Exhibit 21.1

List of Subsidiaries of GitLab Inc.

(including place of incorporation)

GitLab Federal, LLC – (United States)

GitLab GK – (Japan)

GitLab Korea Limited – (South Korea)

GitLab B.V. – (Netherlands)

GitLab IT B.V. – (Netherlands)

GitLab UK Limited – (United Kingdom)

GitLab GmbH – (Germany)

GITLAB PTY LTD – (Australia)

GITLAB CANADA CORP. – (Canada)

GitLab France – (France)

GITLAB IRELAND LIMITED – (Ireland)

GitLab Services Inc. – (United States)

GITLAB SINGAPORE HOLDING PTE. LTD. – (Singapore)

GITLAB SINGAPORE PTE. LTD. – (Singapore)

GitLab Information Technology (Hubei) Co., Ltd. – (China)

JiHu Innovation (Beijing) Information Technology Co., Ltd - (China)

JiHu GitLab Technology Limited - (Hong Kong)

JiHu Information Technology (Chongqing) Co., Ltd - (China)

GitLab Iberia, S.L. - (Spain)

GitLab India Private Limited - (India)