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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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 fZv0QLNO92Lc/G/oeIZI+A==

<SEC-DOCUMENT>0001477399-09-000004.txt : 20091124
<SEC-HEADER>0001477399-09-000004.hdr.sgml : 20091124
<ACCEPTANCE-DATETIME>20091124123241
ACCESSION NUMBER:		0001477399-09-000004
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20091124
DATE AS OF CHANGE:		20091124
EFFECTIVENESS DATE:		20091124

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			CSA Biotechnology Fund II, LLC
		CENTRAL INDEX KEY:			0001477399
		IRS NUMBER:				261358377
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-136347
		FILM NUMBER:		091203989

	BUSINESS ADDRESS:	
		STREET 1:		2501 TWENTIETH PLACE SOUTH
		STREET 2:		SUITE 275
		CITY:			BIRMINGHAM
		STATE:			AL
		ZIP:			35223
		BUSINESS PHONE:		2052632332

	MAIL ADDRESS:	
		STREET 1:		2501 TWENTIETH PLACE SOUTH
		STREET 2:		SUITE 275
		CITY:			BIRMINGHAM
		STATE:			AL
		ZIP:			35223
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001477399</cik>
        <entityName>CSA Biotechnology Fund II, LLC</entityName>
        <issuerAddress>
            <street1>2501 TWENTIETH PLACE SOUTH</street1>
            <street2>SUITE 275</street2>
            <city>BIRMINGHAM</city>
            <stateOrCountry>AL</stateOrCountry>
            <zipCode>35223</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>2052632332</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Liability Company</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2007</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Madding</firstName>
                <lastName>King III</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2501 Twentieth Place South</street1>
                <street2>Suite 275</street2>
                <city>Birmingham</city>
                <stateOrCountry>AL</stateOrCountry>
                <zipCode>35223</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Hollis</firstName>
                <middleName>N.</middleName>
                <lastName>Gieger</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2501 Twentieth Place South</street1>
                <street2>Suite 275</street2>
                <city>Birmingham</city>
                <stateOrCountry>AL</stateOrCountry>
                <zipCode>35223</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>J.</firstName>
                <middleName>Rainer</middleName>
                <lastName>Twiford</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2501 Twentieth Place South</street1>
                <street2>Suite 275</street2>
                <city>Birmingham</city>
                <stateOrCountry>AL</stateOrCountry>
                <zipCode>35223</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Investment Banking</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$5,000,001 - $25,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001477399-09-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2008-02-28</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isOtherType>true</isOtherType>
            <descriptionOfOtherType>LLC Units</descriptionOfOtherType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>25000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Brookline Investments, Inc.</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>Sterne Agee &amp; Leach, Inc.</associatedBDName>
                <associatedBDCRDNumber>791</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>800 Shades Creek Parkway</street1>
                    <street2>Suite 700</street2>
                    <city>Birmingham</city>
                    <stateOrCountry>AL</stateOrCountry>
                    <zipCode>35209</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>AL</state>
                    <state>CA</state>
                    <state>GA</state>
                    <state>MS</state>
                    <state>TN</state>
                    <state>TX</state>
                    <state>VA</state>
                    <state>WI</state>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>10000000</totalOfferingAmount>
            <totalAmountSold>8550000</totalAmountSold>
            <totalRemaining>1450000</totalRemaining>
            <clarificationOfResponse>This offering is closed.  263 units sold in Alabama, 10 units in California, 8 units in Georgia, 2 units in Mississippi, 4 units in Nevada, 17 units in Tennessee, 5 units in Texas, 5 units in Virginia, 8 units in Virgin Islands, 20 units in Wisconsin.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>58</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>CSA Biotechnology Fund II, LLC</issuerName>
                <signatureName>Matthew A. Aiken</signatureName>
                <nameOfSigner>Matthew A. Aiken</nameOfSigner>
                <signatureTitle>Authorized Company Signatory by Power of Attorney</signatureTitle>
                <signatureDate>2009-11-24</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
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