Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in this Registration Statement on Form S-8 dated January 22, 2021, of our report dated March 10, 2020, with respect to the consolidated financial statements of Krystal Biotech, Inc. as of December 31, 2019 and 2018, and the related statements of operations and comprehensive loss, stockholders’ equity and cash flows for each of the two years in the period ended December 31, 2019.

/s/ Mayer Hoffman McCann P.C.

San Diego, California

January 22, 2021