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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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 R/6a2c9IocXA7yhDhU1LPw==

<SEC-DOCUMENT>0001192482-10-000086.txt : 20100401
<SEC-HEADER>0001192482-10-000086.hdr.sgml : 20100401
<ACCEPTANCE-DATETIME>20100401150445
ACCESSION NUMBER:		0001192482-10-000086
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20100401
DATE AS OF CHANGE:		20100401
EFFECTIVENESS DATE:		20100401

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			PRESSURE BIOSCIENCES INC
		CENTRAL INDEX KEY:			0000830656
		STANDARD INDUSTRIAL CLASSIFICATION:	MEASURING & CONTROLLING DEVICES, NEC [3829]
		IRS NUMBER:				042652826
		STATE OF INCORPORATION:			MA
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-83065-6B
		FILM NUMBER:		10723636

	BUSINESS ADDRESS:	
		STREET 1:		14 NORFOLK AVENUE
		CITY:			SOUTH EASTON
		STATE:			MA
		ZIP:			02375
		BUSINESS PHONE:		5085801818

	MAIL ADDRESS:	
		STREET 1:		14 NORFOLK AVENUE
		CITY:			SOUTH EASTON
		STATE:			MA
		ZIP:			02375

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	BOSTON BIOMEDICA INC
		DATE OF NAME CHANGE:	19960812
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0000830656</cik>
        <entityName>PRESSURE BIOSCIENCES INC</entityName>
        <issuerAddress>
            <street1>14 NORFOLK AVENUE</street1>
            <city>SOUTH EASTON</city>
            <stateOrCountry>MA</stateOrCountry>
            <zipCode>02375</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>5085801818</issuerPhoneNumber>
        <jurisdictionOfInc>MASSACHUSETTS</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>BOSTON BIOMEDICA INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Richard</firstName>
                <middleName>T</middleName>
                <lastName>Schumacher</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>14 Norfolk Avenue</street1>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Edmund</firstName>
                <middleName>Y</middleName>
                <lastName>Ting</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>14 Norfolk Avenue</street1>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Nathan</firstName>
                <middleName>P</middleName>
                <lastName>Lawrence</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>14 Norfolk Avenue</street1>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Alexander</firstName>
                <lastName>Lazarev</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>14 Norfolk Avenue</street1>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Matthew</firstName>
                <middleName>B</middleName>
                <lastName>Potter</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>14 Norfolk Avenue</street1>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>R</firstName>
                <middleName>Wayne</middleName>
                <lastName>Fritzsche</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Pressure BioSciences, Inc.</street1>
                <street2>14 Norfolk Avenue</street2>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Calvin</firstName>
                <middleName>A</middleName>
                <lastName>Saravis</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Pressure BioSciences, Inc.</street1>
                <street2>14 Norfolk Avenue</street2>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>J</firstName>
                <middleName>Donald</middleName>
                <lastName>Payne</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Pressure BioSciences, Inc.</street1>
                <street2>14 Norfolk Avenue</street2>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Alan</firstName>
                <lastName>Rosenson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Pressure BioSciences, Inc.</street1>
                <street2>14 Norfolk Avenue</street2>
                <city>South Easton</city>
                <stateOrCountry>MA</stateOrCountry>
                <zipCode>02375</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$1,000,001 - $5,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001192482-09-000305</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-11-18</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Lantern Investments, Inc.</recipientName>
                <recipientCRDNumber>30996</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>35 Pinelawn Road, Suite 101E</street1>
                    <city>Melville</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <zipCode>11747</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <state>CO</state>
                    <state>FL</state>
                    <state>MI</state>
                    <state>MN</state>
                    <state>NY</state>
                    <state>OK</state>
                    <state>SD</state>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>2500000</totalOfferingAmount>
            <totalAmountSold>1667766</totalAmountSold>
            <totalRemaining>832234</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>31</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>100478</dollarAmount>
            </findersFees>
            <clarificationOfResponse>In addition, the finder received warrants to purchase an aggregate of 5,344 shares of Series B Convertible Preferred Stock at $28.80 per share, which warrants expire on August 11, 2012.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>PRESSURE BIOSCIENCES INC</issuerName>
                <signatureName>/s/ Richard T. Schumacher</signatureName>
                <nameOfSigner>Richard T. Schumacher</nameOfSigner>
                <signatureTitle>President and CEO</signatureTitle>
                <signatureDate>2010-03-31</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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