Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on FormS-3 of Spyre Therapeutics, Inc.of our report dated February 29, 2024 relating to the financial statements, which appears in Spyre Therapeutics, Inc.'s Annual Report on Form 10-K for the year ended December 31, 2023. We also consent to the reference to us under the heading“Experts”in such Registration Statement.

/s/ PricewaterhouseCoopers LLP

Austin, Texas

September 6, 2024

1

PricewaterhouseCoopers LLP, 835 W. 6th Street, Suite 1600, Austin, TX 78703

T: (512) 477 1300, www.pwc.com/us