-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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 obl2xa1egkkMYaUw4XGZww==

<SEC-DOCUMENT>0000708717-09-000002.txt : 20091102
<SEC-HEADER>0000708717-09-000002.hdr.sgml : 20091102
<ACCEPTANCE-DATETIME>20091102155919
ACCESSION NUMBER:		0000708717-09-000002
CONFORMED SUBMISSION TYPE:	D
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20091102
DATE AS OF CHANGE:		20091102
EFFECTIVENESS DATE:		20091102

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			ALFACELL CORP
		CENTRAL INDEX KEY:			0000708717
		STANDARD INDUSTRIAL CLASSIFICATION:	BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
		IRS NUMBER:				222369085
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			0731

	FILING VALUES:
		FORM TYPE:		D
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-70871-7N
		FILM NUMBER:		091151166

	BUSINESS ADDRESS:	
		STREET 1:		300 ATRIUM DRIVE
		CITY:			SOMERSET
		STATE:			NJ
		ZIP:			08873
		BUSINESS PHONE:		732-652-4525

	MAIL ADDRESS:	
		STREET 1:		300 ATRIUM DRIVE
		CITY:			SOMERSET
		STATE:			NJ
		ZIP:			08873
</SEC-HEADER>
<DOCUMENT>
<TYPE>D
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0000708717</cik>
        <entityName>ALFACELL CORP</entityName>
        <issuerAddress>
            <street1>300 ATRIUM DRIVE</street1>
            <city>SOMERSET</city>
            <stateOrCountry>NJ</stateOrCountry>
            <zipCode>08873</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>732-652-4525</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Charles</firstName>
                <lastName>Muniz</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>David</firstName>
                <lastName>Sidransky</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John</firstName>
                <middleName>P.</middleName>
                <lastName>Brancaccio</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Stephen</firstName>
                <middleName>K.</middleName>
                <lastName>Carter</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Donald</firstName>
                <middleName>R.</middleName>
                <lastName>Conklin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Paul</firstName>
                <middleName>M.</middleName>
                <lastName>Weiss</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kuslima</firstName>
                <lastName>Shogen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>c/o Alfacell Corp., 300 Atrium Drive</street1>
                <city>Somerset</city>
                <stateOrCountry>NJ</stateOrCountry>
                <zipCode>08873</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2009-10-19</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>3250000</totalOfferingAmount>
            <totalAmountSold>3250000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>8</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>Proceeds from the offering will be used for general working capital.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>ALFACELL CORP</issuerName>
                <signatureName>/s/ Charles Muniz</signatureName>
                <nameOfSigner>Charles Muniz</nameOfSigner>
                <signatureTitle>President, CEO, CFO</signatureTitle>
                <signatureDate>2009-10-28</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----