Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Forms S-8 (No. 333-254023, 333-237044, 333-230129 and 333-224403) and Form S-3 (No. 333-258518 and 333-231144) of Surface Oncology, Inc. of our report dated March 2, 2022 relating to the financial statements, which appears in this Form 10-K.

/s/ PricewaterhouseCoopers LLP

Boston, Massachusetts

March 2, 2022

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