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Equity Incentive Plans and Warrants (Tables)
9 Months Ended
Sep. 30, 2023
Equity Incentive Plans and Warrants [Abstract]  
Stock Option Plan Activity
Activity under the Company’s stock option plans for the nine months ended September 30, 2023 was as follows:

   
Number of
options
   
Weighted
average
exercise
price
 
Outstanding at December 31, 2022     931,550     $ 3.97  
Granted     5,000       1.18  
Forfeited     (50,055 )     1.87  
Outstanding at March 31, 2023     886,495     $ 4.07  
Granted
    153,200       0.75  
Forfeited
    (20,024 )     3.94  
Outstanding at June 30, 2023
    1,019,671     $ 3.57  
Granted
    1,064,800       0.49  
Forfeited
    (5,200 )     1.25  
Outstanding at September 30, 2023     2,079,271     $ 2.00  
                 
Vested and exercisable at September 30, 2023     1,217,298     $ 2.62  
Estimated Using Assumptions
Options granted under our 2021 Stock Option Plan for the nine months ended September 30, 2023 to employees and nonemployees were 1,150,000 and 73,000, respectively and the weighted average exercise prices were $0.52 and $0.64, respectively. The weighted-average fair values of the options granted to employees and nonemployees were $0.42 and $0.51, respectively and were estimated using the following Black-Scholes assumptions:

    Employee
     Nonemployee  
Expected term (in years)
   
5.60
      5.49  
Risk‑free interest rate
   
4.06
%
    3.96 %
Dividend yield
   
%
    %
Expected volatility
   
105.14
%
    106.58 %
Share-based Compensation Expense
The following table shows the share-based compensation expense related to vested stock option grants to employees and nonemployees by financial statement line item on the accompanying statement of comprehensive loss:
 
    Three Months Ended September 30,         Nine Months Ended September 30,  
   
2023
   
2022
    2023     2022  
Research and development
 
$
79,561
     
10,820
      131,812       73,395  
Sales and marketing
   
623
     
1,515
      (1,319 )     3,857  
General and administrative
   
424,175
     
39,427
      496,036       81,036  
Total share-based compensation expense
 
$
504,359
     
51,762
      626,529       158,288  
Warrants Valuation Assumptions
The common stock was valued at $1,133,480, based on the Company’s stock price. The pre-funded warrants and common warrants were valued at $1,615,701 and $1,854,099, respectively, using the following Black-Scholes assumptions:

   
Pre-funded
warrants
   
Common
warrants
 
Expected term (in years)
   
4
     
4
 
Risk‑free interest rate
   
3.83
%
   
3.83
%
Dividend yield
   
%    
%
Expected volatility
   
100.25
%
   
100.25
%
Exercise price
 
$
0.0001
   
$
1.095
 
Stock price
 
$
0.86
   
$
0.86
 
Black-Scholes value
  $ 0.86     $ 0.58