Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the reference to our firm under the caption "Experts" and to the use of our report dated March 14, 2022, in the in the Registration Statement (Form S-1) and related Prospectus of Sema4 Holdings Corp. for the registration of shares its Class A common stock.

/s/ Ernst & Young LLP

New York, NY

May 2, 2022