JOHN HANCOCK INVESTMENT TRUST

200 Berkeley Street

Boston, Massachusetts 02116

January 14, 2021

VIA EDGAR TRANSMISSION

U.S. Securities and Exchange Commission

100 F Street, NE

Washington, D.C. 20549

Re: John Hancock Investment Trust (the “Trust”), on behalf of: John Hancock ESG Large Cap Core Fund; Registration Statement on Form N-14

Ladies and Gentlemen:

Enclosed for filing on behalf of the Trust, we are filing today a registration statement on Form N-14 (the “Registration Statement”) by electronic submission via EDGAR relating to the issuance of shares in connection with the reorganization of John Hancock ESG All Cap Core Fund into John Hancock ESG Large Cap Core Fund, each a series of the Trust.

The Registration Statement is proposed to become effective on February 19, 2021 pursuant to Rule 488 under the Securities Act of 1933, as amended.

No fees are required in connection with this filing. If you have any questions or comments concerning the foregoing, please contact Abigail Hemnes at Abigail.Hemnes@klgates.com or at (508) 284-8235, or Sarah Coutu at Sarah_Coutu@jhancock.com or at (617) 816-2859.

Sincerely,

/s/ Sarah M. Coutu

Sarah M. Coutu

Assistant Secretary of the Trust