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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
 MFgwCgYEVQgBAQICAf8DSgAwRwJAW2sNKK9AVtBzYZmr6aGjlWyK3XmZv3dTINen
 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 P/JYwg62uC+8cjCxnfQ056n8olpg52nERX6NRDXltB8a0tubbAy52ufMLhRUvN3B
 qh2swER2n+maXkENjOwx6Q==

<SEC-DOCUMENT>0001179110-08-015082.txt : 20080812
<SEC-HEADER>0001179110-08-015082.hdr.sgml : 20080812
<ACCEPTANCE-DATETIME>20080812134611
ACCESSION NUMBER:		0001179110-08-015082
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20080811
FILED AS OF DATE:		20080812
DATE AS OF CHANGE:		20080812

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			FASTENAL CO
		CENTRAL INDEX KEY:			0000815556
		STANDARD INDUSTRIAL CLASSIFICATION:	RETAIL-BUILDING MATERIALS, HARDWARE, GARDEN SUPPLY [5200]
		IRS NUMBER:				410948415
		STATE OF INCORPORATION:			MN
		FISCAL YEAR END:			1231

	BUSINESS ADDRESS:	
		STREET 1:		2001 THEURER BLVD
		CITY:			WINONA
		STATE:			MN
		ZIP:			55987
		BUSINESS PHONE:		5074545374

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			OBERTON WILLARD D
		CENTRAL INDEX KEY:			0001036295

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	000-16125
		FILM NUMBER:		081009150

	BUSINESS ADDRESS:	
		BUSINESS PHONE:		5074545374

	MAIL ADDRESS:	
		STREET 1:		2001 THEURER BLVD
		CITY:			WINONA
		STATE:			MN
		ZIP:			55987
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>edgar.xml
<DESCRIPTION>FORM 4 -
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0303</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2008-08-11</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0000815556</issuerCik>
        <issuerName>FASTENAL CO</issuerName>
        <issuerTradingSymbol>FAST</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001036295</rptOwnerCik>
            <rptOwnerName>OBERTON WILLARD D</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>2001 THEURER BOULEVARD</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>WINONA</rptOwnerCity>
            <rptOwnerState>MN</rptOwnerState>
            <rptOwnerZipCode>55987</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>CEO and President</officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2008-08-11</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>25000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>52.4279</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>232440</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable></derivativeTable>

    <footnotes>
        <footnote id="F1">An additional 71600 shares of issuer's common stock are owned by the reporting person's spouse.  The reporting person disclaims beneficial ownership of such shares.</footnote>
        <footnote id="F2">An additional 6400 shares of issuer's common stock are held by the reporting person and reporting person's spouse as custodian for their minor children.  The reporting person disclaims beneficial ownership of such shares.</footnote>
        <footnote id="F3">The reporting person indirectly owns 110 shares of issuer's common stock through the issuer's 401(K) plan as of December 31, 2007.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ John Milek, Attorney-in-Fact</signatureName>
        <signatureDate>2008-08-12</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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