The Securities and Exchange Commission has not necessarily reviewed the information in this filing and has not determined if it is accurate and complete.
The reader should not assume that the information is accurate and complete.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 13F

FORM 13F COVER PAGE

OMB APPROVAL
OMB Number: 3235-0006
Estimated average burden
hours per response: 23.8

Report for the Calendar Year or Quarter Ended: 09-30-2016
Check here if Amendment    Amendment Number:
This Amendment (Check only one.):    is a restatement.
   adds new holdings entries.
Institutional Investment Manager Filing this Report:
Name: TWO SIGMA ADVISERS, LP
Address: 100 Avenue of the Americas
16th Floor
New York, NY  10013
Form 13F File Number: 028-14367

The institutional investment manager filing this report and the person by whom it is signed hereby represent that the person signing the report is authorized to submit it, that all information contained herein is true, correct and complete, and that it is understood that all required items, statements, schedules, lists, and tables, are considered integral parts of this form.

Person Signing this Report on Behalf of Reporting Manager:
Name: Matthew B. Siano, Esq.
Title: General Counsel
Phone: 212-625-5712
Signature, Place, and Date of Signing:
/s/ Matthew B. Siano New YorkNY 11-14-2016
[Signature] [City, State] [Date]
This Form 13F holdings report is being filed on behalf of Two Sigma Advisers, LP ("Advisers"). Advisers serves as the sole manager or investment manager, as applicable, of (i) Two Sigma U.S. All Cap Core Equity Fund, LP ("All Cap"), Two Sigma U.S. Small Cap Core Equity Fund, LP ("Small Cap"), Two Sigma Absolute Return Portfolio, LLC ("Absolute Return"), Two Sigma Active Extension U.S. All Cap Equity Portfolio, LLC ("Active Extension All Cap"), Two Sigma Active Extension U.S. Small Cap Equity Fund, LP ("Active Extension Small Cap"), Two Sigma U.S. Equity Variable Exposure Portfolio, LLC ("Equity Variable Exposure"), Two Sigma Constellation Portfolio, LLC ("Constellation"), Two Sigma Macro Portfolio, LLC ("Macro"), Two Sigma World Core Fund, LP ("World Core") and Two Sigma Equity Risk Premia Portfolio, LLC ("Equity Risk Premia"), ten investment entities that are managed by Advisers, each of which may be deemed to be an "other manager" for purposes of this Form 13F report, (ii) Two Sigma U.S. Large Cap Core Equity Portfolio, LLC ("Large Cap") and Two Sigma International Core Portfolio, LLC ("International Core"), two investment entities that are managed by Advisers, each of which may not presently be deemed to be an "other manager" for purposes of this Form 13F report and (iii) one or more separately managed accounts and subadvised accounts (collectively, the "Managed Accounts"), each of which may not presently be deemed to be an "other manager" for purposes of this 13F report. Advisers, rather than All Cap, Small Cap, Absolute Return, Active Extension All Cap, Active Extension Small Cap, Equity Variable Exposure, Constellation, Macro, World Core, Equity Risk Premia, Large Cap, International Core or the Managed Accounts, exercises sole investment discretion and voting authority over the securities held in the various accounts of All Cap, Small Cap, Absolute Return, Active Extension All Cap, Active Extension Small Cap, Equity Variable Exposure, Constellation, Macro, World Core, Equity Risk Premia, Large Cap, International Core and in the Managed Accounts.
Report Type (Check only one.):
X 13F HOLDINGS REPORT. (Check here if all holdings of this reporting manager are reported in this report.)
   13F NOTICE. (Check here if no holdings reported are in this report, and all holdings are reported by other reporting manager(s).)
   13F COMBINATION REPORT. (Check here if a portion of the holdings for this reporting manager are reported in this report and a portion are reported by other reporting manager(s).)
Form 13F Summary Page
Report Summary:
Number of Other Included Managers: 10
Form 13F Information Table Entry Total: 2,191
Form 13F Information Table Value Total: 23,477,249
(thousands)
List of Other Included Managers:
Provide a numbered list of the name(s) and Form 13F file number(s) of all institutional investment managers with respect to which this report is filed, other than the manager filing this report.
[If there are no entries in this list, state “NONE” and omit the column headings and list entries.]
No. Form 13F File Number Name
1 028-14405 Two Sigma U.S. All Cap Core Equity Fund, LP
2 028-14382 Two Sigma U.S. Small Cap Core Equity Fund, LP
3 028-15367 TWO SIGMA ABSOLUTE RETURN PORTFOLIO, LLC
4 028-15404 TWO SIGMA ACTIVE EXTENSION U.S. ALL CAP EQUITY PORTFOLIO, LLC
5 028-15364 Two Sigma Active Extension U.S. Small Cap Equity Fund, LP
6 028-15408 TWO SIGMA U.S. EQUITY VARIABLE EXPOSURE PORTFOLIO, LLC
7 028-15375 TWO SIGMA CONSTELLATION PORTFOLIO, LLC
8 028-16751 Two Sigma Macro Portfolio, LLC
9 028-17350 Two Sigma World Core Fund, LP
10 028-17346 Two Sigma Equity Risk Premia Portfolio, LLC