| Corporate Presentation October 2022 |
| 2 Safe Harbor Statement This presentation includes forward - looking statements about, among other things, Verastem Oncology’s programs and product candidates, including anticipated regulatory submissions, approvals, performance and potential benefits of Verastem Oncology’s product candidates, that are subject to substantial risks and uncertainties that could cause actual results to differ materially from those expressed or implied by such statements. Applicable risks and uncertainties include the risks and uncertainties, among other things, regarding: the success in the development and potential commercialization of our product candidates, including defactinib and other compounds in combination with VS - 6766; the occurrence of adverse safety events and/or unexpected concerns that may arise from additional data or analysis or result in unmanageable safety profiles as compared to their levels of efficacy; or our ability to obtain, maintain and enforce patent and other intellectual property protection for our product candidates. Other risks and uncertainties include those identified under the heading “Risk Factors” in the Company’s Annual Report on Form 10 - K for the year ended December 31, 2021, as filed with the Securities and Exchange Commission (SEC) on March 28, 2022, and in any subsequent filings with the SEC, which are available at www.sec.gov and www.verastem.com. The forward - looking statements in this presentation speak only as of the original date of this presentation, and we undertake no obligation to update or revise any of these statements. |
| 3 We are a biopharmaceutical company committed to developing and commercializing new medicines for patients battling cancer Verastem Oncology Well Positioned to Capitalize on Growth Opportunities VS - 6766 (RAF/MEK clamp) and defactinib (FAK inhibitor) are clinically active against RAS mutant cancers 30% of all human cancers are driven by mutations in RAS ; VS - 6766 combinations potentially broadly applicable across a variety of tumor types. Clinical collaborations with Amgen & Mirati evaluating the combinations of VS - 6766 with sotorasib & adagrasib, respectively, in KRAS G12C mutant NSCLC supported by strong pre - clinical rationale Multiple clinical opportunities across RAS pathway - driven cancers based on preclinical data Up to $150 million of non - dilutive funding available from new credit facility Cash balance of $94.3 million as of June 30, 2022 & $110.4 million as of August 31, 2022 Company ended Quarter 2 2022 with $19.6 million non - GAAP operating expenses Lead clinical program has best - in - class potential Patent Update Strong balance sheet Rapid development path to market FDA Breakthrough Therapy Designation in LGSOC; Registration - directed trial initiated in 4Q 2020 in low - grade serous ovarian cancer (LGSOC) * Q2 2022 GAAP operating expenses - $21.4M minus Q2 2022 stock compensation - $1.8M = $19.6M Q2 2022 non - GAAP operating expenses Recently issued intermittent dosing IP for both VS - 6766 alone and VS - 6766 + defactinib extends patent coverage up to 2038 and 2040 Significant downstream market opportunity and blockbuster potential |
| 4 Key VSTM Milestones 2021 - 2022 LGSOC NSCLC 1H2021 2H2021 1H2022 RAMP 201 Amended to Include KRAS wt patients in Selection Phase RAMP 201 Target enrollment of Selection Phase Complete Initiated enrollment of Expansion Phase RAMP 202 Complete enrollment of Selection Phase Updated data from FRAME NSCLC cohort Presented at AACR Updated data from FRAME LGSOC cohort Presenting at ESMO FDA Breakthrough Therapy Designation VS - 6766 + Sotorasib Collaboration w/Amgen VS - 6766 + Adagrasib Collaboration w/Mirati 4Q2022 Additional Indications* Initiate combo study of VS - 6766 + cetuximab in KRAS mt CRC RAMP 201 Selection Phase Update* RAMP 201 Complete enrollment of Expansion Phase Translational data from FRAME LGSOC cohort presented at AACR Initiate RAMP 204 (VS - 6766 + adagrasib) in KRAS G12C (Mirati) Initiate RAMP 203 (VS - 6766 + sotorasib) in KRAS G12C (Amgen) Top - Line Data from RAMP 202 Selection Phase Top - Line Data from VS - 6766 + everolimus in KRAS mt Initiate combo study of VS - 6766 + abemaciclib and fulvestrant in ER+ breast cancer Initial readout of RAMP 203 data *Next RAMP 201 update expected to be provided once go - forward treatment regimen determined, timing of which will be driven by da ta maturity **Investigator - sponsored research Initiate basket trial of VS - 6766 + defactinib in RAS pathway - driven gynecological cancers ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ RAMP 202 Amended to include BRAF mt cohorts ✓ ✓ PanCAN Agreement Executed ✓ RAMP 201 FDA Meeting ✓ ✓ Initiate combo study of VS - 6766 + gemcitabine/nab - paclitaxel + defactinib in metastatic pancreatic cancer RAMP 201 Second Interim Update ✓ ✓ |
| 5 VS - 6766 is a Differentiated, Potentially Best - in - Class Asset Applicable Across Multiple Patient Populations • Unique RAF/MEK clamp mechanism of action • Novel intermittent dosing schedule; convenient oral regimen • Breakthrough Therapy Designation in recurrent low - grade serous ovarian cancer • Potential best - in - class safety & tolerability profile relative to marketed MEK inhibitors, with potential for combinability with agents from multiple target classes • Promising signals of clinical activity in various RAS - driven cancers, including in patients whose tumors previously progressed on other MEK inhibitors • Preclinical anti - proliferative activity across multiple MAPK pathway alterations (e.g. KRAS, NRAS, BRAF, NF1 mt) and multiple solid tumor indications • Strong preclinical combination data with other agents targeting RAS pathway and parallel pathways |
| 6 INDICATION REGIMEN STUDY NAME PRECLINICAL PHASE 1 PHASE 2 PHASE 3 CLINICAL COLLABORATION WITH LGSOC 1,2 VS - 6766 +/ - defactinib RAMP 201 R/R LGSOC VS - 6766 + defactinib FRAME R/ R endometrioid cancer (RAS/RAF mt) VS - 6766 + defactinib FRAME Gynecological Cancers (RAS Pathway - driven) 4 VS - 6766 + defactinib IST Mesonephric 4 VS - 6766 + defactinib IST R/R NSCLC (BRAF mt) VS - 6766 + defactinib RAMP 202 R/R NSCLC (KRAS G12C mt) VS - 6766 + sotorasib RAMP 203 R/R NSCLC (KRAS G12C mt) 3 VS - 6766 + adagrasib RAMP 204 Pancreatic Ductal Adenocarcinoma VS - 6766 + gemcitabine/nab - paclitaxel + defactinib RAMP 205 R/R NSCLC (KRAS mt) VS - 6766 + everolimus (mTORi) IST R/R Colorectal Cancer VS - 6766 + cetuximab (EGFRi) IST ER+ Breast Cancer VS - 6766 + abemaciclib + fulvestrant IST Robust Clinical Program: VS - 6766 in multiple combinations across RAS/MAPK pathway - driven tumors 1 FDA Breakthrough Therapy Designation 2 Registration - directed trial 3 In Startup 4 Preclinical studies underway, ph. 2 investigator - sponsored trials in preparation |
| 7 Key Financial Statistics Cash, cash equivalents & investments $94.3M ($110.4M as of August 31, 2022) Non - GAAP Operating Expenses $19.6M Shares Outstanding 187.6M (210.0M as of August 31, 2022) As of and for the quarter ended June 30, 2022 Oxford Finance LLC Credit Facility Loan Tranches Event A $25M At closing B $15M COPIKTRA PTCL approval in U.S. or $50M equity proceeds C $25M LGSOC accelerated or full approval D $35M $50M product revenue on six months trailing basis E $50M Lender discretion Total $150M Interest rate: floating rate, which is subject to a floor and a cap; 5% final payment charge, and loan subject to 1 - 3% early payment fee Term: 5 Years; Interest only two years initially, extendable up to four years based on achievement of milestones Financial covenants: None * Q2 2022 GAAP operating expenses - $21.4M minus Q2 2022 stock compensation - $1.8M = $19.6M Q2 2022 non - GAAP operating expenses |
| VS - 6766 RAF/MEK Clamp Program Overview |
| 9 Broad Development Opportunities Across Multiple RAS/MAPK Pathway - Driven Cancers VS - 6766 High Priority Registration Indication Registration - Directed Trial Initiated in 4Q20 LGSOC 1,2 (RAMP 201) Key Signal Finding VS - 6766 + G12Ci KRAS G12C mt NSCLC 2 (RAMP 203 - sotorasib) & (RAMP 204 - adagrasib) BRAF mt (V600E & non V600E) NSCLC 1,2 (RAMP 202) Pancreatic 2 RAS/RAF mt endometrioid 1 Uveal Melanoma 2 VS - 6766 + everolimus KRAS mt NSCLC 1,2 VS - 6766 + cetuximab KRAS mt CRC 2 VS - 6766 + abemaciclib and fulvestrant in ER+ breast cancer Rational Combinations G12Ci 1,2 G12Di 2 Anti - EGFR 2 Everolimus 1,2 CDK4/6 inhibitor 2 Anti - PD - 1 1,2 Chemotherapy 2 RAS Pathway Dependent Cancers Gynecological 1,2 NSCLC 1,2 Colorectal 1,2 Melanoma 1,2 Pancreatic 2 Thyroid 1,2 Biomarker Selection KRAS mt 1,2 BRAF mt (V600 & non - V600) 1,2 NRAS mt 1,2 CRAF mt/fusions 2 1 Supported by clinical data 2 Supported by preclinical data |
| 10 VS - 6766 is a Unique Small Molecule RAF/MEK Clamp VS - 6766 (RAF/MEKi) Mirdametinib (MEKi) VS - 6766 inhibits MEK, BRAF & CRAF by trapping these molecules in inactive complexes MEK inhibitors paradoxically induce MEK phosphorylation (pMEK) by relieving ERK - dependent feedback inhibition of RAF By inhibiting RAF phosphorylation of MEK, VS - 6766 has advantage of not inducing pMEK VS - 6766 inhibits ERK signaling more completely; may confer enhanced therapeutic activity References : Ishii et al., Cancer Res , 2013; Lito et al., Cancer Cell , 2014 |
| 11 VS - 6766 is a Unique RAF/MEK Clamp which Induces Inactive Complexes of MEK with ARAF, BRAF & CRAF Contrasting mechanism of action vs. trametinib Deborah Morrison unpublished RTK RAS RAF MEK ERK Tumor Growth Growth factors + VS - 6766 Improved ERK blockade Feedback reactivation is blocked; Sustained inhibition Formation of inactive RAF/MEK complex to block ARAF, BRAF & CRAF signaling DMSO Tram VS - 6766 DMSO Tram VS - 6766 RTK RAS RAF MEK ERK Tumor Growth KSR Growth factors + Trametinib Feedback reactivation of RAF maintains pMEK; Adaptive resistance |
| 12 VS - 6766 Inhibits Cell Proliferation Across Multiple RAS/MAPK Pathway Alterations and Multiple Solid Tumor Histologies Reference: Adapted from Pachter RAS - Targeted Drug Discovery , Sep 2021 3D proliferation assay SW1463 MIAPACA2 H358 H1373 SW837 H2122 H2030 SW1573 HPAC ASPC1 HPAFII SNU-C2B LS513 LS 180 SKLU1 A427 SNU-C2A H441 SW403 SK-CO-1 SW620 PANC0327 H2291 SNG-M SW480 H2444 CAPAN2 CFPAC1 PSN1 KP2 HUP-T3 H2009 H1573 SW1116 A549 LS123 NCI-H747 HCT 116 DLD-1 T84 HCT-15 Calu-6 HT-29 WM-266-4 SW1417 A-375 C32 SK-MEL-5 IGR-1 Colo-205 A2058 HS852.T RKO NCI-H2087 RL95-2 NCI-H2087 GAK SK-MEL-2 HEC-151 H1573 DLD-1 HS940.T H1373 HMCB HCT 116 RKO SW48 AN3 CA RL95-2 LS513 5637 0.01 0.1 1 10 VS-6766 IC50 (3D proliferation assay) VS-6766 IC50 ( KRAS G12V KRAS G12D BRAF V600E KRAS G12C Indication NSCLC Panc CRC Indication: MAPK pathway lateration: Melanoma NF1 mt NRAS mt KRAS G12R BRAF class 2 mt KRAS G12C KRAS G12D KRAS G12V Other KRAS mt BRAF mt NRAS mt ARAF mt RAF1 mt Endometrial Bladder NF1 mt KRAS G12S KRAS G13D KRAS Q61K KRAS G12A ERK2 mt Other mt |
| 13 Vertical Blockade: Establishing VS - 6766 as the Backbone of Therapy for RAS/MAPK Pathway - Driven Tumors ▪ Current Challenges • Blocking any single target in the pathway is insufficient for maximum depth and duration of anti - tumor efficacy • e.g., SHP2i, KRAS - G12Ci, RAFi, MEKi, ERKi • Vertical blockade concept is now well established • Necessary to block more than 1 target in the pathway • Many of these agents (e.g., SHP2i, MEKi) have poor tolerability as monotherapy and in combination ▪ Solutions offered by VS - 6766 • Vertical blockade (RAF and MEK blockade) in a single drug • Potential best - in - class tolerability with recommended twice weekly dosing regimen • Should enable tolerable combinations • Compelling synergy data (preclinical) for VS - 6766 combinations (e.g., with KRAS - G12C inhibitors) supporting clinical combinations RTK Growth factors EGFRi FGFRi G12Ci RAFi MEKi ERKi VS - 6766 SHP2i SOS1i RAS RAF MEK ERK Tumor Growth References: 1 Chen, Mol Cancer Res 2018; 2 Banerji, BTOG Dublin, Jan 23, 2019 |
| 14 Parallel Pathway Inhibition: Establishing VS - 6766 as the Backbone of Therapy for RAS/MAPK Pathway - Driven Tumors ▪ Current Challenges • Blocking Ras pathway can be circumvented through parallel pathways • e.g., PI3K/AKT/mTOR, FAK, RhoA, YAP • Combinations of MEKi + AKTi have shown poor tolerability ▪ Solutions offered with VS - 6766 • Good tolerability with twice weekly VS - 6766 opens up intermittent dosing options for combinations • Compelling preclinical synergy data with VS - 6766 in combination with several key anti - cancer agents • RP2D established for VS - 6766 + defactinib and for VS - 6766 + mTORi (everolimus) with twice weekly regimen β α Integrin FAK Extracellular Matrix P PI3K AKTi mTORi FAKi SRC AKT RTK Growth factors G12Ci RAFi MEKi ERKi VS - 6766 RAS RAF MEK ERK SHP2i SOS1i Tumor Growth EGFRi FGFRi RhoA, YAP, etc. mTOR References: 1 Chen, Mol Cancer Res 2018; 2 Banerji, BTOG Dublin, Jan 23, 2019 |
| 15 Favorable Tolerability Profile with Novel Intermittent Dosing Regimen VS - 6766 monotherapy Daily at MTD N=6 28 - day cycle RP2D VS - 6766 monotherapy 4mg twice weekly N=26 28 - day cycle RP2D (VS - 6766 3.2mg twice weekly + defactinib 200mg twice daily) N=38 21 days of 28 - day cycle Treatment Related Adverse Event Grade ≥3 Grade ≥3 Grade ≥3 Rash 3 (50%) 5 (19%) 2 (5%) CK elevation (Creatine phosphokinase) 1 (17%) 2 (8%) 2 (5%) 1 Chenard - Poirier, et al .. ASCO 2017 References: Banerji, Q4 2020 report; Data on file RP2D: recommended phase 2 dosing Summary of Adverse Events Grade ≥ 3 Occurring in ≥ 5% of patients Summary of FRAME Safety Profile Most Adverse Events (AE) were Grade 1/2 Few patients have discontinued due to AEs in the study |
| 16 Favorable Tolerability Profile at Recommended Phase 2 Dose for VS - 6766 plus Defactinib Combination Regimen Treatment Related Adverse Events Details* (≥10% patients in cohort 3.2mg 6766 and Def 200mg ) VS - 6766 4mg Twice Weekly (4 wks of every 4 wks) 1 n=22 VS - 6766 3.2mg Twice Weekly Def 200mg BID (3 wks of every 4 wks) 2 n=38 Gr1/2 Gr3/4 Gr1/2 Gr3/4 Rash 15 5 32 2 CK Elevation 13 2 19 2 AST Elevation 1 13 Hyperbilirubinemia 14 1 Visual Disturbance 13 9 ALT Elevation 2 5 Diarrhoea 6 1 14 1 Fatigue 5 1 8 1 Oral Mucositis^ 7 1 11 Nausea 5 5 Vomiting 2 4 Peripheral Edema 9 10 Paronychia 3 4 Thrombocytopenia 6 Pruritus 3 0 5 Summary of FRAME Safety Profile Most Adverse Events (AE) were Grade 1/2 Few patients have discontinued due to AEs in the study RP2D VS - 6766 3.2 mg oral twice wkly (3 wks of every 4 wks) Defactinib 200 mg oral BID (3 wks of every 4 wks) *AEs were graded by NCI CTC v4; highest grade only recorded for each patient; AEs presented in ≥10% Patient (cohort 3.2mg 6766 and Def 200mg) data preliminary and subject to change; ^also includes glossitis/mouth ulcers References: 1 Data on file VS - 6766 Investigator’s Brochure; 2 Banerji, Q4 2020 report |
| VS - 6766 +/ - Defactinib in Low - Grade Serous Ovarian Cancer |
| 18 70% of LGSOC Tumors Driven by Mutations in the RAS/MAPK Pathway LGSOC is a type of ovarian cancer that disproportionately affects younger women Patients often experience significant pain and suffering from their disease over time A slow growing cancer, that has a median survival of almost 10 years, so patients remain in treatment for a long time (10 - yr prevalence ~80,000 worldwide, ~6,000 US) Most prior research has focused on high grade serous ovarian cancer (HGSOC). However, LGSOC is clinically, histologically and molecularly unique from HGSOC with limited treatments available 1,000 to 2,000 patients in the U.S. and 15,000 to 30,000 worldwide diagnosed with LGSOC each year ~30% of LGSOC Patients Have KRAS mt ~70% of LGSOC Shows RAS Pathway - Associated mts References: AACR Project GENIE Cohort v9.0 - public and Verastem unpublished analysis Reference: Monk, Randall, Grisham, The Evolving Landscape of Chemotherapy in Newly Diagnosed Advanced Epithelial Ovarian Canc er, Am Soc Clin Oncol Educ Book; 2019; Slomovitz, Gourley, Carey, Malpica, Shih, Huntsman, Fader., Grisham et al, Low - Grade serous ovarian cancer: State of the Science ; Gynecol Oncol; 2020. Grisham, Iyer, Low - Grade Serous Ovarian Cancer: Current Treatment Paradigms and Future Directions; Curr Treat Options Oncology; 2018. KRAS mutant , 30% NRAS, BRAF, ARAF mutant , 20% Other RAS - associated gene mutations , 20% Non - RAS - associated , 30% |
| 19 LGSOC: Limited Treatment Options with High Unmet Need References: 1 NCCN guidelines v1.2022 2 No standard sequencing of drugs for recurrence disease Low - Grade Ovarian Cancer – Treatment Algorithm 1 Observe only Carbo - Pt + Paclitaxel + hormonal (2b) OR Chemo + Beva for Stage II - IV (2a) OR Hormonal Tx (2b) Clinical trial (2a) Chemotherapy (Pt - based combination or monotherapy) (2a) Hormonal Tx (2a) Trametinib (2a) Binimetinib (2b) Recurrence Therapy 2 Stage IC Stage II - IV Stage IA - IB Therapy Response Rate ORR Median PFS Months (95% CI) Discontinuation Rate due to AEs Standard of Care 1 6% 7.2 (5.6 - 9.9) 12 % Trametinib 1 26%* 13.0 (9.9 - 15.0) 35% Standard of Care 2 13% 10.6 (9.2 to 14.5) 17% Binimetinib 2 16% 9.1 (7.3 - 11.3) 31% 1 Study GOG 281 trial Gershenson et al., Lancet 2022 2 MILO Study Monk et al., J Clin Oncol 2020. Standard of Care = letrozole, tamoxifen, chemotherapy PFS = Progression free survival CI = confidence interval * Not confirmed by central review Recent Clinical Trials in Recurrent LGSOC |
| 20 VS - 6766 in Combination with Defactinib (FRAME) Shows Promising ORR with Durability in Refractory LGSOC with Expanded Number of Patients (n=24) Reference: Banerjee et al., ESMO Sept 2021 Data cut off April 2021 PFS: Progression free survival NR: Not reached • Overall response rate (ORR) = 46% (11 confirmed PRs/24) o KRAS mutant ORR = 64% (7 confirmed PRs/11) o KRAS wild - type ORR = 44% (4 confirmed PRs/9) o KRAS status undetermined (1 unconfirmed PR/4) • Response too early to determine for 2 pts on study for 5 months • Responses in patients previously treated with MEKi • 54% (13/24) patients still on treatment • 1 patient discontinuing for adverse events as of April 2021 • Median PFS 23 months (95% CI 10.6 - NR) across all LGSOC 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 -70 -60 -50 -40 -30 -20 -10 0 10 20 Response by RECIST Time (cycles) Response (% change from baseline) FRA101001 - KRAS G12V FRA101002 - KRAS G12A FRA101009 - KRAS G12D FRA101012 - KRAS WT FRA101007 - KRAS WT FRA101014 - KRAS G12D FRA101015 - KRAS WT FRA101019 - KRAS G12D FRA101024 - KRAS WT FRA101025 - KRAS WT FRA102010 - KRAS WT FRA101028 - undocumented FRA101032 - KRAS D33E, I24N FRA101033 - KRAS G12D FRA101035 - KRAS G12D FRA101037 - KRAS WT FRA101038 - KRAS WT Continuing on treatment FRA101039 - KRAS WT FRA103001 - KRAS G12V FRA104001- KRAS D57-T58ins FRA103002 - undocumented FRA101042 - KRAS G12D FRA103003 - KRAS WT FRA102018 - KRAS WT FRA101007 FRA101014 FRA101042 FRA101012 FRA103003 FRA101032 FRA103002 FRA101038 FRA102018 FRA101015 FRA102010 FRA101019 FRA101024 FRA101028 FRA101039 FRA101025 FRA101037 FRA103001 FRA101035 FRA101033 FRA104001 FRA101009 FRA101001 FRA101002 -80 -70 -60 -50 -40 -30 -20 -10 0 10 Best Response (% change from baseline) Undocumented KRAS wt KRAS wt KRAS G12D KRAS wt KRAS D33E, I24N Undocumented Prior MEK inhibitor * * KRAS G12A KRAS G12V KRAS G12D KRAS G12V * KRAS wt KRAS G12D Confirmed partial response Unconfirmed partial response KRAS wt KRAS wt * * Best response by RECIST KRAS wt KRAS G12D KRAS wt KRAS wt KRAS G12D KRAS D57-T58ins * * * * * * Still on treatment * KRAS G12D Undocumented * * Undocumented Stable disease FRA101007 FRA101014 FRA101042 FRA101012 FRA103003 FRA101032 FRA103002 FRA101038 FRA102018 FRA101015 FRA102010 FRA101019 FRA101024 FRA101028 FRA101039 FRA101025 FRA101037 FRA103001 FRA101035 FRA101033 FRA104001 FRA101009 FRA101001 FRA101002 -80 -70 -60 -50 -40 -30 -20 -10 0 10 Best Response (% change from baseline) Undocumented KRAS wt KRAS wt KRAS G12D KRAS wt KRAS D33E, I24N Undocumented Prior MEK inhibitor * * KRAS G12A KRAS G12V KRAS G12D KRAS G12V * KRAS wt KRAS G12D Confirmed partial response Unconfirmed partial response KRAS wt KRAS wt * * Best response by RECIST KRAS wt KRAS G12D KRAS wt KRAS wt KRAS G12D KRAS D57-T58ins * * * * * * Still on treatment * KRAS G12D Undocumented * * Undocumented Stable disease |
| 21 RAMP 201 Registration - directed Phase 2 Trial of VS - 6766 +/ - Defactinib in Recurrent LGSOC - KRAS Mutant (mt) and Wild Type ( wt ) *Dosing: Defactinib + VS - 6766 combo: Defactinib 200mg PO BID: 21/28 days + VS - 6766 3.2mg PO 2x/wk 21/28 days; VS - 6766 monothera py: VS6766 4.0 mg PO 2x/wk 21/28 days **Expansion Phase – final sample size to be adjusted based on adaptive design Defactinib + VS - 6766 KRAS - mt (n=16) VS - 6766 Mono KRAS - mt (n=16) E ach cohort expanded to ~36 patients (~20 additional patients)** Go - forward treatment regimen to be selected once data sufficiently mature ** Selection Phase* Expansion Phase** Defactinib + VS - 6766 KRAS - wt (n=16) VS - 6766 Mono KRAS - wt (n=16) Now Enrolling E xpansion P hase Primary Endpoint: Objective Response Rate (blinded independent review) Evaluation of: 1) In KRAS mt patients 2) All patients (KRAS mt & wt) Recurrent LGSOC Prior chemotherapy Measurable disease (RECIST 1.1) Prior MEKi allowed Completed Enrollment Defactinib + VS - 6766 KRAS - mt (n=36) VS - 6766 Mono KRAS - mt (n=36) Defactinib + VS - 6766 KRAS - wt (n=36) VS - 6766 Mono KRAS - wt (n=36) |
| 22 RAMP 201 Update - October 2022 • Full enrollment based on the study protocol on track and expected by the end of the year • FDA meeting 4Q - 22 to align on regulatory path forward and go forward regimen • Completed second planned interim analysis • Encouraging efficacy results include independently confirmed responses • Enrollment continues towards completion of all four cohorts • No new safety signals, continued favorable safety profile • Majority of patients remain on treatment Next Steps Update |
| 23 LGSOC Market Opportunity Larger or Comparable to Other High Unmet Need KRAS Opportunities NSCLC KRAS G12C 3 Pancreatic 3 LGSOC 3 Endometrioid 3 Metastatic uveal melanoma 3 ~6K patients US 1 ~80K patients WW 1 Patient - months of Therapy Per Year 2 (across all 2L+ patients) 1 References : Monk, Randall, Grisham, The Evolving Landscape of Chemotherapy in Newly Diagnosed Advanced Epithelial Ovarian Cancer, Am Soc Cli n Oncol Educ Book; 2019; Slomovitz, Gourley, Carey, Malpica, Shih, Huntsman, Fader., Grisham et al, Low - Grade serous ovarian cancer: State of the S cience; Gynecol Oncol; 2020. Grisham, Iyer, Low - Grade Serous Ovarian Cancer: Current Treatment Paradigms and Future Directions; Curr Treat Options Oncology; 2018; Glo bocan 2020 2 Patient - months of Therapy metric calculated by multiplying relevant incidence/prevalence rate times estimated duration of thera py; represents US market opportunity only; patient population estimates from Globocan 2020, American Cancer Society 2021, AACR Genie Cohort V9.0 public, and scientific pub lications. Duration of therapy estimates from clinical studies and clinician experience. Patient - months on therapy is for 2 nd - line+ patients 3 NSCLC KRAS G12C 2 nd line patients (incidence); Pancreatic RAS/RAF mutant 2 nd - line patients (incidence); LGSOC KRAS mutant and wild - type patients (prevalence); Endometrioid RAS/RAF mutant 2 nd - line patients (incidence); Uveal melanoma RAS/RAF mutant 2nd - line patients (incidence) Prevalence - 50,000 100,000 150,000 ~4k ~2k wild type KRAS mutation |
| VS - 6766 +/ - Defactinib in NSCLC |
| 25 High Unmet Need in Refractory mt NSCLC Adenocarcinoma Advanced or Metastatic NSCL Cancer Recommend Histologic and Molecular Subtyping 5 Appropriate targeted agent Chemotherapy Docetaxel Gemcitabine Pemetrexed Prior PD - (L)1 No Prior PD - (L)1 PD - (L)1 Chemo PD - (L)1 PD - (L)1 single agent or PD - (L)1 + chemo Chemotherapy or clinical trials EGFR/ALK/ROS1/BRAF/ KRAS - G12C (targeted) Non - targeted PD - (L)1 1% Non - Targeted PD - (L)1 1% ▪ SOC in recurrent disease is chemotherapy ▪ Pre - PD - (L)1 era, chemotherapy response rate ~10% in recurrent disease; 12w PFS of 30 – 45% Recurrence Recurrence Verastem Clinical Trials: • RAMP 202: • BRAF V600E and BRAF non - V600E — VS - 6766 + Defactinib • RAMP 203 — KRAS G12C: VS - 6766 + sotorasib • RAMP 204 — KRAS G12C: VS - 6766 + adagrasib NSCLC Adenocarcinoma 3 0 5 10 15 % of Patients KRAS Mutation BRAF Mutation US Annual Incidence 1,2 : 92K WW Annual Incidence 1,2 : 836K References: 1 Globocan, 2018 2 https://www.ncbi.nlm.nih.gov/books/NBK519578/ 3 TCGA PanCancer Atlas (cBioPortal analysis) 4 www.thelancet.com Vol 389 January 21, 2017 5 Adapted from NCCN Non - small cell lung cancer guidelines Version 3.2020 6 Clinical Cancer Research DOI 10.1158/1078 - 0432.CCR - 18 - 2062 KRAS Mutations Represent 25% of Lung Cancer Adenocarcinoma & BRAF Represent 2 - 4% (EGFR 17%, ALK 7%) 4,6 |
| 26 RAMP 202: Phase 2 Trial of VS - 6766 +/ - Defactinib in Advanced NSCLC Primary Cohort: KRAS G12V mt NSCLC References: 1 Defactinib 200 mg PO BID (21/28 days) + VS - 6766 3.2 mg PO 2x/wk (21/28 days) 2 VS - 6766 4.0 mg PO 2x/wk (21/28 days) Advanced NSCLC 1 - 2 prior regimens 1 prior platinum - containing chemo; Prior CPI unless contraindicated Measurable disease (RECIST 1.1) Appropriate approved therapy for other relevant mutations No prior MEKi, no prior KRAS - specific targeted therapy No untreated CNS metastases ECOG OS 0 - 1 Defactinib + VS - 6766 1 KRAS mt G12V N=16 VS - 6766 2 KRAS mt G12V N=16 Criteria to continue to expansion phase not met Selection Phase Expansion Phase Completed Enrollment Defactinib + VS - 6766 1 KRAS mt non - G12V N=40, maximum Criteria to continue to expansion phase not met Completed Enrollment |
| 27 RAMP 202 Results – August 2022 • Results of Part A of RAMP 202 trial in KRAS - G12V mt NSCLC show VS - 6766 ± defactinib did not meet criteria to continue to expansion phase • Continue to analyze the results of the trial and integrate the findings into our development plans moving forward • Continuing VS - 6766 development in NSCLC with other combinations: o Sotorasib o Adagrasib o Everolimus • The confirmed ORR was 11% (2/19) in KRAS G12V mt NSCLC patients treated with VS - 6766 + defactinib with a disease control rate of 37% • The ORR in non - G12V KRAS mt patients treated with VS - 6766 + defactinib was 5% (2/37) with a disease control rate of 54% o No subtype was identified for further clinical evaluation of VS - 6766 + defactinib in this trial Next Steps Findings |
| 28 RAMP 202: VS - 6766 + Defactinib in BRAF mt NSCLC References: 1 Defactinib 200 mg PO BID (21/28 days) + VS - 6766 3.2 mg PO 2x/wk (21/28 days) Advanced NSCLC 1 - 2 prior regimens 1 prior platinum - containing chemo; Prior CPI unless contraindicated Measurable disease (RECIST 1.1) Appropriate approved therapy for other relevant mutations No prior KRAS - specific targeted therapy No prior MEKi, (except for BRAF mtV600E) No untreated CNS metastases ECOG OS 0 - 1 Defactinib + VS - 6766 1 BRAF mt V600E N=15 Defactinib + VS - 6766 1 BRAF mt non - V600E N=15 BRAF Mutant Mutation - specific cohort analyses for ORR BRAF Cohorts Expansion TBD based on results of analysis Initial Phase Analysis |
| 29 Preclinical Synergy of VS - 6766 + G12C Inhibitors in KRAS G12C mt Models Synergy of VS - 6766 + G12C inhibitors across G12C mutant NSCLC, CRC & Pancreatic cancer cell lines Doses Tested Trametinib: 0.3 mg/kg QD VS - 6766: 0.3 mg/kg QD FAKi: 50 mg/kg BID Sotorasib: 30 mg/kg QD Response @ Day 10 VS - 6766 & FAKi potentiate sotorasib efficacy in KRAS G12C mutant NSCLC in vivo; Tumor regression in all mice with triple combination VS - 6766 + sotorasib yields deeper and more sustained inhibition of ERK signaling pathway H2122 KRAS G12C mutant NSCLC Sotorasib VS - 6766 4h 48h p - ERK Actin Total ERK ND: not determined - - + - - + + + - - + - - + + + H2122 KRAS G12C mutant NSCLC Concentrations Tested Sotorasib: 100 nM VS - 6766: 100 nM Reference: Coma et al., AACR 2021 Combined Synergy Score Cell line Indication Sensitivity to G12C inhibitors VS - 6766 + sotorasib VS - 6766 + adagrasib H2122 NSCLC Moderately sensitive 44.7 44.6 H1373 NSCLC Sensitive 10.0 3.4 SW1573 NSCLC Insensitive 8.6 12.0 H358 NSCLC Sensitive 6.9 5.4 H2030 NSCLC Moderately sensitive 5.1 ND SW837 CRC Sensitive 16.1 18.5 MIAPACA2 Panc Sensitive 2.3 5.3 |
| 30 Acquired Resistance Mechanisms to KRAS G12Ci Treatment in Patients Further Support Combination of KRAS G12Ci with VS - 6766 Summary of Putative Mechanisms of Acquired Resistance to Adagrasib Treatment • Mechanisms of acquired resistance to KRAS G12Ci adagrasib treatment in patients recently reported 1,2 • The main resistance alterations occurred in • RTK mts or amplifications • KRAS mts or amplification • NRAS mt • BRAF V600E mt, BRAF or CRAF fusions • MAP2K1 (MEK1) mt/deletion • VS - 6766 has shown activity against these KRAS, NRAS, BRAF and CRAF modifications Reference: Andrew Aguirre, unpublished Reference: 1 Awad MM et al., N Engl J Med 2021; 384: 2382 - 93; 2 Tanaka et al., Cancer Discov 2021;11:1 – 10 |
| 31 Patients must have known G12C KRAS mutation determined using validated test Treatment with at least 1 but no more than 3 prior systemic regimens, for Stage 3B - C or 4 NSCLC* Patient may have previously received adjuvant chemotherapy for earlier - stage disease Measurable disease according to RECIST 1.1 ECOG performance status ≤ 1 RAMP 203: Phase 1/2 Trial of VS - 6766 + LUMAKRAS TM (sotorasib) in KRAS G12C - mutated Advanced NSCLC RP2D Selection Part A: Dose Evaluation (3+3 DLT Assessment) Part B: Dose Expansion at RP2D (Primary endpoint ORR) Cohort 1 Patients without Prior KRAS G12C Inhibitor Treatment Stage 1 : ~20 patients Stage 2 : expand VS - 6766 + Sotorasib Dose Finding Cohorts (N= 3 - 6 pts)* Cohort 2 Patients who Progressed on KRAS G12C Inhibitor Treatment Stage 1 : ~20 patients Stage 2 : expand Collaboration with Amgen *may include patients with or without prior G12C therapy *H as advanced to Cohort 2: 4mg VS - 6766/960mg sotorasib |
| 32 RAMP 204: Phase 1/2 Trial of VS - 6766 + Adagrasib in KRAS G12C - mutated Advanced NSCLC Patients must have a KRAS G12C mutation determined using validated test Treatment with at least 1 but no more than 3 prior systemic regimens, for Stage 3B - C or 4 NSCLC Patient must have received prior therapy with a KRAS G12C inhibitor and experience progressive disease Measurable disease according to RECIST 1.1 ECOG performance status ≤ 1 Part A: Dose Evaluation (3+3 DLT Assessment) Part B: Dose Expansion (Primary endpoint ORR) Stage 1 : 19 patients (including Part A patients) treated with RP2D Stage 2 : expand to 55 patients RP2D Selection VS - 6766 + Adagrasib Dose Finding Cohorts (N= 3 - 6 pts) Collaboration with Mirati Therapeutics |
| Future Opportunities: VS - 6766 as Backbone of RAS Therapy |
| 34 High Unmet Needs in RAS/MAPK Pathway - Driven Cancers NSCLC Incidence 3,5 : 194K Colorectal Incidence 5 : 148K Pancreatic Incidence 5 : 58K Uterine Endometrioid Incidence 4,5 : 59K Melanoma Incidence 5 : 108K Multiple Myeloma Incidence 5 : 32K Melanoma Incidence 5 : 108K Colorectal Incidence 7 : 148K Papillary Thyroid Incidence 5,6 : 42K Ovarian Incidence 5 : 22K Incidence References: 1 Reference for RAS mt frequencies – Cox et al. Nature Reviews 13: 828, 2014; 2 Reference for BRAF mt frequencies – Turski et al. Mol Cancer Ther 15: 533, 2016 3 85% of lung cancer is NSCLC (Lu et. al. Cancer Manag Res. 2019) ; 4 90% of all uterine cancers are of the endometrial type (ACS) ; 5 Cancer Statistics 2020, Siegel et. al. CA Cancer J Clin 2020;70:7 - 30 ; 6 8 out of 10 thyroid cancers are of the papillary type (ACS) 7 CbioPortal References: McCormick F Clin Cancer Res 15April2015; 6 Adderley H et al. EBioMedicine 01Mar2019; Papke B et al. Science 17Mar2017; Ryan M et al. Nature Reviews Clinical Oncology 01Oct2018; NIH cancer.gov/research/key - initiatives/ras KRAS - mutant Cancers 1 31% 45% 98% 21% NRAS - mutant Cancers 1 28% BRAF - mutant Cancers 2 60% 10% 30 – 80% 20% 5% Challenges with conventional approaches Modest progress; limited number of approved therapies Single agent therapies (e.g., MEK inhibitors) associated with resistance Tolerable combination regimens with MEK inhibitors have been challenging Current RAS inhibitors in development address only a minority of all RAS mutated cancers Breadth of potential opportunity 30% of all human cancers are driven by mutations of the RAS family of genes 6 Established prognostic significance Patients with mutations of the RAS family have an overall worse prognosis NSCLC Incidence 3,7 : 194K 7% |
| 35 Preclinical Synergy of VS - 6766 in Combination with Promising Agents for Clinical Investigation H2122 SW837 H1373 SW1573 H358 H2030 MIAPACA2 0 10 20 30 40 50 VS-6766 + AMG 510 Combined Synergy Score NSCLC Panc Synergy Antagonism Indication CRC VS - 6766 + G12Ci (sotorasib) H2122 SW837 SW1573 H358 MIAPACA2 H1373 0 10 20 30 40 50 VS-6766 + MRTX849 Combined Synergy Score NSCLC PDAC Synergy Antagonism Indication CRC VS - 6766 + G12Ci (adagrasib) H2122 MIAPACA2 H1373 H358 SW1573 A427 HPAC ASPC1 HPAFII SKLU1 CFPAC1 CAPAN2 H2291 PANC0327 H441 H2444 -20 -10 0 10 20 30 40 VS-6766 + Palbociclib Combined Synergy Score KRAS G12C KRAS G12D KRAS G12V 100% (5/5) 50% (3/6) 100% (5/5) Indication NSCLC PDAC Synergy Antagonism VS - 6766 + mTORi (Everolimus) Vertical MAPK Pathway Inhibition Parallel Pathway Inhibition KP4 Panc 04.03 Panc 08.13 AsPC-1 HPAF-II HPAC LS 180 LS513 SNU-C2B SNU-C2A Gp2D A427 SK-LU-1 0 20 40 60 VS-6766 + MRTX1133 Combined Synergy Score Indication NSCLC CRC Synergy Antagonism Indication Panc VS - 6766 + SHP2i (RMC - 4550) VS - 6766 + SOS1i (BI - 3406) VS - 6766 + CDK4/6i (Palbociclib) VS - 6766 + pan - HERi (Afatinib) VS - 6766 + G12Di (MRTX1133) |
| 36 VS - 6766 + Everolimus Clinical Data Presented at ASCO • Well - tolerated RP2D established for VS - 6766 + everolimus with intermittent dosing of both agents (twice weekly; 3 wks on/1 wk off) o No DLTs reported at RP2D • VS - 6766 + everolimus combo induced PRs in patients with various RAS mutations in NSCLC, LGSOC and thyroid cancers • Both LGSOC pts showed PRs with 69% and 79% reduction and have been on treatment for ≥3 years with treatment ongoing • KRAS mutant NSCLC expansion cohort is currently ongoing – expanding to 20 pts o Currently 2 PRs/11 o Median progression free interval of 6.25 months in heavily pre - treated patients NRAS Q61K Anaplastic thyroid cancer (lung metastasis) VS - 6766 + Everolimus Pre - treatment KRAS G13A NSCLC KRAS G12D LGSOC (liver metastasis) Reference: Minchom et al., ASCO 2022 |
| 37 Combination of VS - 6766 with anti - EGFR mAb Induces Tumor Regression in a KRAS mt Colorectal PDX Model • VS - 6766 + anti - EGFR (panitumumab) induces tumor regression in a KRAS G12V mt CRC patient - derived xenograft model • G12Ci + anti - EGFR (sotorasib + panitumumab and adagrasib + cetuximab) have shown partial responses in KRAS G12C mt CRC (Fakih et al. ESMO 2021; Weiss et al. ESMO 2021) • These data support clinical testing of VS - 6766 + anti - EGFR (cetuximab) for treatment of KRAS mt CRC (NCT05200442) Vehicle VS - 6766 Panitumumab (anti - EGFR) Panitumumab + VS - 6766 Collaboration with Marwan Fakih, City of Hope Pachter, RAS Development Summit, 2021 |
| 38 VS - 6766 Patent Exclusivity Composition of Matter Method of Making Sept 2032 Dosing Protocol May 2038 Combination w/ Defactinib 2041 - 2042 if issued Feb 2027 + 5 yrs (PTE) = 2032 Sept 2040 Methods of Treating PTE 2041 - 2042 if issued Combinations |
| Backup Slides |
| 40 VS - 6766 Inhibits CRAF - The key driver of KRAS G12V mt NSCLC References: Ishii et al. Cancer Res (2013), Blasco, R. B. et al. Cancer Cell (2011), Lito, P. et al. Cancer Cell (2014), Sanclemente, M. et al. Cancer Cell (2018) A Precision Approach to KRAS G12V Driven NSCLC CRAF, but not BRAF, ablation improves survival of mice with KRAS G12V induced lung cancer in vivo KRAS G12V signals mainly through RAF/MEK in contrast to other variants, such as KRAS - G12D, which signal more through PI3K/AKT KRAS G12V models are especially dependent on CRAF KRAS G12V CRAF PI3K MEK/ERK AKT/mTOR Tumor Growth CRAF Drives KRAS G12V mt NSCLC 1 +83% OS CRAF KO Shows Strong Efficacy BRAF KO Has No Effect BRAF |
| 41 VS - 6766 +/ - FAKi Induces Significant Tumor Regression in KRAS G12V mt NSCLC in vivo Model, with Clear Differentiation from Trametinib Doses Tested Trametinib: 0.1 mg/kg QD (5 days/week) VS - 6766: 0.1 mg/kg QD (5 days/week) FAKi: 50 mg/kg BID (5 days/week) KRAS G12V mutant; Tp53 KO NSCLC • VS - 6766 monotherapy caused tumor regression • VS - 6766 + FAKi showed stronger regression • No significant anti - tumor effect of trametinib at same dose level Reference: Coma et al. AACR 2021 Vehicle Trametinib VS-6766 FAKi VS-6766 + FAKi 0 2 4 6 8 10 20 40 60 80 Tumor volume fold change (mean) 4 weeks of treatment Statistics: Mann - Whitney test Collaboration with Mariano Barbacid |
| 42 Case Study: Response to VS - 6766 + Defactinib in a Patient with KRAS G12V mutant NSCLC Reference: Krebs et al. AACR 2021 May 2019: Diagnosed with NSCLC June 2019 - Sept 2019: Treated with first line Carboplatin + Pemetrexed + Pembrolizumab Oct 2019: Progression, palliative RT to right hip Nov 2019 – present: On treatment in FRAME study VS - 6766 + Defactinib VS - 6766 + Defactinib |
| 43 Strong Signal Identified in KRAS G12V NSCLC Preclinical evidence suggests combination with Defactinib may improve efficacy in KRAS G12V mt NSCLC Activity of VS - 6766 as a single agent and in combo with Defactinib in KRAS G12V mt NSCLC VS - 6766 ± Defactinib Has Shown a 57% ORR in KRAS G12V mt NSCLC in Integrated Analysis References: 1 Guo, et al Lancet Oncology 2020 2 Krebs, AACR April 2021(March 18, 2021 cutoff) Best Response by RECIST in KRAS G12V mt NSCLC Time on Treatment for KRAS G12V mt NSCLC NSCLC (57% ORR; N=7) Weeks on treatment 0 10 20 30 40 50 60 70 Mono Combo Mono Mono Mono Combo Mono 180 200 220 216 216 70 70 58 58 20 20 19 19 18 18 14 14 Time on Treatment (weeks) Continuing on treatment Time on Treatment 24 Mono: VS-6766 monotherapy Combo: VS-6766 + Defactinib * * 4.0 mg VS-6766/200 mg defactinib + + |
| 44 VS - 6766 Monotherapy Has Shown Clinical Activity in Several RAS/RAF Mutant Cancer Indications, Including NSCLC and Gynecologic Cancers Confirmed responses especially in patients with KRAS G12V mutation Guo et al., Lancet Oncology 2020 KRAS G12R |
| 45 Overcoming Key Resistance Mechanisms to MEK Inhibitors • MEK inhibition induces compensatory activation of pFAK preclinically and clinically Pre dose Post VS-6766 Post combination 0 50 100 150 p-FAK H-Score o Trametinib induced ↑ pFAK (Y397) preclinically in KRAS mt NSCLC cell lines o Also observed in patients • VS - 6766 induced ↑ pFAK (Y397) as a potential resistance mechanism in the majority of patients • Combination with defactinib reduced this compensatory pFAK signal = Feedback Reactivation References: Banerji, BTOG Dublin, Jan 23, 2019 Banerji, AACR VM 1, April 27, 2020, CT143 |
| 46 VS - 6766 and FAK Inhibitor Combination Leads to More Robust Anti - Tumor Efficacy in vivo KRAS mt Ovarian TOV - 21G in vivo Model 1 KRAS mt NSCLC H358 in vivo Model 2 0 5 10 15 0 100 200 300 400 500 Tumor growth VS-4718 + CH5126766 Days on treatment Tumor volume (mm 3 +/- SEM) Vehicle Trametinib 1.5 mg/kg QD FAKi 50 mg/kg BID VS-6766 1.5 mg/kg QD VS-6766 + FAKi 0 5 10 15 20 0 200 400 600 Tumor growth Days on treatment Tumor volume (mm 3 +/- SEM) Vehicle Trametinib 0.3 mg/kg QD FAKi 50 mg/kg BID VS-6766 0.3 mg/kg QD VS-6766 + FAKi References: 1 Coma AACR 2021; 2 Krebs AACR 2021 |
| 47 0 10 20 30 40 50 60 70 FRA101017 FRA102005 FRA101043 FRA101040 FRA101034 FRA101018 FRA102016 FRA101026 FRA101020 FRA101036 FRA102004 FRA101010 FRA102008 FRA102006 FRA102007 FRA101041 FRA102017 FRA102002 FRA102009 FRA101031 Time on Treatment (weeks) Unconfirmed PR Partial response Stable disease Progression disease Time on Treatment G12D Q61H G12C G12D G12A G12V G12D G12C G12D G12C G12D G12C G12D G12V * Time to response * * G12A G12C G12D Continuing on treatment * # # G12C G12D G12C NSCLC Responses with VS - 6766 + Defactinib Combination (FRAME) (n=20) Confirmed responses in 2/2 patients with KRAS G12V mt NSCLC Tumor reduction in 4/6 patients with KRAS G12C mt NSCLC Data cut off March 5, 2021 • ORR = 15% (3/20) • ORR in G12V mt = 100% (2/2) • DCR =65% (13/20) • 3/20 (15%) still on study • 7 pts on treatment ≥ 24 weeks Reference: Krebs et al. AACR 2021 FRA101040 FRA101034 FRA101026 FRA101017 FRA101018 FRA101043 FRA102008 FRA101020 FRA102007 FRA102017 FRA102004 FRA102006 FRA102002 FRA102009 FRA101036 FRA101041 FRA101010 FRA101031 -80 -70 -60 -50 -40 -30 -20 -10 0 10 20 30 40 50 60 Best Response (% change from baseline) G12C G12D G12A G12D G12D Q61H G12C G12A G12C * G12C G12V G12C G12V G12D Continuing on treatment * Partial response Stable disease Best response by RECIST in KRAS mt NSCLC Progressive disease * * G12D G12D G12C # # Unconfirmed PR G12D KRAS mt FRA101040 FRA101034 FRA101026 FRA101017 FRA101018 FRA101043 FRA102008 FRA101020 FRA102007 FRA102017 FRA102004 FRA102006 FRA102002 FRA102009 FRA101036 FRA101041 FRA101010 FRA101031 -80 -70 -60 -50 -40 -30 -20 -10 0 10 20 30 40 50 60 Best Response (% change from baseline) G12C G12D G12A G12D G12D Q61H G12C G12A G12C * G12C G12V G12C G12V G12D Continuing on treatment * Partial response Stable disease Best response by RECIST in KRAS mt NSCLC Progressive disease * * G12D G12D G12C # # Unconfirmed PR G12D KRAS mt |
| 48 Target exposures for preclinical tumor regression & pERK inhibition in human subjects are covered by twice weekly dosing of 3.2 mg VS - 6766, 3 wks on/1 wk off Modeling of PK for 3 .. 2 mg VS - 6766 2 /wk, 3 wks on/ 1 wk off, based on 3 .. 2 mg single dose PK data (study CCR 3808 ) Relationship to average exposure for tumor regression in KRAS G 12 V mt NSCLC mouse model and IC 50 against human PBMC pERK activity References: Martinez - Garcia et al., Clin Cancer Res 2012; Coma et al. AACR 2021 C av for tumor regression from KRAS G12V mt NSCLC GEMM model IC 50 for pERK inhibition in PBMCs of orally dosed subjects |
| 49 VS - 6766 Upregulates MHC Class I Antigens on Tumor Cells: a mechanism for potentiation of I/O efficacy Cell Line Tumor type RAS/RAF mutation status A549 Lung KRASmt G12S TOV21g Ovarian KRASmt G13C SKMEL5 Melanoma BRAFmt V600E IGR - 1 Melanoma BRAFmt V600E WM115 Melanoma BRAFmt V600E A549 TOV21g SKMEL5 IGR1 WM115 0 2 4 6 B2M Relative mRNA Levels DMSO VS-6766 KRAS mt BRAF mt A549 TOV21g SKMEL5 IGR1 WM115 0 2 4 6 8 HLA-A Relative mRNA Levels DMSO VS-6766 KRAS mt BRAF mt A549 TOV21g SKMEL5 IGR1 WM115 0 2 4 6 TAP-1 Relative mRNA Levels DMSO VS-6766 KRAS mt BRAF mt A549 TOV21g SKMEL5 IGR1 WM115 0.0 0.5 1.0 1.5 2.0 2.5 TAP-2 Relative mRNA Levels DMSO VS-6766 KRAS mt BRAF mt VS - 6766 @ 1 µM (except SKMEL5 and IGR - 1, 300 nM) Reference: Pachter, RAS - Targeted Drug Development, Sept 2020 |
| 50 VS - 6766 Enhances Tumor Growth Inhibition when Combined with Anti - PD - 1 in the CT26 KRAS (G12D) Syngeneic Model Reference: Pachter, RAS - Targeted Drug Development, Sept 2020 -100 0 100 200 300 400 Response at Day 13 % Change in Tumor Volume Vehicle VS-6766 anti-PD-1 VS-6766 + anti-PD-1 20 40 60 80 100 0 20 40 60 80 100 Survival Time Percent survival VS-6766 + anti-PD-1 anti-PD-1 3 mg/kg 2xW x 4 doses VS-6766 0.5 mg/kg QD x 28 days Vehicle Day 11, Last dose anti - PD - 1 Day 28, Last dose VS - 6766 0 10 20 30 40 50 60 70 80 90 100 0 20 40 60 80 100 Survival Days after first dose Percent survival VS-6766 + anti-PD-1 anti-PD-1 3 mg/kg 2xW x 4 doses VS-6766 0.5 mg/kg QD x 28 days Vehicle Tumor re - challenge in tumor - free mice showed immune memory with increased memory T cells 0 2 4 6 8 10 12 14 16 18 20 22 24 26 0 200 400 600 800 1000 1200 Tumor growth Days after first dose Tumor volume (mm 3 +/- SEM) Vehicle VS-6766 0.5 mg/kg QD anti-PD-1 3 mg/kg 2xW VS-6766 + anti-PD-1 |
| 51 Experienced Senior Management Team Brian Stuglik Chief Executive Officer Cathy Carew Chief Organizational Effectiveness Officer Louis Denis, M.D. Chief Medical Officer Principal – HR Collaborative Ironwood, ActiveBiotics, Dynogen, Tufts Health Plan CMO, Asana BioSciences Boehringer - Ingelheim, Pfizer Global VP & Chief Marketing Officer – Lilly Oncology Founding Member – Proventus Health Solutions Daniel Paterson President and Chief Operating Officer Jonathan Pachter, Ph.D. Chief Scientific Officer CEO – The DNA Repair Co. (now On - Q - ity) PharMetrics (now IMS), Axion Head of Cancer Biology – OSI (now Astellas) Schering - Plough Rob Gagnon Chief Business and Financial Officer CFO – Harvard Bioscience, Clean Harbors VP of Finance – Biogen Idec Hagop Youssoufian, MSc, M.D. Head of Medical Strategy CMO, BIND Therapeutics, EVP, Progenics, CMO & EVP, Ziopharm Oncology, SVP, Imclone |
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