SC 13G/A

Names of Reporting Person:

Syncona Portfolio Limited

Check the appropriate box if a member of a Group (see instructions)

(a) ☐ (b) ☒

SEC Use Only

Citizenship or Place of Organization

Guernsey

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 13G

Under the Securities Exchange Act of 1934

(Amendment No. 1)*

Freeline Therapeutics Holdings plc

(Name of Issuer)

American Depositary Shares and Ordinary Shares, nominal value £0.00001 per share

(Title of Class of Securities)

35655L 107**

(CUSIP Number)

December 31, 2021

(Date of Event which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

☐ Rule 13d-1(b)

☐ Rule 13d-1(c)

☒ Rule 13d-1(d)

*	The remainder of this cover page shall be filled out for a reporting person’s initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be deemed to be “filed” for the purpose of Section 18 of the Securities Exchange Act of 1934 (“Act”) or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

There is no CUSIP number assigned to the ordinary shares. CUSIP number 35655L 107 has been assigned to the American Depositary Shares of the Issuer, which are quoted on the Nasdaq Global Select Market under the symbol “FRLN.” Each American Depositary Share represents the right to receive one ordinary share.

SCHEDULE 13G


CUSIP No. 35655L 107

(1)

Consists of 15,864,527 ordinary shares and 2,950,000 American Depositary Shares (“ADSs”) held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer. The ordinary shares and the ADS are collectively referred to as the “Ordinary Shares”.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the Securities and Exchange Commission (the “SEC”) on November 9, 2021.

Page 2 of 11

SCHEDULE 13G


CUSIP No. 35655L 107

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

Page 3 of 11

SCHEDULE 13G


CUSIP No. 35655L 107

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

Page 4 of 11

SCHEDULE 13G


CUSIP No. 35655L 107

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

Page 5 of 11

SCHEDULE 13G


CUSIP No. 35655L 107

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

Page 6 of 11

SCHEDULE 13G


CUSIP No. 35655L 107

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(2)	Based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

Page 7 of 11

Item 1.

(a) Name of Issuer: Freeline Therapeutics Holdings plc

(b) Address of Issuer’s principal executive offices: Stevenage Bioscience Catalyst, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2FX, United Kingdom

Item 2.

(a)

Name of reporting persons filing:

(i)

Syncona Portfolio Limited;

(ii)

Syncona Holdings Limited;

(iii)

Syncona Investment Management Limited;

(iv)

Syncona Limited;

(v)

Martin Murphy; and

(vi)

Chris Hollowood

(b)

Address of principal business office or, if none, residence:

The address of the principal business office of Syncona Portfolio Limited, Syncona Holdings Limited and Synocna Limited is Arnold House, St Julian’s Avenue, St Peter Port, Guernsey GY1 3RD, Channel Islands. The address of the principal business office of Syncona Investment Management Limited, Martin Murphy and Chris Hollowood is 2nd Floor, 8 Bloomsbury Street, London WC1B 3SR, United Kingdom.

(c)

Citizenship:

Syncona Portfolio Limited, Syncona Holdings Limited and Syncona Limited’s citizenship is Guernsey. Syncona Investment Management Limited, Martin Murphy and Chris Hollowood’s citizenship is United Kingdom.

(d)

Title and class of securities:

Ordinary Shares, nominal value £0.00001 per share, and American Depositary Shares (“ADS”). Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer.

(e)

CUSIP No.:

There is no CUSIP number assigned to the ordinary shares. CUSIP number 35655L 107 has been assigned to the ADS, which are quoted on the Nasdaq Global Select Market under the symbol “FRLN.”

Item 3.

If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:

Not applicable.

Page 8 of 11

Item 4.

The following information with respect to the ownership of Ordinary Shares of the Issuer by the Reporting Persons filing this statement on Schedule 13G is provided as of December 31, 2021:


Reporting Persons	Ordinary Shares Held Directly (1)		Sole Power to Vote or Direct the Vote (1)		Shared Power to Vote or Direct the Vote (1)		Sole Power to Dispose or Direct the Disposition (1)		Shared Power to Dispose or Direct the Disposition (1)		Amount Beneficially Owned (1)		Percentage of Class (2)
Syncona Portfolio Limited		18,814,527		0		18,814,527		0		18,814,527		18,814,527		52.5	%
Syncona Holdings Limited		0		0		18,814,527		0		18,814,527		18,814,527		52.5	%
Syncona Investment Management Limited		0		0		18,814,527		0		18,814,527		18,814,527		52.5	%
Syncona Limited		0		0		18,814,527		0		18,814,527		18,814,527		52.5	%
Martin Murphy		0		0		18,814,527		0		18,814,527		18,814,527		52.5	%
Chris Hollowood		0		0		18,814,527		0		18,814,527		18,814,527		52.5	%

(1)	Consists of 15,864,527 ordinary shares and 2,950,000 ADS held directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value £0.00001 per share, of the Issuer. The ordinary shares and the ADS are collectively referred to as the “Ordinary Shares”.

(2)	The percentage of outstanding Ordinary Shares of the Issuer beneficially owned was calculated based on 35,814,255 Ordinary Shares outstanding as of September 30, 2021 as reported in the Issuer’s report on Form 6-K filed with the SEC on November 9, 2021.

The shares are owned directly by Syncona Portfolio Limited (“Syncona Portfolio”) and indirectly by Syncona Holdings Limited (“Syncona Holdings”), Syncona Limited (“Syncona Limited”), Martin Murphy and Chris Hollowood. Syncona Portfolio is a wholly owned subsidiary of Syncona Holdings, and Syncona Holdings is a wholly controlled subsidiary of Syncona Limited (“Syncona Limited”), a publicly-listed company. Investment and voting decisions with respect to these shares are made by Syncona Portfolio, acting upon the recommendation of Martin Murphy and Chris Hollowood, who comprise the investment committee of Syncona Investment Management Limited, also a subsidiary of Syncona Holdings. Each of these entities disclaims beneficial ownership except to the extent of its pecuniary interest therein, if any.

Item 5.

Ownership of Five Percent or Less of a Class.

If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than five percent of the class of securities, check the following ☐.

Item 6.

Ownership of more than Five Percent on Behalf of Another Person.

Not applicable.

Item 7.

Identification and classification of the subsidiary which acquired the security being reported on by the parent holding company or control person.

Not applicable.

Item 8.

Identification and classification of members of the group.

Not applicable.

Item 9.

Notice of Dissolution of Group.

Not applicable.

Item 10.

Certifications.

Not applicable.

Page 9 of 11

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

Dated: February 11, 2022


SYNCONA PORTFOLIO LIMITED

By:		/s/ Rob Hutchinson
Name:		Rob Hutchinson
Title:		Director

SYNCONA HOLDINGS LIMITED

By:		/s/ Rob Hutchinson
Name:		Rob Hutchinson
Title:		Director

SYNCONA INVESTMENT MANAGEMENT LIMITED

By:		/s/ Martin Murphy
Name:		Martin Murphy
Title:		CEO

SYNCONA LIMITED

By:		/s/ Rob Hutchinson
Name:		Rob Hutchinson
Title:		Director

		/s/ Martin Murphy
		Martin Murphy

		/s/ Chris Hollowood
		Chris Hollowood

Exhibit(s):


Exhibit 99.1:		Joint Filing Statement