Corporate Presentation January 2024 Exhibit 99.1

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Aura Biosciences – Developing a Novel Class of Drugs in Oncology Ocular Oncology Franchise Choroidal Melanoma – Global Ph 3 CoMpass Trial: FPI was Q4 2023 Most US sites activated; Trial actively enrolling Special Protocol Assessment (SPA) Agreement with FDA Ph 3 assumptions supported by Ph 2 data Choroidal Metastasis – Ph 2 trial planned to initiate in mid-2024 Second ocular indication potentially doubles market opportunity Urologic Oncology Franchise Clinical complete response in first patient with single dose Data supports dual mechanism Updated protocol includes both NMIBC and MIBC Strong Cash Position Current cash runway expected to fund operations into 2H 2026

Pipeline Targeting Life-Threatening Cancers with High Unmet Needs Program OTHER SOLID TUMORS OCULAR ONCOLOGY Preclinical Phase 1 Phase 2 Phase 3 Planned Milestones Choroidal Metastasis (Multiple primary cancers with metastasis in the eye e.g., Breast and Lung) Primary Choroidal Melanoma (Suprachoroidal administration) Cancers of the Ocular Surface Bladder Cancer (NMIBC and MIBC) Other HSPG* Expressing Tumors (e.g., Cutaneous melanoma, HNSCC) YE 2024 – Ph 2 data Mid-2024 – Ph 1 data Global Commercial Rights for All Product Candidate Indications *VDCs bind to a subset of modified tumor associated glycosaminoglycans (GAGs) that are part of the heparan sulphate chain of HSPGs. Schiller et al. Viruses 2022, 14(8), 1656 YE 2024 – Majority of sites activated globally

Targeted Oncology Platform: Virus-Like Drug Conjugates (VDCs) Virus-Like Particle Conjugated to a Cytotoxic Payload Selective Binding to Tumor Associated HSPGs* Virus-Like Particle (VLP) Virus-Like Drug Conjugate (VDC) Cx Cytotoxic Drug Kines et al; International Journal of Cancer, 138;901–911, February 2016; Kines et al; Molecular Cancer Therapeutics, 17(2) February 2018; Kines et al; Cancer Immunology Research, May 2021 Potential Key Differentiation: Potency, Multivalent Binding and Selectivity Potential Treatment of Multiple Solid Tumors *VDCs bind to a subset of modified tumor associated glycosaminoglycans (GAGs) that are part of the heparan sulphate chain of HSPGs. Schiller et al. Viruses 2022, 14(8), 1656

Bel-sar is a VDC with a Novel Dual Mechanism of Action Light Activatable Drug Bel-sar Bel-sar is a novel VDC that consists of VLP conjugated to ~200 molecules of light activatable drug Potential Key Differentiation: Agnostic to Genetic Mutations, Less Susceptible to Resistance Mechanisms, Long Term Anti-tumor Immunity Kines et al; Cancer Immunology Research, May 2021 VDCs bind to a subset of modified tumor associated glycosaminoglycans (GAG) that are part of the heparan sulphate chain of HSPGs. Schiller et al. Viruses 2022, 14(8), 1656 Bel-sar – Belzupacap Sarotalocan Reactive oxygen species disrupts cell membrane and organelles

Ocular Oncology Franchise Bel-sar INN: belzupacap sarotalocan Target Indications: Early-Stage Choroidal Melanoma Choroidal Metastasis Other Ocular Cancers

Primary Choroidal Melanoma—High Unmet Medical Need Choroidal Melanoma is a Rare and Life-Threatening Primary Ocular Cancer with No Drugs Approved 1. Heiting, G. Iris/uvea of the eye. Accessed Oct. 3, 2023. https://www.allaboutvision.com/en-gb/resources/uvea-iris-choroid/ 2. Kaliki S, Shields CL. Uveal melanoma: relatively rare but deadly cancer. Eye (Lond). 2017;31(2):241-257. doi:10.1038/eye.2016.275 3. Clearview & Putnam & Assoc. Market Research Most common primary intraocular cancer in adults2 Impacts ~11,000 patients in US/Europe per year3 ~80% patients diagnosed with early-stage disease3 Choroid is 90% of the uvea1 50% of patients develop metastasis within 15 years (metastatic uveal melanoma)2 choroid Uvea: Choroid, Ciliary Body and Iris

Radiotherapy is the Standard of Care for Early-Stage Disease  Surgical treatment of large choroidal melanoma with ruthenium 106 plaque brachytherapy2 1. Up To Date. Plaque brachytherapy for uveal melanoma. Accessed September 20, 2023. https://ykhoa.org/d/image.htm?imageKey=ONC/116591 2. Kim M, Lee SM, Lee. Surgical treatment of a large choroidal melanoma. American Academy of Optometry 2022 Video Program. March 1, 2023. Accessed September 20, 2023. https://www.aao.org/education/annual-meeting-video/surgical-treatment-of-large-choroidal-melanoma 3. Peddada KV, Sangani R, Menon H, Verma V. Complications and adverse events of plaque brachytherapy for ocular melanoma. J Contemp Brachytherapy. 2019;11(4):392-397. doi:10.5114/jcb.2019.87407 (A) Schematic drawing showing a Collaborative Ocular Melanoma Study (COMS) style plaque sutured to the sclera overlying the location of the uveal melanoma1 (B) Intraoperative photograph showing COMS plaque in place. The plaque stays in place for 3 to 7 days1 Plaque Brachytherapy for Choroidal Melanoma Requires Invasive Surgery with Irreversible Complications and Does Not Prevent Metastasis Maculopathy, Cataract, Glaucoma, Vitreous Detachment and Vitreous Hemorrhage are Common Vision Threatening Complications of Brachytherapy3

Up to 87% of Patients Will be Blinded when Treated with Radiotherapy Jarczak J, Karska-Basta I, Romanowska-Dixon B. Deterioration of visual acuity after brachytherapy and proton therapy of uveal melanoma, and methods of counteracting this complication based on recent publications. Medicina (Kaunas). 2023;59(6):1131. Tsui I, Beardsley RM, McCannel TA, Oliver SC, et al. Visual acuity, contrast sensitivity and color vision three years after iodine-125 brachytherapy for choroidal and ciliary body melanoma. Open Ophthalmol J. 2015;9:131-5. Normal Vision Vision with Reduced Contrast Sensitivity Visual Acuity, Color Vision and Contrast Sensitivity Decrease Significantly within the First Year after Treatment and Continue to Decrease Over Time Proportion of Patients Legally Blind (BCVA ≤20/200) after Brachytherapy1,2 Contrast Sensitivity Diminishes Significantly within the First Year after Brachytherapy and Continues to Deteriorate Over Time2

Benefit/Risk of Radiotherapy Drives “Watch and Wait” in Patients with Early-Stage Disease Brachytherapy: Vision Loss/Blindness Clearview & Putnam & Assoc. Market Research Kaliki S, Shields CL. Uveal melanoma: relatively rare but deadly cancer. Eye (Lond). 2017;31(2):241-257. doi:10.1038/eye.2016.275 (~8,000)1 (~2,300)1 (~2,000)2 Indeterminate Lesions and Small PCM Medium/large PCM Prevalence: Patients US/EU Early Stage Late Stage Metastatic Disease Systemic Chemotherapy (KIMMTRAK) Current Treatment Watch and Wait/Brachytherapy Most of the Addressable Population is Untreated Due to Risk of Treatment Related Vision Loss and Severe Comorbidities inevitably

Bel-sar has the Potential to be the First Approved Therapy in Primary Choroidal Melanoma No radiation-related morbidity Preservation of vision Local tumor control Opportunity to treat early and reduce risk of metastases Improvement in safety and quality of life Bel-sar is Delivered by Simple Suprachoroidal Injection Goals of Treatment suprachoroidal Light Activation with Standard Ophthalmic Laser Bel-sar – Belzupacap Sarotalocan 3-4h In-Office Two injections (2min. each) 30 min apart 10-30 min. procedure

Ocular Oncology Franchise Represents a Multi-Billion Dollar Addressable Market Opportunity Ocular Oncology Franchise total addressable market (US/EU) ~80% of patients are diagnosed at the early stage (indeterminate lesions (ILs) and small tumors) Current radiotherapy treatment Leaves ~87% of patients with major irreversible vision loss ~100 Ocular Oncologists in US/EU — focused call point <20 Field Based Team Intend to add small sales force to launch globally ~66,000 patients/year 1. ClearView & Putnam & Assoc. Epidemiology Analysis Choroidal Melanoma and Choroidal Metastasis 2. American Cancer Society- Retinoblastoma statistics 3. Includes Conjunctival Melanoma, Primary Acquired Melanosis, Squamous Cell Carcinoma and Ocular Surface Squamous Neoplasia (https://pubmed.ncbi.nlm.nih.gov/12788119/; https://pubmed.ncbi.nlm.nih.gov/19628487/; https://pubmed.ncbi.nlm.nih.gov/8676629/; https://pubmed.ncbi.nlm.nih.gov/29511061/; https://pubmed.ncbi.nlm.nih.gov/9037556/) Multibillion $ addressable market opportunity Cancers of the Ocular Surface Retinoblastoma Choroidal Melanoma Choroidal Metastasis 11,000 patients/year1 20,000 patients/year1 500 patients/year2 ~35,000 patients/year3 11,000 patients/year Choroidal Melanoma patients diagnosed each year (US/EU)

Bel-Sar has the Potential to be an Effective, Vision Preserving Therapy for the Treatment of Indeterminate Lesions and Small Choroidal Melanoma Potential Future Patient Treatment Journey Treatment First Line Treatment with Bel-sar Brachytherapy: Vision Loss/Blindness (~8,000)1 (~2,300)1 (~2,000)2 Indeterminate Lesions and Small PCM Medium/large PCM Early Stage Late Stage Metastatic Disease Chemotherapy (KIMMTRAK) Prevalence: Patients US/EU Clearview & Putnam & Assoc. Market Research Kaliki S, Shields CL. Uveal melanoma: relatively rare but deadly cancer. Eye (Lond). 2017;31(2):241-257. doi:10.1038/eye.2016.275

Adverse Event or Adverse Reactions KIMMTRAK1 Darovasertib + Crizotinib Combination2 Bel-Sar Any Grade Any Grade Any Grade* Cytokine release syndrome 89% Not Disclosed 0% Rash 83% Not Disclosed 0% Pyrexia 76% Not Disclosed 0% Pruritus 69% Not Disclosed 0% Fatigue 64% 40% 0% Nausea 49% 79% 0% Vomiting Not Disclosed 52% 0% Chills 48% Not Disclosed 0% Hypo-/hyperpigmentation 47% Not Disclosed 0% Abdominal pain 45% Not Disclosed 0% Edema 45% 57% 0% dermatitis acneiform Not Disclosed 44% 0% Hypotension Not Disclosed 34% 0% Hypoalbuminemia Not Disclosed 32% 0% Dizziness Not Disclosed 28% 0% Immunocore Corporate Presentation. 2023 August. Slide 12. https://ir.immunocore.com/static-files/415c5cbb-caae-4ffa-9beb-cb40cfcfc132 IDEAYA Clinical Update Presentation. 2023 April 24. Slide 10. Note: Certain data in this presentation are based on a cross-trial comparisons and are not based on any head-to-head clinical trials. Cross-trial comparisons are inherently limited and may suggest misleading similarities or differences. Results of head-to-head comparisons may differ significantly from those set forth herein. *Safety outcomes for ongoing Ph2 study with bel-sar presented on slide 22 Bel-sar has Demonstrated a Favorable Profile for the Treatment of Early-Stage Disease

Choroidal Melanoma Ph 2 Data

Ph 2 Trial – Dose Escalation and Expansion with Suprachoroidal Administration Trial Design – Enrollment Complete (n=22) *Cohort 2: 2 subjects were planned; third subject was additionally enrolled due to dose error in 1 subject **12 patients enrolled, 1 subject who discontinued after 1 cycle due to unrelated SAEs is not included in data analysis (n=11). Data that follows will be based on a cohort of 11 ClinicalTrials.gov Identifier: NCT04417530 ; AU-011-202 Goal: To Determine Safety, Optimal Dose and Therapeutic Regimen with Suprachoroidal Administration One Cycle = Doses on days 1, 8, and 15 1 dose- 20 μg x 1 Laser 1 dose- 40 μg x 1 Laser 1 dose- 40 μg x 2 Lasers 2 doses- 40 μg x 2 Lasers QWx2 Cohort 1 (n=1) Cohort 2 (n=3*) Cohort 3 (n=2) Cohort 4 (n=3) Cohort 5 9 doses- 80 μg x 2 Lasers QWx3 3 cycles Cohort 6 (n=up to 10) 2 Cycles (n=1)3 Cycles (n=2) N=10 1- 2 Doses (n=9); 2 cycles-6 doses (n=1) N=11** 3 Cycles (9 doses) 6-9 doses- 40 μg x 2 Lasers QWx3 Up to 3 cycles Patient Population Representative of Early-Stage Disease: Indeterminate Lesions and Small Choroidal Melanoma Endpoint Endpoint Definitions Tumor Progression Growth in Tumor Height ≥0.5mm or ≥1.5 mm in Largest Basal Diameter (LBD) Visual Acuity Loss Decrease from Baseline: ≥15 letters Tumor Thickness Growth Rate Change in Rate of Growth of Tumor Thickness Subtherapeutic Regimens Therapeutic Regimen

High Tumor Control Rates Observed – Durable at 12 Months Follow Up Dose Response: Subtherapeutic vs Therapeutic Regimen Tumor Progression: change from baseline in thickness ≥0.5mm; or in LBD ≥1.5mm confirmed by at least one repeat assessment August 3, 2023, data on file Aura Biosciences Dose/Regimen Total Patients (n) Total Patients (n) Tumor Control Rate Subtherapeutic Regimens Single dose up to 2 cycles 10 20% (2/10) Therapeutic Regimen 3 Cycles (n=11) 11 73% (8/11) 3 Cycles and Ph 3 eligible (n=10)* 10 80% (8/10) >90% Completed 12 Months * One subject with circumpapillary tumor that did not meet Ph 3 criteria is not included High Tumor Control Rates with Therapeutic Regimen in Ph 3 Eligible Patients with Active Growth

High Tumor Control Rates Observed in Ph 3 Population Treated with Therapeutic Regimen August 3, 2023, data on file Aura Biosciences Active Growth and 3 Cycle Regimens (n=10) Progression Definition based on Tumor Thickness (Increase ≥0.5mm) Change from Baseline in Tumor Thickness Over 12 Months Treatment Timeframe Follow-up Timeframe Change from Baseline in Tumor Thickness (mm) Subtherapeutic Regimens (n=10) Ph 2 Interim Data Demonstrated Tumor Control Rate of 80%, with 90% of Patients at 12 Months of Follow Up Change from Baseline in Tumor Thickness (mm) Patients who had documented growth at entry (n=6) Patients who did not have documented growth at entry (n=4) Progression Definition based on Tumor Thickness (Increase ≥0.5mm) Change from Baseline in Tumor Thickness Over 12 Months Treatment Timeframe Follow-up Timeframe Patients who had documented growth at entry (n=10)

Ph 2 Interim Data Demonstrated a Negative Growth Rate Post-Treatment Successful Treatment with 3 Cycle Regimen in Ph 3 Eligible Tumors with Active Growth Change in Tumor Growth (mm/yr) (n=8) August 3, 2023, data on file Aura Biosciences Tumor thickness growth rates/ slopes estimated using Mixed Models for Repeat Measures (random intercept and slope model for Historical and Study periods) Interim Data Showed Negative Growth Rate Among Responders in Planned Ph 3 Population (P <0.0001) Rate of Tumor Growth (mm/yr) + 0.383 - 0.001 P < 0.0001

. Populations Total Patients (n) Vision Failures (n) Vision Preservation Rate All Dose Cohorts All Treated Patients 22 1 96% Subtherapeutic Single dose up to 2 cycles 10 0 100% Therapeutic Regimens 3 Cycles (n=11) 11 1 91% 3 Cycles and Ph 3 eligible (n=10)* 10 1 90% >90% Patients Completed 12 months 90% Visual Acuity Preservation Despite 80% of These Patients Being at High Risk for Vision Loss *One subject with circumpapillary tumor that doesn’t meet Ph 3 criteria is not included August 3, 2023, data on file Aura Biosciences Vision acuity loss definition based on ETDRS BCVA letter score (≥ 15 letters from baseline) 90% Visual Acuity Preservation Data Supports Potential to be Front Line Therapy for Early-Stage Disease Median Change in BCVA in Ph3 Eligible Patients with Therapeutic Regimen (n=10) Vision Loss (15 letters) Months

Ph 2 Safety Data Supports Potential to be First Line Treatment in Early-Stage Disease No Posterior Inflammation, No SAEs and No Grade 3 Related Adverse Events *J. Contemp Brachytherapy. J. 2019 Aug; 11(4): 392–397.; Arch Ophthalmol. 2000;118(9):1219-1228; Curr. Opin. Ophthalmol. 2019 May;30(3):206-214; Eye 2017 Feb;31(2):241-257 **High-Risk Vision Loss (HRVL) are those subjects with tumors <3mm to fovea or optic nerve Adverse Event Radiotherapy* Bel-Sar+ Surgeries secondary to AEs+ (e.g., Cataracts) 40%+ 0% Radiation Retinopathy 40%+ 0% Neovascular Glaucoma 10% 0% Dry Eye Syndrome 20% 0% Strabismus 2%+ 0% Retinal Detachment 1-2% 0% Vision Loss (≥15 letters) ~70% ~5% Serious Adverse Event Radiotherapy* Bel-Sar+ Scleral Necrosis 0-5% 0% Enucleation/Eye Loss 10-15% 0% Severe Vision Loss (≥30 letters) in HRVL** ~90% 0%++ * Certain data in this presentation are based on a cross-trial comparisons and are not based on any head-to-head clinical trials. Cross-trial comparisons are inherently limited and may suggest misleading similarities or differences. Results of head-to-head comparisons may differ significantly from those set forth herein. +Related to bel-sar or laser **73% (16/22) of patients in Ph2 SC trial were at high risk for vision loss Bel-Sar – Belzupacap Sarotalocan; AEs – Adverse Events; SAEs – Serious Adverse Events Table presents percentage of subjects with AEs related to bel-sar or laser by severity and overall; subjects with more than 1 AE are counted in the highest severity group *Treatment-emergent AEs related to bel-sar or laser in 1 patient each or <5% (anisocoria, conjunctival edema, cystoid macular edema, pupillary reflex impaired, retinal pigment epitheliopathy, salivary gland enlargement) August 3, 2023, data on file Aura Biosciences Ongoing Ph 2 Safety Outcomes with SC Administration All Treated Subjects (n=22) Drug/Laser Related Adverse Events >5% Subjects* Grade I Grade II Grade III Total Anterior Chamber Inflammation 18% 0 0 18% Anterior Chamber Cell 9% 0 0 9% Eye Pain 9% 0 0 9%

Randomized Controlled Global Ph 3 Trial

SPA Agreement with FDA Supports Global Ph 3 Trial Design Fast Track and Orphan Designations Time to Composite Endpoint: Tumor Progression or Visual Acuity Failure Time to Tumor Progression Primary Endpoint First Key Secondary Endpoint Enrollment (N=~100) Randomize (2:1:2) 80 µg bel-sar treatment arm (n=40) 40 µg bel-sar treatment arm (n=20) Sham control arm (n=40) 15 Month Primary Efficacy Analysis An SPA Indicates Concurrence by the FDA that the Design of the Trial can Adequately Support a Regulatory Submission SPA Agreement SPA – Special Protocol Assessment; Bel-sar – Belzupacap Sarotalocan

Note: Subjects either had an event or were censored at the last visit. Some subjects had Week 52 visit after 365 days. Time to Composite Endpoint is defined as time to tumor progression or vision acuity failure, whichever occurs earlier. Tumor progression is defined as a change from baseline in thickness ≥0.5mm; or in LBD ≥1.5mm confirmed by at least one repeat assessment. Log-rank test p-value based on unsimulated original KM curves August 3, 2023 data on file Aura Biosciences Study duration 12 months. Some patients presented delayed for their final 12-month visit. Any events at the final visit are assigned to the actual time of that visit. Time to Composite Endpoint Days 3 cycles (n=10) Subtherapeutic up to 2 cycles (n=10) Log-rank test p=0.0023 Time to Tumor Progression Days 3 cycles (n=10) Subtherapeutic up to 2 cycles (n=10) Progression-Free Probability Log-rank test p=0.0012 Ph 2 Interim Data Supports Assumptions for the Potential Success of Ph 3 with High Statistical Significance Kaplan-Meier Analysis Simulation of Key Primary & Secondary Endpoint with Ph 2 Data Progression-Free Probability

Clinical Endpoints to Support Potential Approval in Alignment with Regulatory Agencies Note: Tumor Height is synonymous with Tumor Thickness; VA – Visual Acuity; Bel-sar – Belzupacap Sarotalocan Conservative Assumptions Provide Support for Potential for Regulatory Submission (Phase 3 Trial Powered at 95%) 45% 13% Tumor Progression Assumptions Visual Acuity Loss Assumptions Endpoint Endpoint Assumptions Endpoint Definitions Tumor Progression Bel-sar: 35% Tumor Progression Sham: 80% Tumor Progression Growth in Tumor Height ≥0.5mm or ≥1.5 mm in Largest Basal Diameter Vision Acuity Loss Bel-sar: 15% VA Loss Sham: 2% VA Loss Decrease from baseline: ≥15 letters Composite Endpoint

Choroidal Metastasis

Choroidal Metastasis is a High Unmet Medical Need Choroidal Metastasis Cause Decreased Vision and Decreased Quality of Life in Patients Fighting Metastatic Cancer Originate from Multiple Primary Cancers (e.g., Breast and Lung) 20,000/year eyes diagnosed in US 88% with choroidal location 72% unilateral and solitary Standard of Care is Daily Radiotherapy for 4 Weeks Vision Loss and Radiation Associated Complications Breast 40-53% Lung 20-29% GI 4% Kidney 2% Prostate 2% Skin 2% Mathis et al. New concepts in choroidal metastasis, Progress in retinal and eye research (2019), Cohen, Ocular metastasis, Eye (2014), Shields et al. Survey of 520 eyes with uveal metastases. Ophthalmology (1997), Namad et al. Bilateral choroidal metastasis from non-small lung cancer, Case reports in oncological medicine (2014).

Choroidal Metastasis – Ph 2 Trial Design Cohort 1a N=3 patients 80µg x 2L 1 cycle Cohort 2a N=3 patients 140µg x 2L 1 cycle Cohort 3a N=3 patients 200µg x 2L 1 cycle Cohort 3b N=3 patients 200µg x 2L 2 cycles Safety/DLT Efficacy Choroidal Tumor Progression Choroidal Tumor Growth rate Vision improvement Study Objectives Study Population Patients with unilateral, unifocal choroidal metastasis Breast or Lung Primary No changes in concurrent systemic medications planned Study Design (n=12*) *3+3 Design. Each cohort will have a minimum of 3 and a maximum of 6 patients. Highlights: Primary Endpoint at one-month post-treatment; possibility to see tumor shrinkage; FDA oncology division

Urologic Oncology Bel-sar INN: belzupacap sarotalocan Target Indications: Bladder Cancer

Bladder Cancer is a High Unmet Medical Need Non-Muscle Invasive Bladder Cancer Muscle Invasive Bladder Cancer ~500,000 New cases/ year globally1 61,000 New cases/year in the US1 New cases/year in the US2 20,000 Putnam & Assoc. Epidemiology Analysis Campbell-Walsh-wein Urology, 2021, 12th edition ; Chapter 137, page 3112  Recurrence, multiple TURBT surgeries, Progression of Disease, Loss of Bladder/Cystectomy Progression of Disease, Loss of Bladder/Cystectomy, Metastasis and Survival Unmet Need Unmet Need

Intravesical gene therapy (Adstiladrin) Systemic Immunotherapy (Keytruda) INTRAVESICAL CHEMOTHERAPY Bel-Sar has the Potential to be Front Line in Conjunction with TURBT Presents with Blood in urine TURBT BCG >6 tx INTRAVESICAL CHEMOTHERAPY Adding Bel-sar can potentially: Prevent Recurrence after TURBT Avoid need for Adjuvant Chemotherapy Avoid need for six cycles of BCG Increase efficacy of checkpoint inhibitors Prevent Cystectomy Urologist Adjuvant Therapy BCG Refractory Intravesical gene therapy (Adstiladrin) Systemic Immunotherapy (Keytruda) Cystectomy Illustrative Current Bladder Cancer Patient Journey Diagnosis and Treatment Plan Illustrative Future Bladder Cancer Patient Journey Presents with Blood in urine BCG >6 tx Urologist Adjuvant Therapy BCG Refractory Cystectomy Diagnosis and Treatment Plan Bel-sar + TURBT recurrence BCG – Bacillus Calmette Guerin; TURBT- transurethral resection of bladder tumor

Bel-Sar has a Unique MoA Designed for the Local Treatment Bel-sar’s Local Administration is Aligned with Clinical Practice and has a Unique MoA that may be Complementary to Other Drugs Local Administration and Light Activation with Standard Cystoscope Bel-sar, AU-011 – Belzupacap Sarotalocan MoA – Mechanism of Action Bladder injections are common and simple to perform (e.g., Botox) Precedent for immune-mediated tumor clearance (BCG & checkpoint inhibitors) Local delivery in the bladder increases tumor drug exposure Lasers are commonly used by urologists Treatment Aligned with Current Urologic Oncology Practice

Ph 1 Trial Designed to Evaluate Safety, Feasibility and MoA Ph 1 Data is Anticipated to Support the Future Development of Bel-sar for the Treatment of Both NMIBC and MIBC Study Design and Objectives (n=19) Bel-sar Alone Part 1 (n=5) Part 2 (n=14) Bel-sar + Focal Light Activation Completed Ongoing Safety/DLT Feasibility of technique Focal distribution of bel-sar Focal necrosis Markers of immune activation TURBT and cystectomy patients NMIBC and MIBC patients No treatment-related adverse events, serious adverse events or dose-limiting toxicities were seen for the 5 patients Study Objectives Study Population Bel-sar – Belzupacap Sarotalocan MoA – Mechanism of Action TURBT- transurethral resection of bladder tumor

Clinical Complete Response with Immune Activation after Single Dose Confirmed by Histopathology Day 1 Diagnostic biopsy shows non- invasive, low grade urothelial carcinoma Injection of Bel-sar (100ug) performed within tumor and below tumor (Aura present w/ Urologist) DAY 1 Biopsy Day 2 Urologist performs Light activation with 689nm infrared light (50J/cm2) (~5 min duration) (Aura present w/ Urologist) Day 9 Urologist performs TURBT in area where tumor used to be present. Biopsy shows denuded urothelial mucosa, no cancer cells; focal ulcer and chronic inflammation (eosinophils/lymphocytes) TURBT Biopsy Day 1 Day 1 Day 2 Day 3-7 Bel-sar, AU-011 – Belzupacap Sarotalocan TURBT- transurethral resection of bladder tumor Day 9 Ph 1- Preliminary Data Light Activated Cohort (n=1)

Pre-Treatment Biopsy (Day 1) shows Papillary Urothelial Carcinoma Papillary urothelial carcinoma Example of papillary carcinoma (Ta) H&E Stain Pre-injection bladder biopsy demonstrating low-grade papillary urothelial carcinoma; non-invasive.

Clinical Complete Response Confirmed by Histopathology after Single Dose of Bel-sar Disappearance of all cancer cells after treatment and immune activation Post-treatment TURBT demonstrating necrosis, inflammatory infiltrate, and no residual carcinoma. Circled region shows area of necrosis; arrow indicates edge of inflammatory infiltrate. necrosis Immune infiltrate Evidence of Complete Response by Absence of Tumor Cells after Single Dose Treatment in First Patient

Durable CRs with Single Administration of Bel-sar in Bladder Cancer Model Data Demonstrated Robust Nonclinical Activity Supporting Development of Bel-sar as Single Agent and in Combination with Checkpoint Inhibitors Syngeneic Mouse Tumor Bladder Model (MB49 Model in C57BL/6 Mice) (N=8 -10/group) Tumor Regression Survival Survival After Re-challenge AU-011 Single Dose Treatment of Tumor Caused Anti-Tumor Immune Response and Prevented Tumor Growth After Re-Challenge Bel-sar: Belzupacap Sarotalocan; CR: Complete Responses Kines et al. Can Immunol Res 9(6):693-706, 2021

Strategy & Key Milestones

Aura Biosciences – Developing a Novel Class of Drugs in Oncology Ocular Oncology Franchise Choroidal Melanoma – Global Ph 3 CoMpass Trial: FPI was Q4 2023 Most US sites activated; Trial actively enrolling Special Protocol Assessment (SPA) Agreement with FDA Ph 3 assumptions supported by Ph 2 data Choroidal Metastasis – Ph 2 trial planned to initiate in mid-2024 Second ocular indication potentially doubles market opportunity Urologic Oncology Franchise Clinical complete response in first patient with single dose Data supports dual mechanism Updated protocol includes both NMIBC and MIBC Strong Cash Position Current cash runway expected to fund operations into 2H 2026