Exhibit 16.1

July 3, 2013

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549-7561

Dear Sirs/Madams:

We have read Item 4 of GenMark Diagnostics, Inc.’s Form 8-K dated June 27, 2013, and have the following comments:

1. We agree with the statements made in Item 4.01 (a).

2. We have no basis on which to agree or disagree with the statements made in Item 4.01 (b).

Yours truly,

/s/ Deloitte & Touche LLP