Exhibit 23.1

Independent Registered Public Accounting Firm’s Consent

We consent to the inclusion in this Registration Statement of American Acquisition Opportunity Inc. (the “Company”) on Form S-1 Amendment No. 1 [File No. 333-252571] of our report dated February 4, 2021, which includes an explanatory paragraph as to the Company’s ability to continue as going concern, with respect to our audit of the financial statements of American Acquisition Opportunity Inc. as of January 22, 2021 and for the period from January 20, 2021 (inception) through January 22, 2021, which report appears in the Prospectus, which is part of this Registration Statement. We also consent to the reference to our Firm under the heading “Experts” in such Prospectus.

/s/ Marcum llp

Marcum llp

New York, NY

March 9, 2021