EXHIBIT 16.1

U.S. Securities and Exchange Commission Office of the Chief Accountant 100 F Street Northeast Washington, DC 20549 RE: CDT Environmental Technology Investment Holdings Limited CIK: 0001793895 We have been furnished a copy of the statements being made by CDT Environmental Technology Investment Holdings Limited (the “Company”) in its Form 6-K dated April 26, 2024 and captioned “Change in Registrant’s Certifying Accountant.” We acknowledge that Wei, Wei & Co., LLP had issued audit reports on the consolidated financial statements of the Company for the years ended December 31, 2021 and 2022. During the period from April 18, 2022, the date of our appointment as the Company’s independent registered public accounting firm, through April 25, 2024, the date of our dismissal, there were no disagreements between us and the Company regarding accounting principles that we need to make reference to. We agree with all statements pertaining to us in such Form 6-K. We have no basis to agree or disagree with any other statements of the Registrant contained in Exhibit 16.1. Sincerely, /s/ Wei, Wei & Co., LLP Wei, Wei & Co., LLP