PROSPECTUS SUPPLEMENT NO. 7 (TO PROSPECTUS DATED OCTOBER 8, 2020) Filed Pursuant to Rule 424(b)(3) Registration No. 333-245405

200,000 Units, Each Consisting of

One 7.75% Series A Cumulative Convertible Preferred Share and

Eight Warrants, Each Warrant Exercisable for One Common

Share for a Total of up to 1,600,000 Common Shares Underlying the Warrants

Pyxis Tankers Inc.

This is a supplement (“Prospectus Supplement”) to the prospectus, dated October 8, 2020 (“Prospectus”) of Pyxis Tankers Inc. (the “Company”), which forms a part of the Company’s Registration Statement on Form F-1 (No. 333-245405).

On July 12, 2021, the Company filed on Form 6-K with the U.S. Securities and Exchange Commission as set forth below.

This Prospectus Supplement should be read in conjunction with, and delivered with, the Prospectus and is qualified by reference to the Prospectus except to the extent that the information in this Prospectus Supplement supersedes the information contained in the Prospectus.

This Prospectus Supplement is not complete without, and may not be delivered or utilized except in connection with, the Prospectus, including any amendments or supplements to it.

Our common shares, 7.75% Series A Cumulative Convertible Preferred Shares, and Warrants are listed on the Nasdaq Capital Market under the symbols “PXS,” “PXSAP” and “PSXAW,” respectively.

Investing in our securities is highly speculative and involves a high degree of risk. See “Risk Factors” beginning on page 21 of this prospectus for a discussion of information that should be considered in connection with an investment in our securities and in the documents incorporated by reference into this prospectus.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved these securities, or determined if this prospectus is truthful or complete. Any representation to the contrary is a criminal offense.

The date of this prospectus is July 29, 2021