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                          February 2, 2021

       John S. Kovach
       Chief Executive Officer
       Lixte Biotechnology Holdings, Inc.
       248 Route 25A, No. 2
       East Setauket, NY 11733

                                                        Re: Lixte Biotechnology
Holdings, Inc.
                                                            Registration
Statement on Form S-3
                                                            Filed January 26,
2021
                                                            File No. 333-252430

       Dear Mr. Kovach:

              This is to advise you that we have not reviewed and will not
review your registration
       statement.

               Please refer to Rules 460 and 461 regarding requests for
acceleration. We remind you
       that the company and its management are responsible for the accuracy and
adequacy of their
       disclosures, notwithstanding any review, comments, action or absence of
action by the staff.

                                                        Please contact Ada D.
Sarmento at 202-551-3798 with any questions.




                          Sincerely,


                          Division of Corporation Finance

                          Office of Life Sciences
       cc:                                              David Ficksman, Esq.