SEC Form 4

FORM 4		UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940	OMB APPROVAL OMB Number: 3235-0287 Estimated average burden hours per response: 0.5
	Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

1. Name and Address of Reporting Person* BANK OF AMERICA VENTURES (Last) (First) (Middle) 950 TOWER LANE, SUITE 700 (Street) FOSTER CITY CA 94404 (City) (State) (Zip)	2. Issuer Name and Ticker or Trading Symbol ACUSPHERE INC [ acus ]	5. Relationship of Reporting Person(s) to Issuer (Check all applicable) Director X 10% Owner Officer (give title below) Other (specify below)
	3. Date of Earliest Transaction (Month/Day/Year) 04/01/2004
	4. If Amendment, Date of Original Filed (Month/Day/Year)	6. Individual or Joint/Group Filing (Check Applicable Line) Form filed by One Reporting Person X Form filed by More than One Reporting Person

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1. Title of Security (Instr. 3)	2. Transaction Date (Month/Day/Year)	2A. Deemed Execution Date, if any (Month/Day/Year)	3. Transaction Code (Instr. 8)		4. Securities Acquired (A) or Disposed Of (D) (Instr. 3, 4 and 5)			5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4)	6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	7. Nature of Indirect Beneficial Ownership (Instr. 4)
			Code	V	Amount	(A) or (D)	Price
Common stock								1,314,146	D
Common stock								146,016	I	By Banc of America Partners II(1)
Common stock	04/01/2004		J(2)		452,414	A	$0(2)	452,414	I	By BancBoston Ventures Inc.(3)
Common stock	04/05/2004		J(4)		6,521	A	$0(5)	6,521	I	By Bank of America Capital Corporation(5)

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security (Instr. 3)	2. Conversion or Exercise Price of Derivative Security	3. Transaction Date (Month/Day/Year)	3A. Deemed Execution Date, if any (Month/Day/Year)	4. Transaction Code (Instr. 8)		5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5)		6. Date Exercisable and Expiration Date (Month/Day/Year)		7. Title and Amount of Securities Underlying Derivative Security (Instr. 3 and 4)		8. Price of Derivative Security (Instr. 5)	9. Number of derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4)	10. Ownership Form: Direct (D) or Indirect (I) (Instr. 4)	11. Nature of Indirect Beneficial Ownership (Instr. 4)
				Code	V	(A)	(D)	Date Exercisable	Expiration Date	Title	Amount or Number of Shares

1. Name and Address of Reporting Person* BANK OF AMERICA VENTURES (Last) (First) (Middle) 950 TOWER LANE, SUITE 700 (Street) FOSTER CITY CA 94404 (City) (State) (Zip) Relationship of Reporting Person(s) to Issuer Director X 10% Owner Officer (give title below) Other (specify below)

1. Name and Address of Reporting Person* BANK OF AMERICA CORP /DE/ (Last) (First) (Middle) BANK OF AMERICA CORPORATE CENTER 100 N TRYON ST (Street) CHARLOTTE NC 28255 (City) (State) (Zip) Relationship of Reporting Person(s) to Issuer Director X 10% Owner Officer (give title below) Other (specify below)

1. Name and Address of Reporting Person* BANK OF AMERICA NA (Last) (First) (Middle) 110 N TYRONE (Street) CHARLOTTE NC 28255 (City) (State) (Zip) Relationship of Reporting Person(s) to Issuer Director X 10% Owner Officer (give title below) Other (specify below)

1. Name and Address of Reporting Person* NB HOLDINGS CORP (Last) (First) (Middle) 100 NORTH TRYON STREET (Street) CHARLOTTE NC 28255 (City) (State) (Zip) Relationship of Reporting Person(s) to Issuer Director X 10% Owner Officer (give title below) Other (specify below)

Explanation of Responses:

1. Held by BA Venture Partners II, with which the Reporting Persons have an employment releationship. The Reporting Persons disclaim beneficial ownership of such securities.

2. On April 1, 2004, Bank of America Corporation, one of the Reporting Persons, merged with FleetBoston Financial Corporation. As a result of such merger, Bank of America corporation may be deemed to have a pecuniary interest in shares of the Issuer held by BancBoston Ventures, Inc., an indirect wholly-owned subsidiary of FleetBoston Financial Corporation. The Reporting Persons disclaim beneficial ownership of such securities.

3. Held by BancBoston Ventures, Inc. The Reporting Persons disclaim beneficial ownership of such securities.

4. On April 5, 2004, these securities were distributed to Bank of America Capital Corporation, an affiliate of Reporting Persons. Such shares were distributed (for no additional consideration) to Bank of America Capital Corporation by the general partner of a limited partnership of which Bank of America Capital Corporation is a limited partner.

5. Held by Bank of America Capital Corporation. The Reporting Persons disclaim beneficial ownership of such securities.

	/s/ Kate Mitchell, By: Kate Mitchell, President and Managing Director, Bank of America Ventures	04/05/2004
	/s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, Bank of America Corporation	04/05/2004
	/s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, Bank of America, N.A.	04/05/2004
	/s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, NB Holdings Corporation	04/05/2004
	** Signature of Reporting Person	Date
Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
* If the form is filed by more than one reporting person, see Instruction 4 (b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
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