Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the following Registration Statements:

1. Registration Statement (Form S-8, No. 333-221306) pertaining to the 2011 Stock Incentive Plan, the 2017 Stock Option and Incentive Plan, and the 2017 Employee Stock Purchase Plan of Allena Pharmaceuticals, Inc.,

2. Registration Statement (Form S-8, No. 333-223939) pertaining to the 2017 Stock Option and Incentive Plan and the 2017 Employee Stock Purchase Plan of Allena Pharmaceuticals, Inc.,

3. Registration Statements (Form S-3, Nos. 333-228656 and 333-250992) of Allena Pharmaceuticals, Inc., and

4. Registration Statements (Form S-8, Nos. 333-230127 and 333-237218) pertaining to the 2017 Stock Option and Incentive Plan of Allena Pharmaceuticals, Inc.;

of our report dated March 11, 2021, with respect to the consolidated financial statements of Allena Pharmaceuticals, Inc., included in this Annual Report (Form 10-K) for the year ended December 31, 2020.

/s/ Ernst & Young LLP

Boston, Massachusetts

March 11, 2021