Certification of PEO and PFO of the Registrant pursuant to Section 906

/s/ KEVIN S. MCCARTHY

/s/ TERRY A. HART

Kevin S. McCarthy

Terry A. Hart

Chairman of the Board of Directors and Chief Executive Officer

Chief Financial Officer and Treasurer

EX-99.906 CERT 3 d420144dex99906cert.htm CERTIFICATION OF PEO AND PFO OF THE REGISTRANT PURSUANT TO SECTION 906 Certification of PEO and PFO of the Registrant pursuant to Section 906

EXHIBIT 99.906 CERT

SECTION 906 CERTIFICATIONS

Kevin S. McCarthy, Principal Executive Officer, and Terry A. Hart, Principal Financial Officer, of Kayne Anderson MLP Investment Company (the “Company”), each certify to his knowledge that:

The Company’s periodic report on Form N-CSR for the semi-annual period ended May 31, 2017 (the “Report”) fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934; and

The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.

July 27, 2017