Exhibit 99.5
June 30, 2020
To: | Max Munn |
SteriLumen | |
50 N. Macquesten Parkway | |
Mount Vernon, NY 10550 |
From: | Matthew Hardwick, PhD |
ResInnova Laboratories | |
8807 Colesville Rd, 3rd Floor | |
Silver Spring, MD 20910 |
Dear Mr. Munn,
Our laboratory tested both the mirror and drain units against OC43 human coronavirus. The mirror unit demonstrated a 4.56-log kill after a 2 hour exposure and the drain unit demonstrated a 1.56-log kill after a 15 min exposure. Please find the corresponding report in the following pages. OC43 is a common surrogate for SARS CoV-2, the cause of COVID-19. Moreover, both are beta coronaviruses. Therefore, it is expected that SARS CoV-2, the virus that causes COVID-19, will be killed in a similar manner to OC43.
Sincerely,
/s/ Matthew Hardwick
Matthew Hardwick, PhD
President/CEO
ResInnova Laboratories
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June 30, 2020 | |||||
Antiviral Evaluation | |||||
Test Method Details | |||||
Test Method | Modified ASTM E2197 | ||||
Test Organism | OC43 human coronavirus | ||||
Test Solution | Eagles Modified Medium with 2% Fetal Bovine Serum | ||||
Test Disks | 20mm diameter magnetic stainless steel disk | ||||
Inoculum Applied to Test Disk | 0.010 mL applied to each disk and spread to within 1 mm of edges, then dried at room temperature | ||||
Recovery Solution | Modified SDLP Buffer | ||||
Measuring Method of Number of Viable Viral Particles | Dilution Plate Method onto RD cells, viral particles were | ||||
determined using an anti-OC43 monoclonal antibody. Foci | |||||
were then counted giving Foci Forming Units (FFU). | |||||
Results | |||||
Modified ASTM E2197: Standard Quantitative Disk Carrier Test Method | |||||
Number of Replicate Experiments | 1 | ||||
Average Control Recovery ± S.E.M. (FFU/mL) | 3.62E+04 ± 0.69E+04 = 4.55 ± 3.84 log | ||||
Average Virus | Average | Average | Average Percent | ||
Location | Recovered (FFU/ | Log Recovery | Log Reduction (vs | Reduction (vs | |
disk) | Control) | Control) | |||
Sink Backsplash | 0 | NA | 4.56 | >99.99% | |
Sink Edge | 0 | NA | 4.56 | >99.99% | |
Sink Drain | 0 | NA | 4.56 | >99.99% | |
Drainpipe 1 | 1.00E+3 | 3.00 | 1.56 | 97.24% |
Figure 1: Test Unit Setup
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Figure 2: Sample Sites for the Mirror Unit (left) and Drainpipe Unit (right)
Figure 3: Anti-coronavirus (OC43) results for each test location
ResInnova Laboratories
8807 Colesville Rd, 3rd Floor
Silver Spring, MD 20910