Calculation of Filing Fee Tables

Form N-2

(Form Type)

Nuveen Municipal High Income Opportunity Fund

(Exact Name of Registrant as Specified in its Charter)

Table 1: Newly Registered and Carry Forward Securities

Security Type

Security Class Title

Fee Calculation or Carry Forward Rule

Amount Registered

Proposed Maximum Offering Price Per Unit

Maximum Aggregate Offering Price

Fee Rate

Amount of Registration Fee

Carry Forward Form Type

Carry Forward File Number

Carry Forward Initial effective date

Filing Fee Previously Paid In Connection with Unsold Securities to be Carried Forward

Newly Registered Securities (1)(2)

Fees to Be Paid

Equity

Common Shares $0.01 par value per share

Rule 456(b) and Rule 457(r)

Other

Rights to purchase Common Shares (3)

--

Equity

Preferred Shares

Rule 456(b) and Rule 457(r)

Unallocated (Universal) Shelf

N/A

Rule 456(b) and Rule 457(r)

Fees Previously Paid

Equity

Common Shares, $0.01 par value per share

--

--

--

--

--

--

Other

Rights to purchase Common Shares (3)

--

Equity

Preferred Shares

--

Unallocated (Universal) Shelf

N/A

--

Carry Forward Securities

Carry Forward Securities

Equity

Common Shares, $0.01 par value per share

415(a)(6)

20,766,793

$14.87

$308,802,212

0.0000927(4)

$28,625.97

N-2

333-253965

March 8, 2021

$28,625.97(5)

Total Offering Amounts

$308,802,217

--

Total Fees Previously Paid

$28,625.97

Total Fee Offsets

--

Net Fee Due

$0

(1) An indeterminate number of common shares, preferred shares and subscription rights to purchase common shares are being registered as may from time to time be offered, on an immediate, continuous or delayed basis, at indeterminate prices.

(2) In accordance with Rule 456(b) and Rule 457(r) under the Securities Act of 1933, as amended (the “Securities Act”), the Registrant is deferring payment of all of the registration fees and will pay any registration fees subsequently in advance or on a pay-as-you-go basis.

(3) No separate consideration will be received by the Registrant. Any shares issued pursuant to an offering of rights to purchase Common Shares, including any shares issued pursuant to an over-subscription privilege or a secondary over subscription privilege, will be shares registered under this Registration Statement.

(4) The then-current fee rate was $92.70 per $1,000,000.

(5) The Registrant previously registered 32,766,418 Common Shares in reliance on Rule 457 under the Securities Act, with respect to which the Registrant paid filing fees of $48,965, in prospectus supplements to its prior Registration Statement (File No. 333-253965), which went effective automatically on March 8, 2021 (the “Prior Registration Statement”). As of the time of this filing, 20,766,793 Common Shares remain unsold from the Prior Registration Statement. Pursuant to Rule 415(a)(6) under the Securities Act, this Registration Statement carries forward such unsold Common Shares, with respect to which $28,625.97 in filing fees have already been paid. Because this Registration Statement only includes such carry forward securities for which a registration fee was previously paid, no additional filing fees are currently due.