Form of

JANUS INVESTMENT FUND

Janus Fund              Janus Twenty Fund

Special Meeting of Shareholders

adjourned to April 18, 2017

Urgent Request   PLEASE SUBMIT YOUR VOTE TODAY!   Dear Shareholder:   The Special Meeting of Shareholders scheduled for April 6, 2017 has been adjourned to April 18, 2017 at 10:00 a.m. Mountain time. To date, our records indicate that we have not yet received your vote. If you have already voted, thank you. If you have not yet voted, please submit your vote promptly.   For the reasons set forth in the proxy materials, the Board of Trustees recommends that you vote “FOR” the proposals. Your vote is important no matter how many shares you own. We urge you to act promptly in order to allow us to obtain a sufficient number of votes to hold the meeting and avoid the cost of additional solicitation.   If you have any questions regarding the proposals, or need duplicate proxy materials, please contact Computershare Fund Services at 1-866-492-0863. Representatives are available Monday through Friday from 9:00 a.m. to 11:00 p.m. and Saturday from 12:00 p.m. to 6:00 p.m. Eastern time. A copy of the proxy statement is also available on Computershare’s website at www.proxy-direct.com/jif-28315.       For your convenience, please utilize any of the following methods to submit your vote:   1. By Internet. Log on to the website noted on your proxy card(s) or voting instruction form, enter the control number printed on the voting card(s), and follow the simple online instructions.   2. By Touch-Tone Phone. Call the toll-free number found on your voting card(s) or voting instruction form, and follow the simple instructions.   3. By Speaking to a Proxy Specialist. Call 1-866-492-0863 to speak to a live proxy specialist. We can answer your questions and record your vote.   4. By Mail. Simply return your signed and dated voting card(s) or voting instruction form in the envelope provided.   5. Attend the Special Meeting of Shareholders on April 18, 2017.   Please utilize one of the options above to vote your shares, so your vote can be received in time for the meeting.  

YOUR VOTE IS VERY IMPORTANT. PLEASE VOTE YOUR SHARES TODAY!

ADJ1_28315_Janus

Fund/Janus Twenty