Exhibit 23.1

Independent Registered Public Accounting Firm’s Consent

We consent to the incorporation by reference in this Registration Statement of Longeveron Inc. on Amendment No. 1 to Form S-1 (File No. 333-278073) of our report dated February 27, 2024, which includes an explanatory paragraph as to the Company’s ability to continue as a going concern, with respect to our audits of the financial statements of Longeveron Inc. as of December 31, 2023 and 2022 and for the years ended December 31, 2023 and 2022, appearing in the Annual Report on Form 10-K of Longeveron Inc. for the year ended December 31, 2023. We also consent to the reference to our Firm under the heading “Experts” in the Prospectus, which is part of the Registration Statement.

/s/ Marcum LLP

Marcum llp

Hartford, CT

April 3, 2024