CUSIP No. 67059M100 

Exhibit 1

JOINT FILING AGREEMENT

Joint Filing Agreement, dated as of February 14, 2011, is by and among Quaker BioVentures II, L.P., Quaker BioVentures Capital II, L.P., Quaker BioVentures Capital II, LLC, BioAdvance Ventures, L.P., BioAdvance GP I, L.P., and BioAdvance GP II, Inc. (the “Quaker Filers”).

Each of the Quaker Filers may be required to file with the United States Securities and Exchange Commission a statement on Schedule 13G with respect to shares of Common Stock, par value $0.001 per share, of Nupathe, Inc. beneficially owned by it from time to time. Pursuant to and in accordance with Rule 13(d)(1)(k) promulgated under the Securities Exchange Act of 1934, as amended, each of the Quaker Filers hereby agree to be responsible for the timely filing of the Schedule 13G and any amendments thereto on behalf of the Quaker Filers, and for the completeness and accuracy of the information concerning itself contained therein. Each of the Quaker Filers hereby further agree to file this Joint Filing Agreement as an exhibit to the statement and each such amendment, as required by such rule.

This Joint Filing Agreement may be terminated by any of the Quaker Filers upon one week’s prior written notice or such lesser period of notice as the Quaker Filers may mutually agree.

Executed and delivered as of the date first above written.

QUAKER BIOVENTURES II, L.P.
By:  Quaker BioVentures Capital II, L.P., its general partner
By:  Quaker BioVentures Capital II, LLC, its general partner
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President
QUAKER BIOVENTURES CAPITAL II, L.P.
By:  Quaker BioVentures Capital II, LLC, its general partner
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President
QUAKER BIOVENTURES CAPITAL II, LLC
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President

Page 14 of 15 Pages

CUSIP No. 67059M100 

BIOADVANCE VENTURES, L.P. By:  BioAdvance GP I, L.P., its general partner
By:  BioAdvance GP II, Inc., its general partner
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President
BIOADVANCE GP I, L.P.
By:  BioAdvance GP II, Inc., its general partner
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President
BIOADVANCE GP II, INC.
/s/ Richard S. Kollender
Name:	Richard S. Kollender
Title:	Vice President

Page 15 of 15 Pages