of our patented technology from BaroFold, Inc. for gently controlled disaggregation and refolding of biotherapeutic proteins (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies.

Developments and Accomplishments:

We reported the following accomplishments during the first half of 2021:

● On May 13, 2021, PBI was awarded three additional patents for its revolutionary Ultra Shear Technology™ (UST™) platform, all entitled “System for High Pressure, High Shear Processing of Fluids”. The new patents, awarded in Japan (No. 6843063), Australia (No. 2016243553), and China (ZL201680026865.2), bring the Company’s Intellectual Property (“IP”) estate to a total of 6 issued patents for UST and 29 issued patents overall.

● On April 14, 2021, PBI finalized terms and executed a new letter of intent to purchase the assets of an internationally-based developer and supplier of organically natural, eco-friendly agrochemicals.

● On March 15, 2021, PBI discussed partnerships with Leica Microsystems (cancer diagnostics) and Ohio State University (food industry consortium) with the Stock Day Podcast.

● On March 4, 2021, PBI reported that the transformative impact of the food industry consortium formed by Pressure BioSciences and Ohio State University was discussed in a showcase video from Emerging Technology Insider.

● On February 24, 2021, PBI and Ohio State University announced the formation of a food industry consortium to advance commercialization of the Company’s Ultra Shear Technology (UST) platform.

● On February 8, 2021, PBI announced plans to acquire the assets of a global eco-friendly agrochemical supplier.

● On January 20, 2021, PBI targeted a revolution in effectiveness of therapeutics via improved drug delivery and dosing safety when the Company announced a collaboration with SinuSys Corp to improve and optimize their lead sinus health product candidate prior to Phase IIb trials.

Results of Operations

The following disclosure compares the results of operations for the quarter ended June 30, 2021 (“Q2 2021”) with June 30, 2020 (“Q2 2020”) and compares the six months ended June 30, 2021 with June 30, 2020.

Products and Services Revenue

We recognized total revenue of $608,927 for Q2 2021 compared to $268,154 for Q2 2020, a 127% increase. For the year-to-date periods ending on June 30, 2021 and 2020 we recognized total revenue of $1,168,801 and $522,027, respectively, a 124% increase.

Cost of Products and Services

The cost of products and services was $286,660 for Q2 2021 compared to $134,882 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 our cost of products and services was $512,935 and $310,028, respectively. Gross profit margin on products and services increased from a larger portion of biopharma services that have higher profit margins (to 52.9% in Q2 2021 from 49.6% in Q2 2020 and to 56.1%in the year-to-date period ended June 30, 2021 from 40.6% in the year-to-date period ended June 30, 2020).

Research and Development

Research and development expenses were $256,507 for Q2 2021 compared to $294,602 for Q2 2020. The reported decrease of 13% was attributable to a decrease in outside services. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $556,450 and $560,292, respectively. The reported decrease was 1%.

Selling and Marketing

Selling and marketing expenses were $92,813 for Q2 2021 compared to $162,098 for Q2 2020. The reported decreases were primarily attributable to reduced employees in sales and marketing. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $186,141 and $351,214, respectively.

General and Administrative

General and administrative expenses were $619,286 for Q2 2021 compared to $1,007,215 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $1,634,716 and $2,026,225 respectively. The reported decreases were attributable to lower investor relations expenses.

Operating Loss

Operating loss was $646,339 for Q2 2021 compared to $1,330,643 for Q2 2020, a reduction of $684,304, or 51.4%. For the year to date periods ending of June 30, 2021 and 2020 the operating loss was $1,721,441 and $2,725,732, respectively, a $1,004,291 reduction, or 36.8%. The reported decreases were primarily attributable to revenue increases and decreases in costs and expenses in 2021.

Interest Expense, net

Interest expense was $3,526,141 for Q2 2021 compared to $1,724,879 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these expenses were $8,194,205 and $3,296,679, respectively. The increases were primarily attributable to the increase in convertible and other debt and the issuance of common stock for interest paid-in-kind.

Unrealized (loss) gain on investment in equity securities

Unrealized loss on investments in equity securities was $134,477 for Q2 2021 compared to an unrealized gain of $196,891 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 the unrealized loss was $242,380 and an unrealized gain of $346,262, respectively. The reported change was attributable to movement in the market price of the Company’s investment in Nexity.

Loss on extinguishment of liabilities

In connection with debt extensions and forgiveness, we recognized net losses of $498,226 for Q2 2021 compared to $1,710,151 for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 these losses were $1,223,385 and $2,846,518, respectively. The decreases were principally related to the gain recognized on the forgiveness of our first PPP loan of $367,039.

Net loss attributable to common stockholders

Net loss attributable to common stockholders was $5,149,342 ($0.90 per share) for Q2 2021 compared to $4,965,752 ($1.70 per share) for Q2 2020. For the year-to-date periods ending on June 30, 2021 and 2020 the losses were $12,188,028 ($2.29 per share) and $9,244,223 ($3.34 per share), respectively. The decreases in loss per share were principally attributable to the increase in weighted shares outstanding.

Liquidity and Financial Condition

We have experienced negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of June 30, 2021, we did not have adequate working capital resources to satisfy our current liabilities and as a result, we have substantial doubt regarding our ability to continue as a going concern. As described in Notes 5 and 6 of the accompanying consolidated financial statements, we have been successful in raising debt and equity capital. We received $4.0 million in net proceeds from loans and sales of preferred stock in the six months ended June 30, 2021. We have efforts in place to continue to raise cash through debt and equity offerings. (See Note 7 to the financial statements)

We will need substantial additional capital to fund our operations in future periods. If we are unable to obtain financing on acceptable terms, or at all, we will likely be required to cease our operations, pursue a plan to sell our operating assets, or otherwise modify our business strategy, which could materially harm our future business prospects.

Net cash used in operations for the six months ended June 30, 2021 was $2,090,727 as compared to $2,800,494 for the three months ended June 30, 2020.

Net cash used in investing activities for the six months ended June 30, 2021 was $3,962 compared to $532,913 in the six months ended June 30, 2020.

Net cash provided by financing activities for the six months ended June 30, 2021 was $2,122,774 as compared to $3,345,240 for the six months ended June 30, 2020. The cash flows from financing activities in the six months ended June 30, 2021 included $100,000 from the sale of Series AA preferred stock, $4.0 million loan proceeds from convertible debt and other debt. In this period, cash flow from financing was reduced by debt payments of $1,200,996 on convertible debt, and $744,041 on other debt.

ITEM 3. QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK

This Item 3 is not applicable to us as a smaller reporting company and has been omitted.

ITEM 4. CONTROLS AND PROCEDURES

We maintain disclosure controls and procedures that are designed to ensure that information required to be disclosed in our Securities Exchange Act of 1934 filings are recorded, processed, summarized and reported within the time periods specified in the SEC’s rules and forms, and that such information is accumulated and communicated to our management, including our President and Chief Executive Officer (Principal Executive Officer) and Chief Financial Officer (Principal Financial Officer), as appropriate, to allow timely decisions regarding required disclosure. In designing and evaluating the disclosure controls and procedures, management recognized that controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives, as ours are designed to do, and management was necessarily required to apply its judgment in evaluating the cost-benefit relationship of possible controls and procedures.

As of June 30, 2021, we carried out an evaluation, under the supervision and with the participation of our management, including our Principal Executive Officer and Principal Financial Officer, of the effectiveness of the design and operation of our disclosure controls and procedures, as defined in Rules 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934. Based upon that evaluation, our Principal Executive Officer and Principal Financial Officer concluded that our disclosure controls and procedures were not effective.

Our conclusion that our disclosure controls and procedures were not effective as of June 30, 2021 is due to the continued presence of the material weaknesses in our internal control over financial reporting identified in our Annual Report on Form 10-K for the year ended December 31, 2020. These material weaknesses are the following:

●	We identified a lack of sufficient segregation of duties. Specifically, this material weakness is such that the design over these areas relies primarily on detective controls and could be strengthened by adding preventative controls to properly safeguard Company assets.
●	Management has identified a lack of sufficient personnel in the accounting function due to our limited resources with appropriate skills, training and experience to perform the review processes to ensure the complete and proper application of generally accepted accounting principles, particularly as it relates to valuation of warrants and other complex debt /equity transactions. Specifically, this material weakness resulted in audit adjustments to the annual consolidated financial statements and revisions to related disclosures, valuation of warrants and other equity transactions.
●	Limited policies and procedures that cover recording and reporting of financial transactions.
●	Lack of multiple levels of review over the financial reporting process
We continue to plan to remediate those material weaknesses as follows:
●	Improve the effectiveness of the accounting group by augmenting our existing resources with additional consultants or employees to assist in the analysis and recording of complex accounting transactions, and to simultaneously achieve desired organizational structuring for improved segregation of duties. We plan to mitigate this identified deficiency by hiring an independent consultant once we generate significantly more revenue or raise significant additional working capital.
●	Improve expert review and achieve desired segregation procedures by strengthening cross approval of various functions including quarterly internal audit procedures where appropriate once we generate significantly more revenue or raise significantly more working capital.

During the period covered by this Report, we implemented and performed additional substantive procedures, such as supervisory review of work papers and consistent use of financial models used in equity valuations, to ensure our consolidated financial statements as of and for the three-month period ended June 30, 2021, are fairly stated in all material respects in accordance with GAAP. We have not, however, been able to fully remediate the material weaknesses due to our limited financial resources. Our remediation efforts are largely dependent upon our securing additional financing to cover the costs of implementing the changes required. If we are unsuccessful in securing such funds, remediation efforts may be adversely affected in a material manner.

Except as described above, there have been no changes in our internal controls over financial reporting that occurred during the period ended June 30, 2021 that have materially affected, or are reasonably likely to materially affect, our internal controls over financial reporting.

PART II. OTHER INFORMATION

Item 1. Legal Proceedings

We are not currently involved in any litigation that we believe could have a material adverse effect on our financial condition or results of operations. There is no action, suit, proceeding, inquiry or investigation before or by any court, public board, government agency, self-regulatory organization or body pending or, to the knowledge of the executive officers of our company or any of our subsidiaries, threatened against or affecting our company, our common stock, any of our subsidiaries or of our companies or our subsidiaries’ officers or directors in their capacities as such, in which an adverse decision could have a material adverse effect.

Item 1A. Risk Factors

Factors that could cause or contribute to differences in our future financial and operating results include those discussed in the risk factors set forth in Item 1 of our Annual Report on Form 10-K for the year ended December 31, 2020 and in this Item 1A. The risks described in our Form 10-K and this Report are not the only risks that we face. Additional risks not presently known to us or that we do not currently consider significant may also have an adverse effect on the Company. If any of the risks actually occur, our business, results of operations, cash flows or financial condition could suffer.

There have been no material changes to the risk factors set forth in Item 1A of our 10-K for the year ended December 31, 2020 other than the following:

We owe over $9 million to one lender with such loans secured by a security interest in all of our assets. If we default under our obligations pursuant to such loans, the lender could foreclose on all of our assets which could require us to cease our operations.

Through June 30, 2021, we have issued Notes to the same holder such that the current gross amount owed to the holder is approximately $9.1 million. Our obligations under the Notes and the transaction documents relating to the Notes are secured by a security interest in all of our assets. As a result, if we default under our obligations under the Notes or the transaction documents, the holders of the Notes, acting through their appointed agent, could foreclose on their security interests and liquidate some or all of these assets, which could harm our business, financial condition and results of operations and could require us to reduce or cease operations. In addition, the pledge of these assets and other restrictions may limit our flexibility in raising capital for other purposes. Because all of our assets are pledged under these financing arrangements, our ability to incur additional secured indebtedness or to sell or dispose of assets to raise capital may be impaired, which could have an adverse effect on our financial flexibility.

The holders of our Common Stock could suffer substantial dilution due to our corporate financing practices.

The holders of our common stock could suffer substantial dilution due to our corporate financing practices which, in the past few years has included private placements. As of June 30, 2021, we had 6,260,884 shares outstanding. As of June 30, 2021, if all of the outstanding shares of Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H Convertible Preferred Stock, Series H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock and Series AA Convertible Preferred Stock were converted into shares of common stock and all outstanding options and warrants to purchase shares of common stock were exercised and all fixed rate convertible notes and debentures were converted, each as of June 30, 2021, an additional 30,719,832 shares of common stock would be issued and outstanding. The full cash exercise of the options and warrants would result in approximately $55.9 million in cash proceeds to the Company. This additional issuance of shares of common stock would cause immediate and substantial dilution to our existing stockholders and could cause a significant reduction in the market price of our common stock.

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds

Except where noted, all the securities discussed in this Part II, Item 2 were issued in reliance on the exemption under Section 4(a)(2) of the Securities Act.

During the six months ended June 30, 2021, we issued 1,642,982 shares of common stock with a fair value of approximately $3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 139,700 shares with a fair value of $349,250 for conversions of debt principal and interest, 21,411 shares for stock option exercises (at an exercise price of $0.69 per share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value of $112,877 for Common Stock issued with debt. During this period, we also issued 1,374,600 warrants (three to five-year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $1.7 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind.

During the six months ended June 30, 2020, we issued to Series AA holders 64,388 shares of common stock for dividends totaling of $176,748 issued in stock in lieu of cash. During this period the Company also issued 593,277 shares of restricted common stock at a fair value of $1,693,251 to accredited investors and consultants. 420,746 of the shares with a fair value of $1,317,649 were issued for conversions of debt principal and interest; 81,031 of the shares with a fair value of $159,784 were issued for debt extensions and interest payments; 66,500 shares with a fair value of $127,855 were issued to settle an accrued liability; and 25,000 shares with a fair value of $87,963 were issued for services rendered.

Item 3. Defaults upon Senior Securities

None.

Item 4. Mine Safety Disclosures

Not applicable.

Item 5. Other Information

None.

Item 6. Exhibits

Exhibits
31.1*	Certification by the Principal Executive Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))
31.2*	Certification by the Principal Financial Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))
32.1**	Certification by the Principal Executive Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*
32.2**	Certification by the Principal Financial Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*
101.INS*	XBRL Instance Document
101.SCH*	XBRL Taxonomy Extension Schema Document
101.CAL*	XBRL Taxonomy Extension Calculation Linkbase Document
101.DEF*	XBRL Taxonomy Extension Definition Linkbase Document
101.LAB*	XBRL Taxonomy Extension Label Linkbase Document
101.PRE*	XBRL Taxonomy Extension Presentation Linkbase Document

* Filed herewith.

** In accordance with SEC Release 33-8238, Exhibits 32.1 and 32.2 are furnished and not filed.

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant caused this report to be signed on its behalf by the undersigned thereunto duly authorized.

	PRESSURE BIOSCIENCES, INC.
Date: August 18, 2021	By:	/s/ Richard T. Schumacher
		Richard T. Schumacher
		President & Chief Executive Officer
		(Principal Executive Officer and Principal Financial Officer)

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Summary of Significant Accounting Policies (Policies)

6 Months Ended

Jun. 30, 2021

Accounting Policies [Abstract]

Basis of Presentation

Basis of Presentation

The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2020.

Use of Estimates

Use of Estimates

The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements.

Principles of Consolidation

Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiary PBI BioSeq, Inc. All intercompany accounts and transactions have been eliminated in consolidation.

Revenue Recognition

Revenue Recognition

We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, and ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers. Revenue is measured based on a consideration specified in a contract with a customer, and excludes any sales incentives and amounts collected on behalf of third parties. We enter into sales contracts that may consist of multiple distinct performance obligations where certain performance obligations of the sales contract are not delivered in one reporting period. We measure and allocate revenue according to ASC 606-10.

We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation.

Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue.

Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods.

Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue.

The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components.

Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements.

We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

a)	The fair value of the asset or service involved is not determinable.
b)	The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange.
c)	The transaction lacks commercial substance.

We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold.

We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases.

Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract.

Disaggregation of revenue

In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition.

In thousands of US dollars ($)	Three Months Ended June 30,				Six Months Ended June 30,
Primary geographical markets	2021		2020		2021		2020
North America	$	477	$	162	$	685	$	307
Europe		103		3		187		4
Asia		29		103		297		211
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Major products/services lines		2021		2020		2021		2020
Hardware	$	336	$	122	$	713	$	217
Consumables		44		51		146		107
Contract research services		136		34		142		44
Sample preparation accessories		40		33		69		58
Technical support/extended service contracts		34		17		58		36
Shipping and handling		16		6		35		15
Other		3		5		6		45
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Timing of revenue recognition		2021		2020		2021		2020
Products transferred at a point in time	$	440	$	217	$	969	$	442
Services transferred over time		169		51		200		80
	$	609	$	268	$	1,169	$	522

Contract balances

In thousands of US dollars ($)	June 30, 2021		December 31, 2020
Receivables, which are included in ‘Accounts Receivable’	$	630	$	131
Contract liabilities (deferred revenue)		57		67

Transaction price allocated to the remaining performance obligations.

The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period.

 Schedule of Future Related to Performance Obligations

In thousands of US dollars ($)	2021		2022		Total
Extended warranty service	$	49		8	$	57

All consideration from contracts with customers is included in the amounts presented above.

Contract Costs

The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing.

Concentrations

Concentrations

Credit Risk

Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories.

The following table illustrates the level of concentration as a percentage of total revenues during the three and six months ended June 30, 2021 and 2020.

	For the Three Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		71	%
Federal Agencies		14	%		2	%

	For the Six Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		70	%
Federal Agencies		8	%		4	%

The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of June 30, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable.

	June 30, 2021			December 31, 2020
Top Five Customers		56	%		89	%
Federal Agencies		11	%		10	%

Product Supply

Product Supply

We utilize a contract assembler for our Barocycler® 2320EXT. They provide us with precision manufacturing services that include management support services to meet our specific application and operational requirements. Among the services provided to us are:

● CNC Machining

● Contract Assembly & Kitting

● Component and Subassembly Design

● Inventory Management

● ISO certification

At this time, we believe that outsourcing contract assembly of our Barocycler® 2320EXT is the most cost-effective method for us to obtain ISO Certified, CE and CSA Marked instruments. The Company also continues to evaluate the potential economic benefits of bringing instrument assembly in-house.

We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments.

Investment in Equity Securities

Investment in Equity Securities

As of June 30, 2021, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company).

We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income.

As of June 30, 2021, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $274,621. We recorded $242,380 as an unrealized loss during the six months ended June 30, 2021 for changes in market value.

Computation of Loss per Share

Computation of Loss per Share

Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss.

The following table illustrates our computation of loss per share for the three and six months ended June 30, 2021 and 2020:

	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2021			2020			2021			2020
Numerator:
Net loss attributable to common stockholders	$	(5,149,342	)	$	(4,965,752	)	$	(12,188,028	)	$	(9,244,223	)
Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding		5,748,711			2,914,659			5,312,172			2,765,132
Loss per common share – basic and diluted	$	(0.90	)	$	(1.70	)	$	(2.29	)	$	(3.34	)

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms.

	As of June 30,
	2021		2020
Stock options		1,350,046		1,392,370
Convertible debt		5,083,187		3,905,867
Common stock warrants		15,703,807		12,761,126
Convertible preferred stock:
Series D Convertible Preferred Stock		25,000		25,000
Series G Convertible Preferred Stock		26,857		26,857
Series H Convertible Preferred Stock		33,334		33,334
Series H2 Convertible Preferred Stock		70,000		70,000
Series J Convertible Preferred Stock		115,267		115,267
Series K Convertible Preferred Stock		229,334		229,334
Series AA Convertible Preferred Stock		8,083,000		7,939,000
		30,719,832		26,498,155

Accounting for Stock-Based Compensation Expense

Accounting for Stock-Based Compensation Expense

We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant.

Determining Fair Value of Stock Option Grants

Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period.

Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted.

Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award.

Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term.

Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense.

The Company recognized stock-based compensation expense of $63,458 and $65,341 for the three months ended June 30, 2021 and 2020, respectively. The Company recognized stock-based compensation expense of $124,695 and $307,110 for the six months ended June 30, 2021 and 2020, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations:

	For the Three Months Ended				For the Six Months Ended
	June 30,				June 30,
	2021		2020		2021		2020
Cost of sales	$	5,107	$	5,107	$	10,160	$	13,063
Research and development		26,491		26,137		52,353		64,963
Selling and marketing		5,887		6,358		10,482		20,294
General and administrative		25,973		27,739		51,700		208,790
Total stock-based compensation expense	$	63,458	$	65,341	$	124,695	$	307,110

Fair Value of Financial Instruments

Fair Value of Financial Instruments

Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. Long-term liabilities include debt and deferred revenue with a carrying value that approximates fair value.

Fair Value Measurements

Fair Value Measurements

The Company follows the guidance of FASB ASC Topic 820, “Fair Value Measurements and Disclosures” (“ASC 820”) as it related to all financial assets and financial liabilities that are recognized or disclosed at fair value in the financial statements on a recurring basis.

The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement.

Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management.

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of June 30, 2021:

			Fair value measurements at June 30, 2021 using:
	June 30, 2021		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities	$	274,621	$	274,621		-		-
Total Financial Assets	$	274,621	$	274,621	$	-	$	-

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020:

			Fair value measurements at December 31, 2020 using:
	December 31, 2020		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities		517,001		517,001		-		-
Total Financial Assets	$	517,001	$	517,001	$	-	$	-

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Summary of Significant Accounting Policies (Tables)

6 Months Ended

Jun. 30, 2021

Accounting Policies [Abstract]

Schedule of Disaggregation of Revenue

In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition.

In thousands of US dollars ($)	Three Months Ended June 30,				Six Months Ended June 30,
Primary geographical markets	2021		2020		2021		2020
North America	$	477	$	162	$	685	$	307
Europe		103		3		187		4
Asia		29		103		297		211
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Major products/services lines		2021		2020		2021		2020
Hardware	$	336	$	122	$	713	$	217
Consumables		44		51		146		107
Contract research services		136		34		142		44
Sample preparation accessories		40		33		69		58
Technical support/extended service contracts		34		17		58		36
Shipping and handling		16		6		35		15
Other		3		5		6		45
	$	609	$	268	$	1,169	$	522

	Three Months Ended June 30,				Six Months Ended June 30,
Timing of revenue recognition		2021		2020		2021		2020
Products transferred at a point in time	$	440	$	217	$	969	$	442
Services transferred over time		169		51		200		80
	$	609	$	268	$	1,169	$	522

Schedule of Contract Balances

Contract balances

In thousands of US dollars ($)	June 30, 2021		December 31, 2020
Receivables, which are included in ‘Accounts Receivable’	$	630	$	131
Contract liabilities (deferred revenue)		57		67

Schedule of Future Related to Performance Obligations

The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period.

 Schedule of Future Related to Performance Obligations

In thousands of US dollars ($)	2021		2022		Total
Extended warranty service	$	49		8	$	57

Schedule of Customer Concentration Risk Percentage

The following table illustrates the level of concentration as a percentage of total revenues during the three and six months ended June 30, 2021 and 2020.

	For the Three Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		71	%
Federal Agencies		14	%		2	%

	For the Six Months Ended
	June 30,
	2021			2020
Top Five Customers		50	%		70	%
Federal Agencies		8	%		4	%

The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of June 30, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable.

	June 30, 2021			December 31, 2020
Top Five Customers		56	%		89	%
Federal Agencies		11	%		10	%

Schedule of Computation of Loss Per Share

The following table illustrates our computation of loss per share for the three and six months ended June 30, 2021 and 2020:

	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2021			2020			2021			2020
Numerator:
Net loss attributable to common stockholders	$	(5,149,342	)	$	(4,965,752	)	$	(12,188,028	)	$	(9,244,223	)
Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding		5,748,711			2,914,659			5,312,172			2,765,132
Loss per common share – basic and diluted	$	(0.90	)	$	(1.70	)	$	(2.29	)	$	(3.34	)

Schedule of Anti-dilutive Securities Excluded from Computation of Earnings Per Share

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms.

	As of June 30,
	2021		2020
Stock options		1,350,046		1,392,370
Convertible debt		5,083,187		3,905,867
Common stock warrants		15,703,807		12,761,126
Convertible preferred stock:
Series D Convertible Preferred Stock		25,000		25,000
Series G Convertible Preferred Stock		26,857		26,857
Series H Convertible Preferred Stock		33,334		33,334
Series H2 Convertible Preferred Stock		70,000		70,000
Series J Convertible Preferred Stock		115,267		115,267
Series K Convertible Preferred Stock		229,334		229,334
Series AA Convertible Preferred Stock		8,083,000		7,939,000
		30,719,832		26,498,155

Schedule of Stock Based Compensation Expense

	For the Three Months Ended				For the Six Months Ended
	June 30,				June 30,
	2021		2020		2021		2020
Cost of sales	$	5,107	$	5,107	$	10,160	$	13,063
Research and development		26,491		26,137		52,353		64,963
Selling and marketing		5,887		6,358		10,482		20,294
General and administrative		25,973		27,739		51,700		208,790
Total stock-based compensation expense	$	63,458	$	65,341	$	124,695	$	307,110

Schedule of Assets and Liabilities Measured at Fair Value on Recurring Basis

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of June 30, 2021:

			Fair value measurements at June 30, 2021 using:
	June 30, 2021		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities	$	274,621	$	274,621		-		-
Total Financial Assets	$	274,621	$	274,621	$	-	$	-

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020:

			Fair value measurements at December 31, 2020 using:
	December 31, 2020		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities		517,001		517,001		-		-
Total Financial Assets	$	517,001	$	517,001	$	-	$	-

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Commitments and Contingencies (Tables)

6 Months Ended

Jun. 30, 2021

Commitments and Contingencies Disclosure [Abstract]

Schedule of Future Minimum Rental Payments Required Under Operating Leases

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms as of June 30, 2021:

2021	$	85,391
2022		87,383
2023		87,383
Total minimum payments required	$	260,157

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Convertible Debt and Other Debt (Tables)

6 Months Ended

Jun. 30, 2021

Debt Disclosure [Abstract]

Schedule of Convertible Debts and Outstanding Balances

The specific terms of the convertible notes and outstanding balances as of June 30, 2021 are listed in the tables below.

Inception Date		Term		Loan Amount		Outstanding balance with OID		Original Issue Discount (OID)		Interest Rate			Conversion Price		Deferred Finance Fees		Discount for conversion feature and warrants/shares
May 17, 2018 (1) (2)			12 months	$	380,000	$	166,703	$	15,200		8	%	$	2.50	$	15,200	$	332,407
June 8, 2018 (1) (4)	(1)		6 months	$	50,000	$	50,000	$	2,500		2	%	$	7.50	$	2,500	$	3,271
October 19, 2018 (1)			6 months	$	100,000	$	100,000	$	-		5	%	$	7.50	$	-	$	-
November 13, 2018 (1) (3) (4)			6 months	$	200,000	$	78,508	$	-		5	%	$	2.50	$	-	$	168,634
January 3, 2019 (1) (4)			6 months	$	50,000	$	50,000	$	2,500		24	%	$	7.50	$	2,500	$	-
February 21, 2019 (1) (2)			12 months	$	215,000	$	215,000	$	-		4	%	$	2.50	$	15,000	$	107,709
March 18, 2019 (1)			6 months	$	100,000	$	100,000	$	-		4	%	$	7.50	$	-	$	10,762
June 4, 2019 (1) (2)			9 months	$	500,000	$	302,484	$	-		8	%	$	2.50	$	40,500	$	70,631
June 19, 2019 (1) (2)			12 months	$	105,000	$	105,000	$	-		4	%	$	2.50	$	5,000	$	2,646
June 7, 2019 (1) (4)			6 months	$	125,000	$	6,746	$	-		5	%	$	7.50	$	-	$	18,254
July 1, 2019 (1) (2)			12 months	$	107,500	$	107,500	$	-		4	%	$	2.50	$	7,500	$	85,791
July 19, 2019 (1) (2)			12 months	$	115,000	$	115,000	$	-		4	%	$	2.50	$	5,750	$	15,460
July 19, 2019 (1) (2)			12 months	$	130,000	$	130,000	$	-		6	%	$	2.50	$	6,500	$	-
September 27, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	13,759
October 24, 2019 (1) (2)			12 months	$	78,750	$	78,750	$	-		4	%	$	2.50	$	3,750	$	-
November 1, 2019 (1) (2)			12 months	$	270,000	$	270,000	$	-		6	%	$	2.50	$	13,500	$	-
November 15, 2019 (1)			12 months	$	385,000	$	320,000	$	35,000		10	%	$	2.50	$	35,000	$	90,917
January 2, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	91,606
January 24, 2020 (1)			12 months	$	247,500	$	247,500	$	22,500		10	%	$	2.50	$	22,500	$	89,707
January 29, 2020 (1)			12 months	$	363,000	$	363,000	$	33,000		10	%	$	2.50	$	33,000	$	297,000
February 12, 2020 (1)			12 months	$	275,000	$	275,000	$	25,000		10	%	$	2.50	$	25,000	$	225,000
February 19, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	135,000
March 11, 2020 (1)			12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50	$	30,000	$	232,810
March 13, 2020 (1)			12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50	$	15,000	$	60,705
March 26, 2020 (1)			12 months	$	111,100	$	111,100	$	10,100		10	%	$	2.50	$	10,100	$	90,900
April 8, 2020 (1)			12 months	$	276,100	$	276,100	$	25,100		10	%	$	2.50	$	25,000	$	221,654
April 17, 2020 (1)			12 months	$	143,750	$	143,750	$	18,750		10	%	$	2.50	$	-	$	96,208
April 30, 2020 (1)			12 months	$	546,250	$	546,250	$	71,250		10	%	$	2.50	$	47,500	$	427,500
May 6, 2020 (1)			12 months	$	460,000	$	460,000	$	60,000		10	%	$	2.50	$	40,000	$	360,000
May 18, 2020 (1)			12 months	$	546,250	$	221,250	$	46,250		10	%	$	2.50	$	35,500	$	439,500
June 2, 2020 (1)			12 months	$	902,750	$	652,750	$	92,750		10	%	$	2.50	$	58,900	$	708,500
June 12, 2020 (1)			12 months	$	57,500	$	57,500	$	7,500		10	%	$	2.50	$	5,000	$	45,000
June 22, 2020 (1)			12 months	$	138,000	$	138,000	$	18,000		10	%	$	2.50	$	12,000	$	108,000
July 7, 2020 (1)			12 months	$	586,500	$	586,500	$	76,500		10	%	$	2.50	$	51,000	$	400,234
July 17, 2020 (1)			12 months	$	362,250	$	362,250	$	47,250		10	%	$	2.50	$	31,500	$	185,698
July 29, 2020 (1)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	241,245
July 21, 2020 (1) (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	24,875
August 14, 2020 (1)			12 months	$	762,450	$	462,450	$	69,450		10	%	$	2.50	$	66,300	$	580,124
September 10, 2020			12 months	$	391,000	$	391,000	$	51,000		10	%	$	2.50	$	34,000	$	231,043
September 21, 2020 (5)			12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50	$	30,000	$	66,375
September 23, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	20,500
September 25, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	-	$	19,125
December 3, 2020			12 months	$	299,000	$	299,000	$	39,000		10	%	$	2.50	$	26,000	$	197,882
October 22, 2020 (5)			12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50	$	10,000	$	18,875
December 23, 2020 (1) (5)			6 months	$	1,000,000	$	150,000	$	100,000		10	%	$	2.50	$	-	$	833,536
January 5, 2021 (1)			6 months	$	575,000	$	575,000	$	75,000		18	%	$	2.50	$	-	$	-
February 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	180,000
March 23, 2021			12 months	$	55,000	$	55,000	$	5,000		10	%	$	2.50	$	-	$	36,431
May 24, 2021			6 months	$	54,625	$	54,625	$	7,125		12	%	$	2.50	$	-	$	-
May 6, 2021			12 months	$	402,500	$	402,500	$	52,500		10	%	$	2.50	$	35,000	$	312,551
June 17, 2021			12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50	$	20,000	$	144,760
June 25, 2021			12 months	$	977,500	$	977,500	$	127,500		10	%	$	2.50	$	-	$	773,802
May 20, 2021			12 months	$	180,000	$	180,000	$	30,000		10	%	$	2.50	$	15,000	$	25,824
June 3, 2021			12 months	$	50,000	$	50,000	$	1,500		12	%	$	2.50	$	-	$	7,948
June 28, 2021			12 months	$	350,000	$	350,000	$	35,000		12	%		(6)	$	22,750	$	267,250
						$	13,262,466	$	1,502,225						$	972,500	$	9,117,409

(1)	The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.
(2)	The Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below).
(3)	Interest was capitalized and added to outstanding principal.
(4)	During the year ended December 31, 2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.
(5)	The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.
(6)	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.

Summary of Changes in Convertible Debt and Revolving Note Payable, Net of Unamortized Discounts

	2021
Balance at January 1,	$	7,545,670
Issuance of convertible debt, face value		3,104,625
Deferred financing cost		(506,375	)
Beneficial conversion feature on convertible note		(566,847	)
Debt discount from shares and warrants issued with debt		(1,181,719	)
Payments		(1,200,996	)
Conversion of debt into equity		(150,000	)
Accretion of interest and amortization of debt discount to interest expense		3,864,956
Balance at June 30,		10,909,314
Less: current portion		10,909,314
Convertible debt, long-term portion	$	–

Schedule of Merchant Agreements

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
February 4, 2021		125,000		165,000		55,281		1,032		-
March 11, 2021		125,000		167,500		40,559		1,396		2,500
March 26, 2021		240,000		330,960		129,952		2,364		-
May 3, 2021		200,000		275,800		141,458		1,970		5,000
June 2, 2021		135,000		186,165		115,677		1,400		1,350
	$	825,000	$	1,125,425	$	482,927	$	8,162	$	8,850

The following table shows our Merchant Agreements as of December 31, 2020:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Daily Payment Rate		Deferred Finance Fees
November 5, 2020	$	200,000	$	275,800	$	163,955		1,724	$	-
November 19, 2020		100,000		137,900		85,013		985		-
	$	300,000	$	413,700	$	248,968	$	2,709	$	-

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Stockholders’ Deficit (Tables)

6 Months Ended

Jun. 30, 2021

Equity [Abstract]

Schedule of Concerning Options and Warrants Outstanding and Exercisable

The following table summarizes information concerning options and warrants outstanding and exercisable:

	Stock Options					Warrants
	Weighted					Weighted
	Average					Average
	Shares			price per share		Shares			price per share		Shares			Total Exercisable
Balance outstanding, December 31, 2020		1,355,901		$	0.69		14,434,702		$	3.50		15,790,603			15,302,830
Granted		24,000			2.17		1,374,600			3.61		1,398,600			-
Exercised		(21,411	)		0.69		-			-		(21,411	)		-
Expired/forfeited		(8,444	)		0.69		(105,495	)	$	3.50		(113,939	)		-
Balance outstanding, June 30, 2021		1,350,046		$	0.72		15,703,807		$	3.50		17,053,853			16,699,039

Schedule of Loans Obligated to Issue Shares

As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates.

	Loan Issuance	Loan		Shares		Defined		Defined
Loan	Date	Principal		Issuable		Date		Frequency
Loan 1	July 21, 2020	$	115,000		5,000		September 30, 2020		Monthly
Loan 2	September 21, 2020	$	345,000		12,500		November 16, 2020		Weekly
Loan 3	September 23, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 4	September 25, 2020	$	115,000		12,500		December 1, 2020		Weekly
Loan 5	October 22, 2020	$	115,000		12,500		December 1, 2020		Weekly

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Schedule of Disaggregation of Revenue (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020
Product Information [Line Items]
Revenue	$ 608,927	$ 268,154	$ 1,168,801	$ 522,027
Hardware [Member]
Product Information [Line Items]
Revenue	336,000	122,000	713,000	217,000
Consumables [Member]
Product Information [Line Items]
Revenue	44,000	51,000	146,000	107,000
Contract Research Services [Member]
Product Information [Line Items]
Revenue	136,000	34,000	142,000	44,000
Sample Preparation Accessories [Member]
Product Information [Line Items]
Revenue	40,000	33,000	69,000	58,000
Technical Support/Extended Service Contracts [Member]
Product Information [Line Items]
Revenue	34,000	17,000	58,000	36,000
Shipping and Handling [Member]
Product Information [Line Items]
Revenue	16,000	6,000	35,000	15,000
Others [Member]
Product Information [Line Items]
Revenue	3,000	5,000	6,000	45,000
Transferred at a Point in Time [Member]
Product Information [Line Items]
Revenue	440,000	217,000	969,000	442,000
Transferred Over Time [Member]
Product Information [Line Items]
Revenue	169,000	51,000	200,000	80,000
North America [Member]
Product Information [Line Items]
Revenue	477,000	162,000	685,000	307,000
Europe [Member]
Product Information [Line Items]
Revenue	103,000	3,000	187,000	4,000
Asia [Member]
Product Information [Line Items]
Revenue	$ 29,000	$ 103,000	$ 297,000	$ 211,000

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Schedule of Contract Balances (Details) - USD ($) $ in Thousands	Jun. 30, 2021	Dec. 31, 2020
Accounting Policies [Abstract]
Receivables, which are included in 'Accounts Receivable'	$ 630	$ 131
Contract liabilities (deferred revenue)	$ 57	$ 67

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Schedule of Future Related to Performance Obligations (Details) $ in Thousands	Jun. 30, 2021 USD ($)
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Extended warranty service	$ 57
2021 [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Extended warranty service	49
2022 [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Extended warranty service	$ 8

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Schedule of Customer Concentration Risk Percentage (Details) - Customer Concentration Risk [Member]	3 Months Ended		6 Months Ended		12 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020	Dec. 31, 2020
Revenue Benchmark [Member] | Top Five Customers [Member]
Product Information [Line Items]
Concentration credit risk percentage	50.00%	71.00%	50.00%	70.00%
Revenue Benchmark [Member] | Federal Agencies [Member]
Product Information [Line Items]
Concentration credit risk percentage	14.00%	2.00%	8.00%	4.00%
Accounts Receivable [Member] | Top Five Customers [Member]
Product Information [Line Items]
Concentration credit risk percentage			56.00%		89.00%
Accounts Receivable [Member] | Federal Agencies [Member]
Product Information [Line Items]
Concentration credit risk percentage			11.00%		10.00%

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Schedule of Computation of Loss Per Share (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020
Accounting Policies [Abstract]
Net loss attributable to common stockholders	$ (5,149,342)	$ (4,965,752)	$ (12,188,028)	$ (9,244,223)
Weighted average common stock shares outstanding	5,748,711	2,914,659	5,312,172	2,765,132
Loss per common share – basic and diluted	$ (0.90)	$ (1.70)	$ (2.29)	$ (3.34)

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Schedule of Anti-dilutive Securities Excluded from Computation of Earnings Per Share (Details) - shares	6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	30,719,832	26,498,155
Stock Options [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	1,350,046	1,392,370
Convertible Debt Securities [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	5,083,187	3,905,867
Common Stock Warrants [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	15,703,807	12,761,126
Series D Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	25,000	25,000
Series G Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	26,857	26,857
Series H Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	33,334	33,334
Series H Two Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	70,000	70,000
Series J Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	115,267	115,267
Series K Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	229,334	229,334
Series AA Convertible Preferred Stock [Member]
Antidilutive Securities Excluded from Computation of Earnings Per Share [Line Items]
Total potentially dilutive shares	8,083,000	7,939,000

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Schedule of Stock Based Compensation Expense (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020
Total stock-based compensation expense	$ 63,458	$ 65,341	$ 124,695	$ 307,110
Cost of Sales [Member]
Total stock-based compensation expense	5,107	5,107	10,160	13,063
Research and Development Expense [Member]
Total stock-based compensation expense	26,491	26,137	52,353	64,963
Selling and Marketing Expense [Member]
Total stock-based compensation expense	5,887	6,358	10,482	20,294
General and Administrative Expense [Member]
Total stock-based compensation expense	$ 25,973	$ 27,739	$ 51,700	$ 208,790

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Schedule of Assets and Liabilities Measured at Fair Value on Recurring Basis (Details) - USD ($)	Jun. 30, 2021	Dec. 31, 2020
Financing Receivable, Impaired [Line Items]
Total Financial Assets	$ 274,621	$ 517,001
Fair Value, Inputs, Level 1 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets	274,621	517,001
Fair Value, Inputs, Level 2 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets
Fair Value, Inputs, Level 3 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets
Equity Securities [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets	274,621	517,001
Equity Securities [Member] | Fair Value, Inputs, Level 1 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets	274,621	517,001
Equity Securities [Member] | Fair Value, Inputs, Level 2 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets
Equity Securities [Member] | Fair Value, Inputs, Level 3 [Member]
Financing Receivable, Impaired [Line Items]
Total Financial Assets

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Summary of Significant Accounting Policies (Details Narrative) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020
Entity Listings [Line Items]
Share-based Payment Arrangement, Noncash Expense	$ 63,458	$ 65,341	$ 124,695	$ 307,110
Nexity Global SA [Member]
Entity Listings [Line Items]
Sale of Stock, Number of Shares Issued in Transaction			100,250
Investment Owned, at Fair Value	$ 274,621		$ 274,621
Marketable Securities, Realized Gain (Loss)			$ 242,380

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Schedule of Future Minimum Rental Payments Required Under Operating Leases (Details)	Jun. 30, 2021 USD ($)
Commitments and Contingencies Disclosure [Abstract]
2021	$ 85,391
2022	87,383
2023	87,383
Total minimum payments required	$ 260,157

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Commitments and Contingencies (Details Narrative) - USD ($)	6 Months Ended	12 Months Ended
	Jun. 30, 2021	Dec. 31, 2020
SEC Schedule, 12-29, Real Estate Companies, Investment in Mortgage Loans on Real Estate [Line Items]
Payments for Rent	$ 7,282
Lessee, Operating Lease, Description	The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.
Operating Lease, Liability	$ 221,432
Operating Lease, Weighted Average Discount Rate, Percent	12.00%
Target Discovery Inc [Member]
SEC Schedule, 12-29, Real Estate Companies, Investment in Mortgage Loans on Real Estate [Line Items]
Payment for fees	$ 34,400
Corporate Office [Member]
SEC Schedule, 12-29, Real Estate Companies, Investment in Mortgage Loans on Real Estate [Line Items]
Payments for Rent		$ 6,950
Lease Expiration Date		Dec. 31, 2021

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Schedule of Convertible Debts and Outstanding Balances (Details) - USD ($)		6 Months Ended
		Jun. 30, 2021	Dec. 31, 2020
Debt Instrument [Line Items]
Loan Amount		$ 3,104,625
Outstanding balance with OID		10,909,314	$ 7,545,670
Original Issue Discount (OID)		150,000
Deferred Finance Fees		8,850
Discount for conversion feature and warrants/shares		566,847
Fixed Rate Convertible Notes [Member]
Debt Instrument [Line Items]
Outstanding balance with OID		13,262,466
Original Issue Discount (OID)		1,502,225
Deferred Finance Fees		972,500
Discount for conversion feature and warrants/shares		$ 9,117,409
Fixed Rate Convertible Notes [Member] | Convertible Debt One [Member]
Debt Instrument [Line Items]
Inception Date	[1]	May 17, 2018
Debenture conversion term		12 months
Loan Amount		$ 380,000
Outstanding balance with OID		166,703
Original Issue Discount (OID)		$ 15,200
Interest Rate		8.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,200
Discount for conversion feature and warrants/shares		$ 332,407
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Two [Member]
Debt Instrument [Line Items]
Inception Date	[1],[2]	June 8, 2018
Debenture conversion term		6 months
Loan Amount		$ 50,000
Outstanding balance with OID		50,000
Original Issue Discount (OID)		$ 2,500
Interest Rate		2.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ 2,500
Discount for conversion feature and warrants/shares		$ 3,271
Debt Instrument, Convertible, Conversion Price		$ (7.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Three [Member]
Debt Instrument [Line Items]
Inception Date	[1]	October 19, 2018
Debenture conversion term		6 months
Loan Amount		$ 100,000
Outstanding balance with OID		100,000
Original Issue Discount (OID)		$ (0)
Interest Rate		5.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ (0)
Discount for conversion feature and warrants/shares		$ (0)
Debt Instrument, Convertible, Conversion Price		$ (7.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Four [Member]
Debt Instrument [Line Items]
Inception Date	[1],[2],[3]	November 13, 2018
Debenture conversion term		6 months
Loan Amount		$ 200,000
Outstanding balance with OID		78,508
Original Issue Discount (OID)
Interest Rate		5.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ (0)
Discount for conversion feature and warrants/shares		$ 168,634
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Five [Member]
Debt Instrument [Line Items]
Inception Date	[1],[2]	January 3, 2019
Debenture conversion term		6 months
Loan Amount		$ 50,000
Outstanding balance with OID		50,000
Original Issue Discount (OID)		$ 2,500
Interest Rate		24.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ 2,500
Discount for conversion feature and warrants/shares		$ (0)
Debt Instrument, Convertible, Conversion Price		$ (7.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Six [Member]
Debt Instrument [Line Items]
Inception Date	[1]	February 21, 2019
Debenture conversion term		12 months
Loan Amount		$ 215,000
Outstanding balance with OID		215,000
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 107,709
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Seven [Member]
Debt Instrument [Line Items]
Inception Date	[1]	March 18, 2019
Debenture conversion term		6 months
Loan Amount		$ 100,000
Outstanding balance with OID		100,000
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ (0)
Discount for conversion feature and warrants/shares		$ 10,762
Debt Instrument, Convertible, Conversion Price		$ (7.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Eight [Member]
Debt Instrument [Line Items]
Inception Date	[1]	June 4, 2019
Debenture conversion term		9 months
Loan Amount		$ 500,000
Outstanding balance with OID		302,484
Original Issue Discount (OID)		$ (0)
Interest Rate		8.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 40,500
Discount for conversion feature and warrants/shares		$ 70,631
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Nine [Member]
Debt Instrument [Line Items]
Inception Date	[1]	June 19, 2019
Debenture conversion term		12 months
Loan Amount		$ 105,000
Outstanding balance with OID		105,000
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,000
Discount for conversion feature and warrants/shares		$ 2,646
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Ten [Member]
Debt Instrument [Line Items]
Inception Date	[1],[2]	June 7, 2019
Debenture conversion term		6 months
Loan Amount		$ 125,000
Outstanding balance with OID		6,746
Original Issue Discount (OID)		$ (0)
Interest Rate		5.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ (0)
Discount for conversion feature and warrants/shares		$ 18,254
Debt Instrument, Convertible, Conversion Price		$ (7.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Eleven [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 1, 2019
Debenture conversion term		12 months
Loan Amount		$ 107,500
Outstanding balance with OID		107,500
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 7,500
Discount for conversion feature and warrants/shares		$ 85,791
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twelve [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 19, 2019
Debenture conversion term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,750
Discount for conversion feature and warrants/shares		$ 15,460
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 19, 2019
Debenture conversion term		12 months
Loan Amount		$ 130,000
Outstanding balance with OID		130,000
Original Issue Discount (OID)		$ (0)
Interest Rate		6.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 6,500
Discount for conversion feature and warrants/shares		$ (0)
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	September 27, 2019
Debenture conversion term		12 months
Loan Amount		$ 78,750
Outstanding balance with OID		78,750
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 3,750
Discount for conversion feature and warrants/shares		$ 13,759
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	October 24, 2019
Debenture conversion term		12 months
Loan Amount		$ 78,750
Outstanding balance with OID		78,750
Original Issue Discount (OID)		$ (0)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 3,750
Discount for conversion feature and warrants/shares		$ (0)
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Sixteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	November 1, 2019
Debenture conversion term		12 months
Loan Amount		$ 270,000
Outstanding balance with OID		270,000
Original Issue Discount (OID)		$ (0)
Interest Rate		6.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 13,500
Discount for conversion feature and warrants/shares		$ (0)
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Seventeen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	November 15, 2019 (1)
Debenture conversion term		12 months
Loan Amount		$ 385,000
Outstanding balance with OID		320,000
Original Issue Discount (OID)		$ 35,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 35,000
Discount for conversion feature and warrants/shares		$ 90,917
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Eightteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	January 2, 2020
Debenture conversion term		12 months
Loan Amount		$ 330,000
Outstanding balance with OID		330,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 91,606
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Nineteen [Member]
Debt Instrument [Line Items]
Inception Date	[1]	January 24, 2020
Debenture conversion term		12 months
Loan Amount		$ 247,500
Outstanding balance with OID		247,500
Original Issue Discount (OID)		$ 22,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 22,500
Discount for conversion feature and warrants/shares		$ 89,707
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty [Member]
Debt Instrument [Line Items]
Inception Date	[1]	January 29, 2020 (1)
Debenture conversion term		12 months
Loan Amount		$ 363,000
Outstanding balance with OID		363,000
Original Issue Discount (OID)		$ 33,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 33,000
Discount for conversion feature and warrants/shares		$ 297,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty One [Member]
Debt Instrument [Line Items]
Inception Date	[1]	February 12, 2020 (1)
Debenture conversion term		12 months
Loan Amount		$ 275,000
Outstanding balance with OID		275,000
Original Issue Discount (OID)		$ 25,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 25,000
Discount for conversion feature and warrants/shares		$ 225,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Two [Member]
Debt Instrument [Line Items]
Inception Date	[1]	February 19, 2020 (1)
Debenture conversion term		12 months
Loan Amount		$ 165,000
Outstanding balance with OID		165,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 135,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Three [Member]
Debt Instrument [Line Items]
Inception Date	[1]	March 11, 2020
Debenture conversion term		12 months
Loan Amount		$ 330,000
Outstanding balance with OID		330,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 232,810
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Four [Member]
Debt Instrument [Line Items]
Inception Date	[1]	March 13, 2020
Debenture conversion term		12 months
Loan Amount		$ 165,000
Outstanding balance with OID		165,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 60,705
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Five [Member]
Debt Instrument [Line Items]
Inception Date	[1]	March 26, 2020
Debenture conversion term		12 months
Loan Amount		$ 111,100
Outstanding balance with OID		111,100
Original Issue Discount (OID)		$ 10,100
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,100
Discount for conversion feature and warrants/shares		$ 90,900
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Six [Member]
Debt Instrument [Line Items]
Inception Date	[1]	April 8, 2020
Debenture conversion term		12 months
Loan Amount		$ 276,100
Outstanding balance with OID		276,100
Original Issue Discount (OID)		$ 25,100
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 25,000
Discount for conversion feature and warrants/shares		$ 221,654
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Seven [Member]
Debt Instrument [Line Items]
Inception Date	[1]	April 17, 2020
Debenture conversion term		12 months
Loan Amount		$ 143,750
Outstanding balance with OID		143,750
Original Issue Discount (OID)		$ 18,750
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 96,208
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Eight [Member]
Debt Instrument [Line Items]
Inception Date	[1]	April 30, 2020
Debenture conversion term		12 months
Loan Amount		$ 546,250
Outstanding balance with OID		546,250
Original Issue Discount (OID)		$ 71,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 47,500
Discount for conversion feature and warrants/shares		$ 427,500
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Twenty Nine [Member]
Debt Instrument [Line Items]
Inception Date	[1]	May 6, 2020
Debenture conversion term		12 months
Loan Amount		$ 460,000
Outstanding balance with OID		460,000
Original Issue Discount (OID)		$ 60,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 40,000
Discount for conversion feature and warrants/shares		$ 360,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty [Member]
Debt Instrument [Line Items]
Inception Date	[1]	May 18, 2020
Debenture conversion term		12 months
Loan Amount		$ 546,250
Outstanding balance with OID		221,250
Original Issue Discount (OID)		$ 46,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 35,500
Discount for conversion feature and warrants/shares		$ 439,500
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty One [Member]
Debt Instrument [Line Items]
Inception Date	[1]	June 2, 2020
Debenture conversion term		12 months
Loan Amount		$ 902,750
Outstanding balance with OID		652,750
Original Issue Discount (OID)		$ 92,750
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 58,900
Discount for conversion feature and warrants/shares		$ 708,500
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt On March 9, 2015
Debt Instrument [Line Items]
Inception Date	[1]	June 12, 2020
Debenture conversion term		12 months
Loan Amount		$ 57,500
Outstanding balance with OID		57,500
Original Issue Discount (OID)		$ 7,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,000
Discount for conversion feature and warrants/shares		$ 45,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Three [Member]
Debt Instrument [Line Items]
Inception Date	[1]	June 22, 2020
Debenture conversion term		12 months
Loan Amount		$ 138,000
Outstanding balance with OID		138,000
Original Issue Discount (OID)		$ 18,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 12,000
Discount for conversion feature and warrants/shares		$ 108,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Four [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 7, 2020
Debenture conversion term		12 months
Loan Amount		$ 586,500
Outstanding balance with OID		586,500
Original Issue Discount (OID)		$ 76,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 51,000
Discount for conversion feature and warrants/shares		$ 400,234
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Five [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 17, 2020
Debenture conversion term		12 months
Loan Amount		$ 362,250
Outstanding balance with OID		362,250
Original Issue Discount (OID)		$ 47,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 31,500
Discount for conversion feature and warrants/shares		$ 185,698
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Six [Member]
Debt Instrument [Line Items]
Inception Date	[1]	July 29, 2020
Debenture conversion term		12 months
Loan Amount		$ 345,000
Outstanding balance with OID		345,000
Original Issue Discount (OID)		$ 45,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 241,245
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Seven [Member]
Debt Instrument [Line Items]
Inception Date	[1],[4]	July 21, 2020
Debenture conversion term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 24,875
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Eight [Member]
Debt Instrument [Line Items]
Inception Date	[1]	August 14, 2020 (1)
Debenture conversion term		12 months
Loan Amount		$ 762,450
Outstanding balance with OID		462,450
Original Issue Discount (OID)		$ 69,450
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 66,300
Discount for conversion feature and warrants/shares		$ 580,124
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Thirty Nine [Member]
Debt Instrument [Line Items]
Inception Date		September 10, 2020
Debenture conversion term		12 months
Loan Amount		$ 391,000
Outstanding balance with OID		391,000
Original Issue Discount (OID)		$ 51,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 34,000
Discount for conversion feature and warrants/shares		$ 231,043
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty [Member]
Debt Instrument [Line Items]
Inception Date	[4]	September 21, 2020
Debenture conversion term		12 months
Loan Amount		$ 345,000
Outstanding balance with OID		345,000
Original Issue Discount (OID)		$ 45,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 66,375
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty One [Member]
Debt Instrument [Line Items]
Inception Date	[4]	September 23, 2020
Debenture conversion term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 20,500
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty Two [Member]
Debt Instrument [Line Items]
Inception Date	[4]	September 25, 2020
Debenture conversion term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 19,125
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty Three [Member]
Debt Instrument [Line Items]
Inception Date		December 3, 2020
Debenture conversion term		12 months
Loan Amount		$ 299,000
Outstanding balance with OID		299,000
Original Issue Discount (OID)		$ 39,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 26,000
Discount for conversion feature and warrants/shares		$ 197,882
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty Four [Member]
Debt Instrument [Line Items]
Inception Date	[4]	October 22, 2020
Debenture conversion term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 18,875
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fourty Five [Member]
Debt Instrument [Line Items]
Inception Date	[1],[4]	December 23, 2020 (1)
Debenture conversion term		6 months
Loan Amount		$ 1,000,000
Outstanding balance with OID		150,000
Original Issue Discount (OID)		$ 100,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 833,536
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Forty Fourty Six [Member]
Debt Instrument [Line Items]
Inception Date	[1]	January 5, 2021 (1)
Debenture conversion term		6 months
Loan Amount		$ 575,000
Outstanding balance with OID		575,000
Original Issue Discount (OID)		$ 75,000
Interest Rate		18.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Forty Seven [Member]
Debt Instrument [Line Items]
Inception Date		February 17, 2021
Debenture conversion term		12 months
Loan Amount		$ 230,000
Outstanding balance with OID		230,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 20,000
Discount for conversion feature and warrants/shares		$ 180,000
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Forty Eight [Member]
Debt Instrument [Line Items]
Inception Date		March 23,
Debenture conversion term		12 months
Loan Amount		$ 55,000
Outstanding balance with OID		55,000
Original Issue Discount (OID)		$ 5,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 36,431
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Forty Nine [Member]
Debt Instrument [Line Items]
Inception Date		May 24, 2021
Debenture conversion term		6 months
Loan Amount		$ 54,625
Outstanding balance with OID		54,625
Original Issue Discount (OID)		$ 7,125
Interest Rate		12.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty [Member]
Debt Instrument [Line Items]
Inception Date		May 6, 2021
Debenture conversion term		12 months
Loan Amount		$ 402,500
Outstanding balance with OID		402,500
Original Issue Discount (OID)		$ 52,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 35,000
Discount for conversion feature and warrants/shares		$ 312,551
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty One [Member]
Debt Instrument [Line Items]
Inception Date		June 17, 2021
Debenture conversion term		12 months
Loan Amount		$ 230,000
Outstanding balance with OID		230,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 20,000
Discount for conversion feature and warrants/shares		$ 144,760
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty Two [Member]
Debt Instrument [Line Items]
Inception Date		June 25, 2021
Debenture conversion term		12 months
Loan Amount		$ 977,500
Outstanding balance with OID		977,500
Original Issue Discount (OID)		$ 127,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 773,802
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty Three [Member]
Debt Instrument [Line Items]
Inception Date		May 20, 2021
Debenture conversion term		12 months
Loan Amount		$ 180,000
Outstanding balance with OID		180,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 25,824
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty Four [Member]
Debt Instrument [Line Items]
Inception Date		June 3, 2021
Debenture conversion term		12 months
Loan Amount		$ 50,000
Outstanding balance with OID		50,000
Original Issue Discount (OID)		$ 1,500
Interest Rate		12.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 7,948
Debt Instrument, Convertible, Conversion Price		$ (2.50)
Fixed Rate Convertible Notes [Member] | Convertible Debt Fifty Five [Member]
Debt Instrument [Line Items]
Inception Date		June 28, 2021
Debenture conversion term		12 months
Loan Amount		$ 350,000
Outstanding balance with OID		350,000
Original Issue Discount (OID)		$ 35,000
Interest Rate		12.00%
Conversion Price	[5]	$ 6
Deferred Finance Fees		$ 22,750
Discount for conversion feature and warrants/shares		$ 267,250
Debt Instrument, Convertible, Conversion Price	[5]	$ (6)

[1] The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.

[2] During the year ended December 31, 2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.

[3] Interest was capitalized and added to outstanding principal.

[4] The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.

[5] Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.

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Schedule of Convertible Debts and Outstanding Balances (Details) (Parenthetical) - $ / shares		6 Months Ended
	Dec. 23, 2020	Jun. 30, 2021
Debt Disclosure [Abstract]
Debt instrument, description	we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.
Debt instrument, convertible price per shares		$ 2.50
Debt instrument lowest trading price		90.00%

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Summary of Changes in Convertible Debt and Revolving Note Payable, Net of Unamortized Discounts (Details) - USD ($)	6 Months Ended	12 Months Ended
	Jun. 30, 2021	Dec. 31, 2020
Debt Disclosure [Abstract]
Balance at January 1,	$ 7,545,670
Issuance of convertible debt, face value	3,104,625
Deferred financing cost	(506,375)
Beneficial conversion feature on convertible note	(566,847)
Debt discount from shares and warrants issued with debt	(1,181,719)
Payments	(1,200,996)
Conversion of debt into equity	(150,000)
Accretion of interest and amortization of debt discount to interest expense	3,864,956
Balance at June 30,	10,909,314	$ 7,545,670
Less: current portion	10,909,314	$ 7,545,670
Convertible debt, long-term portion

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Schedule of Merchant Agreements (Details) - USD ($)	6 Months Ended	12 Months Ended
	Jun. 30, 2021	Dec. 31, 2020
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Purchase Price	$ 825,000	$ 300,000
Purchased Amount	1,125,425	413,700
Outstanding Balance	482,927	248,968
Daily Payment Rate	8,162	2,709
Deferred Finance Fees	$ 8,850
Merchant Agreements Two [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date	Feb. 04, 2021	Nov. 19, 2020
Purchase Price	$ 125,000	$ 100,000
Purchased Amount	165,000	137,900
Outstanding Balance	55,281	85,013
Daily Payment Rate	1,032	985
Deferred Finance Fees
Merchant Agreements Three [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date	Mar. 11, 2021
Purchase Price	$ 125,000
Purchased Amount	167,500
Outstanding Balance	40,559
Daily Payment Rate	1,396
Deferred Finance Fees	$ 2,500
Merchant Agreements Four [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date	Mar. 26, 2021
Purchase Price	$ 240,000
Purchased Amount	330,960
Outstanding Balance	129,952
Daily Payment Rate	2,364
Deferred Finance Fees
Merchant Agreements Five [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date	May 03, 2021
Purchase Price	$ 200,000
Purchased Amount	275,800
Outstanding Balance	141,458
Daily Payment Rate	1,970
Deferred Finance Fees	$ 5,000
Merchant Agreements Six [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date	Jun. 02, 2021
Purchase Price	$ 135,000
Purchased Amount	186,165
Outstanding Balance	115,677
Daily Payment Rate	1,400
Deferred Finance Fees	$ 1,350
Merchant Agreements One [Member]
Collaborative Arrangement and Arrangement Other than Collaborative [Line Items]
Inception Date		Nov. 05, 2020
Purchase Price		$ 200,000
Purchased Amount		275,800
Outstanding Balance		163,955
Daily Payment Rate		1,724
Deferred Finance Fees

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Convertible Debt and Other Debt (Details Narrative) - USD ($)			1 Months Ended	2 Months Ended	3 Months Ended		6 Months Ended			12 Months Ended
	Oct. 11, 2019	Oct. 01, 2019	Jun. 30, 2018	Aug. 16, 2021	Jun. 30, 2021	Jun. 30, 2020	Jun. 30, 2021	Jun. 30, 2020	Feb. 28, 2020	Dec. 31, 2020	Apr. 16, 2021	Mar. 31, 2021
Short-term Debt [Line Items]
Proceeds from Convertible Debt							$ 2,598,250	$ 4,422,600
Debt Instrument, Convertible, Beneficial Conversion Feature							566,847
Convertible Debt					$ 10,909,314		10,909,314			$ 7,545,670
Debt Instrument, Unamortized Discount					2,353,152		2,353,152			3,948,167
Debt Instrument, Face Amount					3,104,625		3,104,625
Debt Instrument, Fee Amount					8,850		8,850
Repayments of Convertible Debt							1,200,996	1,257,250
Gain (Loss) on Extinguishment of Debt					$ (498,226)	$ (1,710,151)	(1,223,385)	(2,846,518)
Repayments of Debt							$ 1,200,996
Stock Issued During Period, Shares, New Issues							56,067
Repayments of Related Party Debt							$ 153,000
Debt Instrument, Periodic Payment							$ 8,162			2,709
Small Business Administration [Member]
Short-term Debt [Line Items]
Debt Instrument, Term							5 years
Debt Instrument, Interest Rate, Stated Percentage					1.00%		1.00%
Debt Instrument, Face Amount					$ 367,038		$ 367,038
Debt Instrument, Decrease, Forgiveness							367,039
Standstill and Forbearance Agreements [Member]
Short-term Debt [Line Items]
Convertible Debt											$ 1,100,000	$ 469,000
Debt Instrument, Fee Amount					$ 1,000,000.0		$ 1,000,000.0
Standstill and Forbearance Agreements [Member] | Subsequent Event [Member]
Short-term Debt [Line Items]
Repayments of Convertible Debt				$ 827,500
Eonomic Injury Disaster Loan Program [Member]
Short-term Debt [Line Items]
Debt Instrument, Term							30 years
Debt Instrument, Interest Rate, Stated Percentage					3.75%		3.75%
Debt Instrument, Face Amount					$ 150,000		$ 150,000
Debt Instrument, Periodic Payment							731
One Lender [Member]
Short-term Debt [Line Items]
Convertible Debt					9,100,000		9,100,000
Lender [Member]
Short-term Debt [Line Items]
Convertible Debt					$ 13,300,000		$ 13,300,000
Lenders [Member]
Short-term Debt [Line Items]
Warrants and Rights Outstanding, Term					5 years		5 years
Class of Warrant or Right, Exercise Price of Warrants or Rights					$ 3.50		$ 3.50
Lenders [Member] | Standstill and Forbearance Agreements [Member]
Short-term Debt [Line Items]
Debt Instrument, Face Amount					$ 1,570,000		$ 1,570,000
Convertible Common Stock [Member]
Short-term Debt [Line Items]
Debt Instrument, Convertible, Conversion Price					$ 2.50		$ 2.50
Convertible Debt, Fair Value Disclosures					$ 1,181,719		$ 1,181,719
Minimum [Member]
Short-term Debt [Line Items]
Warrants and Rights Outstanding, Term					3 years		3 years
Class of Warrant or Right, Exercise Price of Warrants or Rights					$ 3.50		$ 3.50
Minimum [Member] | Merchant Agreements [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage					9.30%		9.30%
Maximum [Member]
Short-term Debt [Line Items]
Warrants and Rights Outstanding, Term					5 years		5 years
Class of Warrant or Right, Exercise Price of Warrants or Rights					$ 5.00		$ 5.00
Maximum [Member] | Merchant Agreements [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage					11.50%		11.50%
Convertible Debt [Member]
Short-term Debt [Line Items]
Proceeds from Convertible Debt							$ 3,104,625
Debt Instrument, Convertible, Beneficial Conversion Feature							566,847
Amortization of Debt Discount (Premium)							3,864,956
Debt Instrument, Unamortized Discount					$ 2,353,152		$ 2,353,152
Convertible Debt [Member] | Minimum [Member]
Short-term Debt [Line Items]
Debt Instrument, Term							6 months
Debt Instrument, Interest Rate, Stated Percentage					10.00%		10.00%
Convertible Debt [Member] | Maximum [Member]
Short-term Debt [Line Items]
Debt Instrument, Term							12 months
Debt Instrument, Interest Rate, Stated Percentage					18.00%		18.00%
New Loan [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage					10.00%		10.00%
Debt Instrument, Interest Rate Terms							According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications.
Gain (Loss) on Extinguishment of Debt							$ 1,600,000
Non-Convertible Loans [Member] | Private Investor [Member]
Short-term Debt [Line Items]
Debt Instrument, Term	1 month
Debt Instrument, Interest Rate, Stated Percentage	2.00%
Debt Instrument, Face Amount					$ 691,500		691,500
Proceeds from Loans	$ 25,000								$ 966,500	$ 463,500
Non-Convertible Loans [Member] | Private Investor [Member] | Warrant [Member]
Short-term Debt [Line Items]
Class of Warrant or Right, Number of Securities Called by Each Warrant or Right										150,000
Warrants and Rights Outstanding, Term										5 years
Class of Warrant or Right, Exercise Price of Warrants or Rights										$ 3.50
Repayments of Debt										$ 275,000
Fair Value Adjustment of Warrants										$ 185,660
Non-Convertible Loans [Member] | Holder [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage		10.00%
Proceeds from Loans		$ 170,000
Stock Issued During Period, Shares, New Issues		1,200
Non Convertible Loan [Member] | Private Investor [Member]
Short-term Debt [Line Items]
Debt Instrument, Term			1 year
Debt Instrument, Interest Rate, Stated Percentage			15.00%
Loans Payable			$ 15,000
Short-term Non-Convertible Loan [Member]
Short-term Debt [Line Items]
Proceeds from Short-term Debt							171,600
Repayments of Related Party Debt							$ 153,000
Short-term Non-Convertible Loan [Member] | Minimum [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage					15.00%		15.00%
Short-term Non-Convertible Loan [Member] | Maximum [Member]
Short-term Debt [Line Items]
Debt Instrument, Interest Rate, Stated Percentage					10.00%		10.00%

XML

Schedule of Concerning Options and Warrants Outstanding and Exercisable (Details)	6 Months Ended
	Jun. 30, 2021 $ / shares shares
Accumulated Other Comprehensive Income (Loss) [Line Items]
Shares, Beginning balance	15,790,603
Exercisable, Beginning balance	15,302,830
Shares, Granted	1,398,600
Shares, Exercised	(21,411)
Shares, Expired	(113,939)
Shares, Ending balance	17,053,853
Exercisable, Ending balance	16,699,039
Stock Option [Member]
Accumulated Other Comprehensive Income (Loss) [Line Items]
Shares, Beginning balance	1,355,901
Weighted average price per share, Beginning balance | $ / shares	$ 0.69
Shares, Granted	24,000
Weighted average price per share, Granted | $ / shares	$ 2.17
Shares, Exercised	(21,411)
Weighted average price per share, Exercised | $ / shares	$ 0.69
Shares, Expired	(8,444)
Weighted average price per share, Expired | $ / shares	$ 0.69
Shares, Ending balance	1,350,046
Weighted average price per share, Ending balance | $ / shares	$ 0.72
Warrant [Member]
Accumulated Other Comprehensive Income (Loss) [Line Items]
Shares, Beginning balance	14,434,702
Weighted average price per share, Beginning balance | $ / shares	$ 3.50
Shares, Granted	1,374,600
Weighted average price per share, Granted | $ / shares	$ 3.61
Shares, Exercised
Weighted average price per share, Exercised | $ / shares
Shares, Expired	(105,495)
Weighted average price per share, Expired | $ / shares	$ 3.50
Shares, Ending balance	15,703,807
Weighted average price per share, Ending balance | $ / shares	$ 3.50

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Schedule of Loans Obligated to Issue Shares (Details) - USD ($)		6 Months Ended
	Dec. 23, 2020	Jun. 30, 2021
Schedule of Capitalization, Long-term Debt [Line Items]
Loan Amount		$ 3,104,625
Debt Instrument, Description	we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.
Loans 1 [Member]
Schedule of Capitalization, Long-term Debt [Line Items]
Debt Instrument, Issuance Date		Jul. 21, 2020
Loan Amount		$ 115,000
Shares Issuable		5,000
Debt Instrument, Maturity Date		Sep. 30, 2020
Debt Instrument, Description		Monthly
Loans 2 [Member]
Schedule of Capitalization, Long-term Debt [Line Items]
Debt Instrument, Issuance Date		Sep. 21, 2020
Loan Amount		$ 345,000
Shares Issuable		12,500
Debt Instrument, Maturity Date		Nov. 16, 2020
Debt Instrument, Description		Weekly
Loans 3 [Member]
Schedule of Capitalization, Long-term Debt [Line Items]
Debt Instrument, Issuance Date		Sep. 23, 2020
Loan Amount		$ 115,000
Shares Issuable		12,500
Debt Instrument, Maturity Date		Dec. 01, 2020
Debt Instrument, Description		Weekly
Loans 4 [Member]
Schedule of Capitalization, Long-term Debt [Line Items]
Debt Instrument, Issuance Date		Sep. 25, 2020
Loan Amount		$ 115,000
Shares Issuable		12,500
Debt Instrument, Maturity Date		Dec. 01, 2020
Debt Instrument, Description		Weekly
Loans 5 [Member]
Schedule of Capitalization, Long-term Debt [Line Items]
Debt Instrument, Issuance Date		Oct. 22, 2020
Loan Amount		$ 115,000
Shares Issuable		12,500
Debt Instrument, Maturity Date		Dec. 01, 2020
Debt Instrument, Description		Weekly

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Stockholders’ Deficit (Details Narrative) - USD ($)		3 Months Ended				6 Months Ended
	Dec. 23, 2020	Jun. 30, 2021	Mar. 31, 2021	Jun. 30, 2020	Mar. 31, 2020	Jun. 30, 2021	Jun. 30, 2020	Dec. 31, 2020	Dec. 12, 2013
Class of Stock [Line Items]
Class of Warrant or Right, Number of Securities Called by Warrants or Rights		1,374,600				1,374,600
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Number		17,053,853				17,053,853		15,790,603
Share-based Payment Arrangement, Nonvested Award, Cost Not yet Recognized, Amount		$ 218,339				$ 218,339
Share-based Payment Arrangement, Nonvested Award, Cost Not yet Recognized, Period for Recognition						1 year 7 months 13 days
Share Price		$ 2.17				$ 2.17
Share-based Compensation Arrangement by Share-based Payment Award, Options, Exercisable, Intrinsic Value		$ 1,472,943				$ 1,472,943
Share-based Payment Arrangement, Option, Exercise Price Range, Outstanding, Weighted Average Exercise Price		$ 0.69				$ 0.69
Share-based Compensation Arrangement by Share-based Payment Award, Options, Exercisable, Number		16,699,039				16,699,039		15,302,830
Debt Instrument, Description	we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue					Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.
Stock Issued During Period, Shares, Restricted Stock Award, Gross							593,277
Issuance of common stock for interest paid-in-kind		$ 1,432,671	$ 2,021,780	$ 99,224		$ 3,500,000
Stock Issued During Period, Shares, Issued for Services						112,400	25,000
Stock Issued During Period, Value, Issued for Services			238,512	87,963		$ 238,512	$ 87,963
Stock Issued During Period, Shares, Conversion of Convertible Securities						139,700	420,746
Stock Issued During Period, Value, Conversion of Convertible Securities		231,250	118,000			$ 349,250	$ 1,317,649
Share-based Compensation Arrangement by Share-based Payment Award, Options, Exercises in Period						21,411
Stock Issued During Period, Shares, New Issues						56,067
Issuance of common stock for dividends paid-in-kind		$ 114,298		$ 176,748
Minimum [Member]
Class of Stock [Line Items]
Class of Warrant or Right, Exercise Price of Warrants or Rights		$ 3.50				$ 3.50
Warrants and Rights Outstanding, Term		3 years				3 years
Maximum [Member]
Class of Stock [Line Items]
Class of Warrant or Right, Exercise Price of Warrants or Rights		$ 5.00				$ 5.00
Warrants and Rights Outstanding, Term		5 years				5 years
Lenders [Member]
Class of Stock [Line Items]
Class of Warrant or Right, Number of Securities Called by Warrants or Rights		200,000				200,000
Class of Warrant or Right, Exercise Price of Warrants or Rights		$ 3.50				$ 3.50
Issuance of common stock for interest paid-in-kind, shares						10,000
Warrants and Rights Outstanding, Term		5 years				5 years
Fair value of warrants		$ 600,298				$ 600,298
Issuance of common stock for interest paid-in-kind						1,642,982
Stock Issued During Period, Value, Conversion of Convertible Securities						$ 1,700,000
Accredited Investor and Consultants [Member]
Class of Stock [Line Items]
Stock Issued During Period, Value, Restricted Stock Award, Gross							$ 1,693,251
Share-based Payment Arrangement, Option [Member]
Class of Stock [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Number		1,350,046				1,350,046
Share-based Payment Arrangement, Option, Exercise Price Range, Outstanding, Weighted Average Exercise Price		$ 0.72				$ 0.72
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Weighted Average Remaining Contractual Term						8 years 2 months 19 days
Share-based Compensation Arrangement by Share-based Payment Award, Options, Exercisable, Number		995,232				995,232
2013 Equity Incentive Plan [Member]
Class of Stock [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Number		1,350,046				1,350,046
2013 Equity Incentive Plan [Member]
Class of Stock [Line Items]
Common Stock, Capital Shares Reserved for Future Issuance									3,000,000
Series AA Preferred Stock [Member]
Class of Stock [Line Items]
Issuance of common stock for interest paid-in-kind, shares
Issuance of common stock for interest paid-in-kind
Stock Issued During Period, Shares, Issued for Services
Stock Issued During Period, Value, Issued for Services
Stock Issued During Period, Shares, Conversion of Convertible Securities
Stock Issued During Period, Value, Conversion of Convertible Securities
Stock Issued During Period, Shares, New Issues
Issuance of common stock for dividends paid-in-kind
Common Stock [Member]
Class of Stock [Line Items]
Issuance of common stock for interest paid-in-kind, shares		720,610	922,372	42,510
Issuance of common stock for interest paid-in-kind		$ 7,206	$ 9,224	$ 425
Stock Issued During Period, Shares, Issued for Services			112,400	25,000
Stock Issued During Period, Value, Issued for Services			$ 1,124	$ 250
Stock Issued During Period, Shares, Conversion of Convertible Securities		92,500	47,200
Stock Issued During Period, Value, Conversion of Convertible Securities		$ 925	$ 472
Share-based Compensation Arrangement by Share-based Payment Award, Options, Exercises in Period			21,411
Stock Issued During Period, Shares, New Issues		665,000			10,000	120,000
Issuance of common stock for dividends paid-in-kind		$ 560		$ 644
Adjustments to Additional Paid in Capital, Equity Component of Convertible Debt						$ 112,877
Securities Purchase Agreement [Member] | Series AA Preferred Stock [Member]
Class of Stock [Line Items]
Sale of Stock, Number of Shares Issued in Transaction						40
Conversion of Stock, Shares Converted						1,000
Common Stock, No Par Value		$ 0.01				$ 0.01
Sale of Stock, Consideration Received on Transaction						$ 100,000
Class of Warrant or Right, Number of Securities Called by Warrants or Rights		40,000				40,000
Class of Warrant or Right, Exercise Price of Warrants or Rights		$ 3.50				$ 3.50
Stock Options Issuance [Member]
Class of Stock [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Options, Grants in Period, Net of Forfeitures						24,000
Shares Issued, Value, Share-based Payment Arrangement, after Forfeiture						$ 49,135
Share-based Payment Arrangement, Option, Exercise Price Range, Outstanding, Weighted Average Exercise Price		$ 2.17				$ 2.17
Share-based Compensation Arrangement by Share-based Payment Award, Award Vesting Period						3 years
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Weighted Average Remaining Contractual Term						10 years
Debt Settlement [Member]
Class of Stock [Line Items]
Stock Issued During Period, Shares, Restricted Stock Award, Gross							10,000
Debt Extention And Interest Payments [Member]
Class of Stock [Line Items]
Stock Issued During Period, Shares, Restricted Stock Award, Gross							81,031
Stock Issued During Period, Value, Restricted Stock Award, Gross							$ 159,784
Settlement Accurued Liability [Member]
Class of Stock [Line Items]
Stock Issued During Period, Shares, Restricted Stock Award, Gross							66,500
Stock Issued During Period, Value, Restricted Stock Award, Gross							$ 127,855
Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		1,000,000				1,000,000
Preferred Stock, Par or Stated Value Per Share		$ 0.01				$ 0.01
Preferred Stock, Shares Issued		1,000,000				1,000,000
Series A Junior Participating Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		20,000				20,000
Series A Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		313,960				313,960
Series B Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		279,256				279,256
Series C Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		88,098				88,098
Series D Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		850				850		850
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding								300
Series E Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		500				500
Preferred Stock, Shares Outstanding		0				0
Series G Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		240,000				240,000		240,000
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding								80,570
Series H Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		10,000				10,000		10,000
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding								10,000
Series H Two Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		21				21
Series J Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		6,250				6,250		6,250
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding								3,458
Series K Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		15,000				15,000		15,000
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding								6,880
Series AA Convertible Preferred Stock [Member]
Class of Stock [Line Items]
Preferred Stock, Shares Authorized		10,000				10,000		10,000
Preferred Stock, Par or Stated Value Per Share								$ 0.01
Preferred Stock, Shares Outstanding		8,083				8,083		8,043
Common Stock Dividends, Shares							64,388
Dividends, Common Stock, Cash							$ 176,748

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Subsequent Events (Details Narrative)				3 Months Ended		6 Months Ended		12 Months Ended
	Aug. 10, 2021 USD ($) $ / shares shares	Mar. 21, 2021 USD ($) shares	Dec. 23, 2020	Jun. 30, 2021 USD ($) $ / shares shares	Mar. 31, 2020 shares	Jun. 30, 2021 USD ($) Integer $ / shares shares	Jun. 30, 2020 USD ($)	Aug. 10, 2021 USD ($) $ / shares shares	Mar. 31, 2021 USD ($)	Nov. 01, 2019 USD ($)	Jul. 19, 2019 USD ($)	Jul. 01, 2019 USD ($)	Jun. 19, 2019 USD ($)	Feb. 21, 2019 USD ($)	Nov. 13, 2018 USD ($)	Jun. 08, 2018 USD ($)
Subsequent Event [Line Items]
Proceeds from Convertible Debt						$ 2,598,250	$ 4,422,600
Stock issued to lenders | shares						56,067
Loan Amount				$ 3,104,625		$ 3,104,625
Debt insturment, description			we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue			Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lesser of $2.50 per share or 90% of the lowest trading price over the previous 20 days. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.
Number of Lenders | Integer						2
Proceeds from Issuance of Convertible Preferred Stock						$ 100,000
Repayments of Related Party Debt						153,000
Repayments of Convertible Debt						1,200,996	$ 1,257,250
Repayment line of credit						$ 300,000
Common Stock [Member]
Subsequent Event [Line Items]
Stock issued to lenders | shares				665,000	10,000	120,000
Minimum [Member]
Subsequent Event [Line Items]
Warrants and Rights Outstanding, Term				3 years		3 years
Class of Warrant or Right, Exercise Price of Warrants or Rights | $ / shares				$ 3.50		$ 3.50
Maximum [Member]
Subsequent Event [Line Items]
Warrants and Rights Outstanding, Term				5 years		5 years
Class of Warrant or Right, Exercise Price of Warrants or Rights | $ / shares				$ 5.00		$ 5.00
Convertible Loans [Member]
Subsequent Event [Line Items]
Loan Amount										$ 973,408	$ 973,408	$ 973,408	$ 973,408	$ 973,408	$ 973,408	$ 973,408
Convertible notes payable		$ 741,687							$ 167,954
Debt insturment, description		reduced the principal of another loan with a $100,000 cash payment.
Convertible Loans [Member] | Common Stock [Member]
Subsequent Event [Line Items]
Stock issued to lenders | shares		763,420
Subsequent Event [Member] | Merchant Cash Lender Agreements [Member]
Subsequent Event [Line Items]
Proceeds from Convertible Debt	$ 66,677
Repayments of Related Party Debt	62,832
Subsequent Event [Member] | Merchant Cash Lender Agreements [Member] | Chief Executive Officer [Member]
Subsequent Event [Line Items]
Repayments of Convertible Debt	$ 1,278
Subsequent Event [Member] | Series AA Convertible Preferred Stock [Member]
Subsequent Event [Line Items]
Convertible Debt On March 26, 2015 | shares	3.50							3.50
Warrants and Rights Outstanding, Term	5 years							5 years
Proceeds from Issuance of Convertible Preferred Stock	$ 900,000
Class of Warrant or Right, Number of Securities Called by Each Warrant or Right | shares	360,000							360,000
Subsequent Event [Member] | PBI Agrochem, Inc. [Member]
Subsequent Event [Line Items]
Secured debt	$ 735,000							$ 735,000
Subsequent Event [Member] | Convertible Loans [Member]
Subsequent Event [Line Items]
Proceeds from Convertible Debt	1,500,000
Repayments of Related Party Debt	$ 103,600
Subsequent Event [Member] | Convertible Loans [Member] | Minimum [Member]
Subsequent Event [Line Items]
Fixed conversion prices | $ / shares	$ 2.50							$ 2.50
Annual interest rates percentage	10.00%							10.00%
Subsequent Event [Member] | Convertible Loans [Member] | Maximum [Member]
Subsequent Event [Line Items]
Fixed conversion prices | $ / shares	$ 3.00							$ 3.00
Annual interest rates percentage	12.00%							12.00%
Subsequent Event [Member] | Fourth Convertible Loans [Member]
Subsequent Event [Line Items]
Stock issued to lenders | shares	125,000
Subsequent Event [Member] | Three Convertible Loans [Member]
Subsequent Event [Line Items]
Convertible Debt On March 26, 2015 | shares	161,000							161,000
Class of Warrant or Right, Exercise Price of Warrants or Rights | $ / shares	$ 3.50							$ 3.50
Subsequent Event [Member] | Three Convertible Loans [Member] | Minimum [Member]
Subsequent Event [Line Items]
Warrants and Rights Outstanding, Term	3 years							3 years
Subsequent Event [Member] | Three Convertible Loans [Member] | Maximum [Member]
Subsequent Event [Line Items]
Warrants and Rights Outstanding, Term	5 years							5 years
Subsequent Event [Member] | Third Convertible Loans [Member] | Minimum [Member]
Subsequent Event [Line Items]
Debt Instrument, Term								6 months
Subsequent Event [Member] | Third Convertible Loans [Member] | Maximum [Member]
Subsequent Event [Line Items]
Debt Instrument, Term	12 months