Issuer Free Writing Prospectus

Filed Pursuant to Rule 433

File No. 333-256167

July 12, 2021

Free Writing Prospectus

Pyxis Tankers Inc. Investor Presentation

This free writing prospectus relates to the public offering (the “Offering”) of 7.75% Series A Cumulative Convertible Preferred Shares of Pyxis Tankers Inc. (the “Company”), which are being registered pursuant to a Registration Statement on Form F-3 (File No. 333-256167) (the “Registration Statement”). This free writing prospectus should be read together with the preliminary prospectus dated July 12, 2021 included in the Registration Statement, relating to the Offering, including the documents incorporated by reference therein, which can be accessed through the following link: https://www.sec.gov/Archives/edgar/data/1640043/000149315221016595/form424b5.htm

Before you invest, you should read the preliminary prospectus in the Registration Statement and other documents the Company has filed with the U.S. Securities and Exchange Commission (the “SEC”) for more complete information about the Company and the Offering. You may obtain these documents for free by visiting EDGAR on the SEC website at www.sec.gov. Copies of the preliminary prospectus related to this Offering may be obtained from ThinkEquity, a division of Fordham Financial Management Inc., at 17 State Street, 22^nd Floor, New York, New York 10004, by calling (877) 436-3673 or by emailing prospectus@think-equity.com. The final terms of the Offering will be disclosed in a final prospectus to be filed with the SEC.