EXHIBIT 23.1

EXHIBIT NO. 23.1

Consent of Independent Registered Public Accounting Firm

We hereby consent to the incorporation by reference in the Registration Statements on Forms S-3 (No. 333-237169 and No. 333-260498) and Forms S-8 (No. 333-226984, No. 333-236721 and No 333-238752) of Colony Bankcorp, Inc. of our report dated March 18, 2022 relating to the consolidated financial statements, appearing in this Annual Report on Form 10-K of Colony Bankcorp, Inc. for the year ended December 31, 2021.

/s/ Mauldin & Jenkins., LLC

Albany, Georgia

March 18, 2022