Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on Form S-11 (No. 333-252077) of Nuveen Global Cities REIT, Inc. of our report dated March 24, 2023 relating to the financial statements and financial statement schedule, which appears in Nuveen Global Cities REIT, Inc.’s Annual Report on Form 10-K for the year ended December 31, 2022. We also consent to the reference to us under the heading “Experts” in such Registration Statement.

/s/ PricewaterhouseCoopers LLP

Charlotte, North Carolina

April 17, 2023