Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the following Registration Statements:

- Registration Statements on Form S-3 (Nos. 333-220053, 333-220054 and 333-226756) of Invitae Corporation and the related prospectuses

- Registration Statements on Form S-3ASR (Nos. 333-230053, 333-233109, 333-233110, 333-234768 333-237268, 333-237758, 333-237759, 333-253653, 333-253655, 333-255765, 333-256683 and 333-259550) of Invitae Corporation and the related prospectuses

- Registration Statement on Form S-4 (No. 333-220447) of Invitae Corporation, including any post-effective amendments thereto on Form S-3 or Form S-8

- Registration Statement on Form S-4 (No. 333-240137) of Invitae Corporation, including any post-effective amendments thereto on Form S-8

- Registration Statement on Form S-8 (No. 333-202066) pertaining to the 2015 Stock Incentive Plan, the Employee Stock Purchase Plan, and the 2010 Stock Incentive Plan of Invitae Corporation

- Registration Statements on Form S-8 (Nos. 333-216761, 333-223455 ,333-229972, 333-236799 and 333-253615) pertaining to the 2015 Stock Incentive Plan and the Employee Stock Purchase Plan of Invitae Corporation

- Registration Statement on Form S-8 (Nos. 333-232208, 333-237073, 333-240360, 333-249894, 333-255770, 333-258654 and 333-259512) pertaining to the 2015 Stock Incentive Plan of Invitae Corporation

- Registration Statement on Form S-8 (Nos. 333-261920) pertaining to the Stratify Genomics Inc. 2018 Incentive Plan

of our reports dated March 1, 2022, with respect to the consolidated financial statements of Invitae Corporation and the effectiveness of internal control over financial reporting of Invitae Corporation included in this Annual Report (Form 10-K) of Invitae Corporation for the year ended December 31, 2021.

/s/ Ernst & Young LLP

Redwood City, California

March 1, 2022