-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
 MFgwCgYEVQgBAQICAf8DSgAwRwJAW2sNKK9AVtBzYZmr6aGjlWyK3XmZv3dTINen
 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 FKfk67U0I1Wq0ervHIjsIA8KMjDCb/T8n3bMqx2+LKvVATIPjrWRbiaxtuhSE/FX
 HvUugSNkqn0L13ssQ8qJMQ==

<SEC-DOCUMENT>0001262463-09-000023.txt : 20090330
<SEC-HEADER>0001262463-09-000023.hdr.sgml : 20090330
<ACCEPTANCE-DATETIME>20090330193538
ACCESSION NUMBER:		0001262463-09-000023
CONFORMED SUBMISSION TYPE:	3
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20090317
FILED AS OF DATE:		20090330
DATE AS OF CHANGE:		20090330

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			BARNES MARYLEW
		CENTRAL INDEX KEY:			0001459355

	FILING VALUES:
		FORM TYPE:		3
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	333-68008
		FILM NUMBER:		09715645

	MAIL ADDRESS:	
		STREET 1:		23 LOCUST LANE
		CITY:			RYE
		STATE:			NY
		ZIP:			10580

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			NUVILEX, INC.
		CENTRAL INDEX KEY:			0001157075
		STANDARD INDUSTRIAL CLASSIFICATION:	AGRICULTURE CHEMICALS [2870]
		IRS NUMBER:				621772151
		STATE OF INCORPORATION:			NV
		FISCAL YEAR END:			0430

	BUSINESS ADDRESS:	
		STREET 1:		1971 OLD CUTHBERT ROAD
		CITY:			CHERRY HILL
		STATE:			NJ
		ZIP:			08034
		BUSINESS PHONE:		(856) 354-0707

	MAIL ADDRESS:	
		STREET 1:		1971 OLD CUTHBERT ROAD
		CITY:			CHERRY HILL
		STATE:			NJ
		ZIP:			08034

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	EFOODSAFETY COM INC
		DATE OF NAME CHANGE:	20010808
</SEC-HEADER>
<DOCUMENT>
<TYPE>3
<SEQUENCE>1
<FILENAME>primary_doc.xml
<DESCRIPTION>PRIMARY DOCUMENT
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0203</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2009-03-17</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001157075</issuerCik>
        <issuerName>NUVILEX, INC.</issuerName>
        <issuerTradingSymbol>NVLX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001459355</rptOwnerCik>
            <rptOwnerName>BARNES MARYLEW</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>23 LOCUST LANE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>RYE</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10580</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>CFO and Secretary</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock, par value $0.0001</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>121805</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <ownerSignature>
        <signatureName>Marylew Barnes</signatureName>
        <signatureDate>2009-03-30</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----