Exhibit 25.1

_____________________________________________________________________________

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

__________________________

FORM T-1

STATEMENT OF ELIGIBILITY UNDER

THE TRUST INDENTURE ACT OF 1939 OF A

CORPORATION DESIGNATED TO ACT AS TRUSTEE

Check if an Application to Determine Eligibility of

a Trustee Pursuant to Section 305(b)(2)

_______________________________________________________

U.S. BANK NATIONAL ASSOCIATION

(Exact name of Trustee as specified in its charter)

31-0841368

I.R.S. Employer Identification No.

800 Nicollet Mall Minneapolis, Minnesota	55402
(Address of principal executive offices)	(Zip Code)

Monique L. Green

U.S. Bank National Association

1021 East Cary Street

Richmond, VA 23219

(804) 343-1566

(Name, address and telephone number of agent for service)

AGNC Investment Corp.

(Issuer with respect to the Securities)

Delaware	26-1701984
(State or other jurisdiction of incorporation or organization)	(I.R.S. Employer Identification No.)

2 Bethesda Metro Center, 12th Floor Bethesda. MD	20814
(Address of Principal Executive Offices)	(Zip Code)

Debt Securities

(Title of the Indenture Securities)

FORM T-1

Item 1.GENERAL INFORMATION. Furnish the following information as to the Trustee.

a)    Name and address of each examining or supervising authority to which it is subject.

            Comptroller of the Currency

            Washington, D.C.

        b)    Whether it is authorized to exercise corporate trust powers.

        Yes

Item 2. AFFILIATIONS WITH THE OBLIGOR. If the obligor is an affiliate of the Trustee, describe each such affiliation.

        None

Items 3-15    Items 3-15 are not applicable because to the best of the Trustee's knowledge, the obligor is not in default under any Indenture for which the Trustee acts as Trustee.

Item 16. LIST OF EXHIBITS: List below all exhibits filed as a part of this statement of eligibility and qualification.

1. A copy of the Articles of Association of the Trustee.*

    2. A copy of the certificate of authority of the Trustee to commence business, attached as Exhibit 2.

        3.    A copy of the certificate of authority of the Trustee to exercise corporate trust powers, attached as Exhibit 3.

        4.    A copy of the existing bylaws of the Trustee.**

    5.    A copy of each Indenture referred to in Item 4. Not applicable.

    6.    The consent of the Trustee required by Section 321(b) of the Trust Indenture Act of 1939, attached as Exhibit 6.

    7.    Report of Condition of the Trustee as of March 31, 2021 published pursuant to law or the requirements of its supervising or examining authority, attached as Exhibit 7.

    * Incorporated by reference to Exhibit 25.1 to Amendment No. 2 to registration statement on S-4, Registration Number 333-128217 filed on November 15, 2005.

    ** Incorporated by reference to Exhibit 25.1 to registration statement on form S-3ASR, Registration Number 333-199863 filed on November 5, 2014.

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SIGNATURE

    Pursuant to the requirements of the Trust Indenture Act of 1939, as amended, the Trustee, U.S. BANK NATIONAL ASSOCIATION, a national banking association organized and existing under the laws of the United States of America, has duly caused this statement of eligibility and qualification to be signed on its behalf by the undersigned, thereunto duly authorized, all in the Richmond, State of Virginia on the 2nd of June, 2021.

By:	/s/ Monique L. Green
Name:	Monique L. Green
Title:	Vice President

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Exhibit 2

4

Exhibit 3

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Exhibit 6

CONSENT

    In accordance with Section 321(b) of the Trust Indenture Act of 1939, the undersigned, U.S. BANK NATIONAL ASSOCIATION hereby consents that reports of examination of the undersigned by Federal, State, Territorial or District authorities may be furnished by such authorities to the Securities and Exchange Commission upon its request therefor.

Dated: June 2, 2021

By:	/s/ Monique L. Green
Name:	Monique L. Green
Title:	Vice President

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Exhibit 7

U.S. Bank National Association

Statement of Financial Condition

As of 3/31/2021

($000’s)

    3/31/2021

Assets

Cash and Balances Due From         $ 43,386,652

Depository Institutions

    Securities        154,609,348

    Federal Funds        0

    Loans & Lease Financing Receivables         297,075,286

    Fixed Assets         6,148,452

    Intangible Assets        13,371,986

    Other Assets         27,974,559

Total Assets         $542,566,283

Liabilities

Deposits        $444,618,948

    Fed Funds        1,321,015

    Treasury Demand Notes        0

    Trading Liabilities         1,231,176

    Other Borrowed Money        27,466,875

    Acceptances        0

    Subordinated Notes and Debentures        3,350,000

    Other Liabilities        13,255,984

Total Liabilities        $491,243,998

Equity

Common and Preferred Stock        18,200

    Surplus         14,266,915

    Undivided Profits        36,236,966

Minority Interest in Subsidiaries        800,204

Total Equity Capital        $51,322,285

Total Liabilities and Equity Capital        $542,566,283

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