Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the Registration Statements (No. 333-234235, No. 333-252078, No. 333-252903, No. 333-257433, No. 333-257661 and No. 333-250945) on Form S-3 and Registration Statements (No. 333-203703, No. 333-210369, No. 333-216858, No. 333-224062, No. 333-230041, No. 333-236794, No. 333-252743 and No. 333-257429) on Form S-8 of Zevra Therapeutics, Inc. (f/k/a KemPharm, Inc.) of our report dated March 30, 2022 relating to the financial statements of KemPharm, Inc. for the year ended December 31, 2021, appearing in this Annual Report on Form 10-K of Zevra Therapeutics, Inc. for the year ended December 31, 2022.

/s/ RSM US LLP

Orlando, Florida

March 7, 2023