UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

WASHINGTON, D.C. 20549

SCHEDULE 14A

(Rule 14a-101)

SCHEDULE 14A INFORMATION

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Securities Exchange Act of 1934

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☐ Definitive Proxy Statement

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☐ Soliciting Material Pursuant to §240.14a-12

STOKE THERAPEUTICS, INC.

(Name of Registrant as Specified In Its Charter)

N/A

(Name of Person(s) Filing Proxy Statement, if other than the Registrant)

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TICS INC Your Vote Counts!STOKE THERAPEUTICS, INC. 2024 Annual Meeting Vote by June 04, 2024 11:59 PM ETYou invested in STOKE THERAPEUTICS, INC. and it’s time to vote! You have the right to vote on proposals being presented at the Annual Meeting. This is an important notice regarding the availability of proxy material for the shareholder meeting to be held on June 05, 2024.Get informed before you vote View the Notice & Proxy Statement, Form 10-K online OR you can receive a free paper or email copy of the material(s) by requesting prior to May 22, 2024. If you would like to request a copy of the material(s) for this and/or future shareholder meetings, you may (1) visit www.ProxyVote.com, (2) call 1-800-579-1639 or (3) send an email to sendmaterial@proxyvote.com. If sending an email, pleaseinclude your control number (indicated below) in the subject line. Unless requested, you will not otherwise receive a paper or email . PV For complete information and to vote,visitwww.ProxyVote.comControl # Smartphone users Point your camera here and vote without entering a control number VoteVirtually at the Meeting June 05, 2024 9:00 AM EDT Virtually at: www.virtualshareholdenmeeting.com/STOK2024 *Please check the meeting materials for any special requirements for meeting attendance.

Vote at www.ProxyVote.com THIS IS NOT A VOTABLE BALLOT This is an overview of the proposals being presented at the upcoming shareholder meeting. Please follow the instructions on the reverse side to vote these important matters. Board Recommends. Voting Items 1. Election of Class II Directors to serve until the 2027 annual meeting of shareholders or until their successor has been duly elected and qualified. For Nominees: 01) Jennifer C. Burstein 02) Arthur A. Levin 03) lan F. Smith 2. To ratify the appointment of KPMG LLP as our independent registered public accounting firm for the fiscal year endingDecember31,2024. For NOTE: Such other business as may properly come before the meeting or any adjournment thereof. Prefer to receive an email instead? While voting on www.ProxyVote.com, be sure to click “Sign up for E-delivery”. Vote at www.ProxyVote.com THIS IS NOT A VOTABLE BALLOT This is an overview of the proposals being presented at the upcoming shareholder meeting. Please follow the instructions on the reverse side to vote these important matters. Board Recommends.VotingItems 1. Election of Class II Directors to serve until the 2027 annual meeting of shareholders or until their successor has been duly elected and qualified. For Nominees: 01) Jennifer C. Burstein 02) Arthur A. Levin 03) lan F. Smith 2. To ratify the appointment of KPMG LLP as our independent registered public accounting firm for the fiscal year ending December 31, 2024. For NOTE: Such other business as may properly come before the meeting or any adjournment thereof. Prefer to receive an email instead? While voting on www.ProxyVote.com, be sure to click “Sign up for E-delivery”.