Exhibit 16.1

June 20, 2024

Securities and Exchange Commission

100 F Street, N.E.

Washington, DC 20549

Commissioners:

We have read the statements made by Esports Entertainment Group, Inc. under Item 4.01 of its Form 8-K dated June 13, 2024. We agree with the statements concerning our Firm in such Form 8-K; we are not in a position to agree or disagree with other statements of Esports Entertainment Group, Inc. contained therein.

Very truly yours,

/s/ Marcum llp

Marcum llp