Yield10 Bioscience, Inc. (NASDAQCM:YTEN) Investor Presentation Yield10 is developing new technologies to achieve step-changes in crop yield to enhance global food security December 2017 Filed pursuant to Rule 433 Registration Statement No. 333-221283 December 11, 2017

 The statements made by Yield10 Bioscience, Inc. (the “Company,” “we,” “our” or “us”) herein regarding the Company and its business may be forward-looking in nature and are made pursuant to the safe harbor provisions of the Private Securities Litigation Reform Act of 1995. Forward-looking statements describe the Company’s future plans, projections, strategies and expectations, including statements regarding future results of operations and financial position, business strategy, prospective products and technologies, timing for receiving and reporting results of field tests and likelihood of success, and objectives of the Company for the future, and are based on certain assumptions and involve a number of risks and uncertainties, many of which are beyond the control of the Company, including, but not limited to, the risks detailed in the Company’s Annual Report on Form 10-k for the year ended December 31, 2016 and other reports filed by the Company with the Securities and Exchange Commission (the “SEC”). Forward-looking statements include all statements which are not historical facts, and can generally be identified by terms such as anticipates, believes, could, estimates, intends, may, plans, projects, should, will, would, or the negative of those terms and similar expressions. Because forward-looking statements are inherently subject to risks and uncertainties, some of which cannot be predicted or quantified and may be beyond the Company’s control, you should not rely on these statements as predictions of future events. Actual results could differ materially from those projected due to our history of losses, lack of market acceptance of our products and technologies, the complexity of technology development and relevant regulatory processes, market competition, changes in the local and national economies, and various other factors. All forward-looking statements contained herein speak only as of the date hereof, and the Company undertakes no obligation to update any forward-looking statements, whether to reflect new information, events or circumstances after the date hereof or otherwise, except as may be required by law. *Under the Private Securities Litigation Reform Act of 1995

This presentation highlights basic information about us and the offering. Because it is a summary that has been prepared solely for informational purposes, it does not contain all of the information that you should consider before investing in our company. Except as otherwise indicated, this presentation speaks only as of the date hereof. This presentation does not constitute an offer to sell, nor a solicitation of an offer to buy, any securities by any person in any jurisdiction in which it is unlawful for such person to make such an offering or solicitation. Neither the Securities and Exchange Commission (the “SEC”) nor any other regulatory body has approved or disapproved of our securities or passed upon the accuracy or adequacy of this presentation. Any representation to the contrary is a criminal offense. This presentation includes industry and market data that we obtained from industry publications and journals, third-party studies and surveys, internal company studies and surveys, and other publicly available information. Industry publications and surveys generally state that the information contained therein has been obtained from sources believed to be reliable. Although we believe the industry and market data to be reliable as of the date of this presentation, this information could prove to be inaccurate. Industry and market data could be wrong because of the method by which sources obtained their data and because information cannot always be verified with complete certainty due to the limits on the availability and reliability of raw data, the voluntary nature of the data gathering process and other limitations and uncertainties. In addition, we do not know all of the assumptions that were used in preparing the forecasts from the sources relied upon or cited herein. We have filed a Registration Statement on Form S-1 with the SEC, including a preliminary prospectus dated November 2, 2017 (the “Preliminary Prospectus”) and an amended Form S-1/A dated November 22, 2017, with respect to the offering of our securities to which this communication relates. Before you invest, you should read the Preliminary Prospectus (including the risk factors described therein) and, when available, the final prospectus relating to the offering, and the other documents filed with the SEC and incorporated by reference into the Preliminary Prospectus, for more complete information about us and the offering. You may obtain these documents, including the Preliminary Prospectus, for free by visiting EDGAR on the SEC website at http://sec.gov. Alternatively, we or any underwriter participating in the offering will arrange to send you the prospectus if you request it by contacting Ladenburg Thalmann & Co. Inc., 277 Park Avenue, 26th Floor, New York, NY 10172 or by email at prospectus@ladenburg.com.

 “Yield10 designs precise alterations to gene activity in food and feed crops to produce higher yields with lower inputs of land, water or fertilizer” Leverages a large historical investment in advanced metabolic engineering into the Ag space • 15 recent patent applications for increased crop yield Applying a technology approach/knowledge base that has been historically productive at a time when a critical new tool, genome editing, is available Has significant, near-term milestones in major row crops • Data from field tests of C3003 in Camelina, canola and soybean expected in 2018 Has numerous opportunities for value capture Has an organization structured to achieve upcoming milestones

 COMPANY OVERVIEW

 Yield10 Bioscience (Nasdaq:YTEN) is developing technologies to enhance global food security • Headquartered in Woburn, MA USA • Oilseeds center of excellence in Saskatoon, Canada Yield10 is bringing extensive expertise and track record in optimizing the flow of carbon in living systems to the agriculture sector to increase yield in key row crops • Yield10 is targeting step-change, or 10-20% increases, in seed yield • Our technology is based on 15 plus years of cutting edge crop metabolic engineering research • 15 recent patent applications for increased crop yield • Focus on major North American crops: canola, soybean and corn Yield10 will focus on its core strengths of advanced bioscience and innovation • Discover and develop proprietary crop yield technologies and de-risk them by developing proof points in canola, soybean and corn to optimize value capture from licensing or acquisition

[LOGO]

 Increasing Crop Yield is Valuable but Technically Very Challenging • First generation Ag biotech is based on using microbial genes in crops (early 90’s) • Over the last 20 years transgenic screening of thousands of single plant genes has failed • Development of modern corn from ancient teosinte provides insights into the path forward Teosinte (2-3 inch ear, 12 hard kernels) Domestication of Teosinte to modern corn ~9000 years of domestication Modification of 5-6 key genes Hybrid corn (12 inch ears, 500 kernels) • Genome editing enables precise modification of gene combinations in crops $50 billion1 question: what combination of corn genes can be modified to double modern corn yield? • Yield10 applies a powerful platform for identifying gene combinations to increase crop yield 1 2 x value of 2016 corn harvest, see slide 16

Increasing Crop Yield is a Multi-Gene Problem Road map A CO2 Metabolic map 2800 traffic lights Seed yield “roadmap analogy B Corn ~2400 transcription factor genes Seed Traffic regulators Road infrastructure Metabolic infrastructureGene regulators • Yield10: Two discovery platforms • “Smart Carbon Grid” optimizes crop metabolism or infrastructure • “T3 Platform” identifies key transcription factor genes • Yield10 is progressing a number of Performance Traits from these platforms in key crops • Unmet need: Smart technology for identifying key crop gene combinations •3.3 million experiments would be required to test all two gene combinations of transcription factors in corn • Yield10 is integrating its two platforms to create a “Google Earth” or “Waze”-like map of carbon flow (traffic) in crops

 SUMMARY OF OUR CROP YIELD TRAITS IN DEVELOPMENT Business Area Current Status Seed Yield Traits-Regulated C3003 Camelina 1st and 2nd generation at field testing stage Canola 1st generation at field testing stage Soybean and rice in development Seed/Oil Enhancing Traits-Non-Regulated C3004 Camelina testing underway C3007 Camelina, canola editing underway C3008a Camelina non-regulated1 status achieved C3008a, C3008b and C3009 combinations Camelina, editing of all 3 gene targets underway Additional oil trait combinations Research in progress Yield Improvement Discovery Platform C4001 Wheat program underway Rice transformation underway Corn transformation next step C4002 Corn transformation next step C4003 Wheat program underway Rice transformation underway Corn transformation next step C4004 Editing in rice underway C4004 plus 24 additional crop gene targets Research with rice and wheat next step Many opportunities exist for licensing and/or partnerships

 MARKET OPPORTUNITY

 Yield10 is…….Aligned with compelling megatrends 9 October 2009; Revised June, 2015 GA/EF/3242 Food Production Must Double by 2050 Global Food Security…… increasing overall demand and increased protein consumption Health and Wellness.....improved nutrition profile Food Safety and Sustainability…..growing interest in a “seed to plate value chain” Innovation…..new technology approaches, “big data/metabolic modeling” and genome editing

 Next Phase of High-Tech Crops, Editing Their Genes (May 7, 2017 By Jacob Bunge) •Genome editing completes the toolbox for enhancing crop yield and value •Genome editing enables Precision Molecular Breeding of gene combinations for enhancing crop yield •Genome edited plants may be non-regulated reducing product development timelines and costs1 • Regulated traits: Average 13 years and $130 M to develop • Non-regulated traits: Potential for 3-6 years, less than $10 M to develop •Licenses to CRISPR/Cas9 for crops may be readily available2 •The race is on to identify novel gene combinations for editing to improve crop performance 1 https://www.aphis.usda.gov/aphis/ourfocus/biotechnology/sa_brs_vpm/340-peis 2 https://broadinstitute.org/news/dupont-pioneer-and-broad-institute-join-forces-enabling

 Research license to C3003 and C3004 to improve yield in soybean • Market leader in GM soybean seed in the United States and North America • Non-exclusive term of approx. 3.5 years, preserves YTEN downstream rights • Develop and test C3003 in proprietary soybean lines • Develop and test C3003 combined with C3004 in proprietary soybean lines • Test traits in world class Monsanto soybean development program • Contributes significant resources and expertise in soybean research and breeding to YTEN C3003/C3004 yield trait program “The early development work with C3003 in oilseed plants and its mechanism is very interesting, and we are excited to have the opportunity to explore the potential of this unique yield trait gene in soybean. We are also impressed by Yield10’s metabolic engineering and advanced carbon flux modeling capabilities, as Monsanto is committed to developing solutions that meet farmers’ important needs, while positively affecting modern agriculture’s carbon footprint and overall sustainability.” Janice Edwards, Ph.D., Director, Yield Traits and Disease.

 Monsanto’s net sales for the seeds and genomics segment was $10.9 billion in FY 2017. • The seeds and genomics segment produces seed brands and develops biotechnology traits. MONSANTO’S SEEDS AND GENOMICS SEGMENT1 – FY 2011 - 2017 $12,000 $10,000 $695 $665 $523 $440 $615 $815 $ millions $6,000 $847 $895 $1,542 $779 $851 $1,771 $821 $867 $816 $801 $1,653 $2,102 $2,276 $2,162 $2,662 $4,000 $2,000 $4,805 $5,814 $6,596 $6,401 $5,953 $5,825 $6,270 $-2011 2012 2013 2014 2015 2016 2017 Corn seed and traits Soybean seed and traits Vegetable seeds Cotton seed and traits 1 Monsanto Company 2017 Form 10-K, page 25

Value Creation Model: Seed Yield and Oil Content Yield10’s gene traits may enable value creation through step-change increases in crop yield An illustrative example of the annual revenue opportunity for Yield10’s canola, soybean and corn gene traits based on the 2016 harvest. Canola(1) (Can) 2016 Harvest $18.4 M tons $9.6 B value 20% Target Yield Increase Soybean(2) (US) 2016 Harvest $4.36 B bu $40.1 B value 20% Target Yield Increase Corn(2) (US) 2016 Harvest $15.2 B bu $50.2 B value 10% Target Yield Increase For Soybean: Additional market opportunity emerging for High Oleic soybean oil. As genome editing traits deployed, a role for genome editing traits to boost oil biosynthesis (in range of 20%) could drive additional value for growers and Yield10. USDA projected on-farm corn price 2016-2017 is $3.30/bu USDA projected soybean price for 2016-2017 is $9.20/bu AAFC projected canola price 2016-2017 is $520/tonne +$1.92 B value 40-50% Value - Seed Co/Yield 10 $0.77 - $0.96 B value Yield10 Target3 $65 - $82 M potential revenue +$8.01 B value 40-50% Value - Seed Co/Yield 10 $3.2 - $4.0 B value Yield10 Target3 $272 - $340 M potential revenue +$5.16 B value 40-50% Value - Seed Co/Yield 10 $2.0 - $2.6 B value Yield10 Target3 $170 - $221 M potential revenue 1. http://www.statcan.gc.ca/daily-quotidien/161206/dq161206b-eng.htm 2. https://www.nass.usda.gov/Newsroom/2017/01_12_2017.php; High Plains/Midwest AG Journal, Jan. 19, 2017 3. Yield10 target of 5-12% of the value add for yield traits; used 8.5% in calculations 16

 Yield10 Traits: Market Doubleplay CropValue PropositionCompetitive Advantage Commodity • Corn • Soybean • Canola • Reduce grower cost • Increase production • Lower food costs • Food security • Sustainability • Novel yield traits • Powerful trait gene combination discovery platform High performance traits Specialty • Oilseed focus • Identity preserved • Health and wellness • Sustainability • Alternative crops • Industrial feedstocks • Novel yield traits • Increased oil content • Lower production costs

Commercial Strategy Yield10 Technologies Enable Multiple Paths to Value Creation Driven by Yield Traits and Unique Capabilities Major North American Commodity Crops Accelerate deployment with Ag majors Provide low hurdle to deploy and test yield traits in elite germplasm License agreements with milestones and participation in downstream economics Specialty and Niche Crops including Nutritional Oils Form collaborations based on combining technologies to improve yield and/or improve nutritional value Focus on development of new products in food and animal feed Utilize technologies enabling a non-regulated path to market JV-type agreements with significant share of downstream economics Yield10 Technology Platforms Accelerate innovation based on unique approach to identifying gene combinations for editing Access government grants and relationships with leading plant scientists R&D support for partner funded programs

 MILESTONES

 Yield10 is working to progress our yield enhancement technologies and build collaborations • Continue progress on C3003 with additional constructs and crops •Begin planning and logistics for 2018 field testing of C3003 in Camelina and canola in Canada •Work with Monsanto to set up testing program in soybean for 2018 •Report greenhouse data on C3003 trait in rice in 2018 and continue evaluating soybean • Progress oil enhancing targets using CRISPR genome editing •C3004, C3007, C3008 and C3009 for increased seed yield and seed oil content • Progress C4000 series traits into rice and corn •Report greenhouse data for C4003 in rice in 2018 •Begin work on C4000 series traits in corn •24 downstream transcription factors and combinations from the T3 Platform - genome editing target focus • Secure Ag industry collaborations and non-dilutive sources of funding • Build our intellectual property portfolio • Communicate our scientific innovations in technical presentations and papers

 Indicative Proof Point Timelines for C3003 Year 2017 2018 2019 Camelina/Gen 1 C3003  Field test data (Q1) Camelina/Gen 2 C3003  Greenhouse data (Q1)  Field test data (Q4) Field test data (Q4) Canola/Gen 1 C3003  Field test data (Q4) Field test data (Q4) Field trial Canola/Gen 2 C3003 Field test data (Q4)* Field trial Soybean/Gen 1 C3003  Preliminary Greenhouse data Field test TBD 1 Field test Soybean /Gen 2 C3003  Preliminary Greenhouse data Field test TBD 1 Field test Rice/Gen 1 C3003 Greenhouse data TBD *,1 Rice/Gen 2 C3003 Greenhouse data TBD *,1 * Progress depends on results achieved in greenhouse studies 1 Progress depends on seed bulk up in greenhouse

 Plans for 2018 Field Tests and Related Discovery & Early Development Activities Goal: Identify best constructs/events to move into larger scale field studies Camelina • Field tests with best lines with Gen 2.0 and Gen 2.1 constructs using field grown seed • Continue research on Gen 3 C3003 constructs Canola • Results from Camelina translate into canola for Gen C3003, repeat field tests with Gen1 canola using field grown seed • Scale up Gen 2.0 lines with intention to do field trials in 2019 at latest and in 2018 if technically possible • Develop Gen 2.1 lines of canola Soybean • Gen 1 and Gen 2 C3003 • Preliminary greenhouse indications: effects of C3003 translate into soybean • E.g. lower individual seed weight with soybean Gen 1, typical individual seed weight with Gen 2 • Preliminary results suggest an increase in branching for some events • Plan to conduct pilot scale field test with seed from greenhouse study (2018 or 2019) • Progress additional events for Gen 1, Gen 2.0 and develop Gen 2.1 lines of soybean • Support Monsanto soybean activity with C3003 and C3004

 TRAIT GENE DEVELOPMENT

Novel Yield Trait Gene: C3003 C3003 is a component of an algal system for increasing photosynthesis in low CO2 conditions • A scientific discovery from University of Massachusetts with a unique biological mechanism • C3003 improves the metabolic infrastructure of the plants • Potential to be useful in a wide range of C3 crops: Camelina, canola, soybean, corn, wheat, rice etc. Scientific progress provides new insights on mechanism • Two additional patent applications filed • Recent DOE grant sub-awardee 2017 research program for C3003 • Leverage the development speed of Camelina to optimize the impact of C3003 in major crops • Determine if Camelina results with C3003 translate into canola, soybean and rice A 5% reduction of photorespiration in soybean and wheat would add ~$500 million/year of economic value in the US (Walker et al., 2016, Ann. Rev. Plant Biol. 67:17.1 – 17.23)

 First Field Test with Gen 2.0 C3003 Shows Promising Results in Camelina Objectives • Generate technical data and determine the best way to deploy C3003 in canola and soybean Results • Solid agronomic profile: seed germination, seedling vigor • Overall seed yield results positive but varied between the two sites; the range was 2 to 7% (factors: weather conditions, site location, soil differences, nitrogen application) • Harvest index at maturity increased versus control plants in majority of events • Average individual seed weight1 increased above control plants in majority of events • Seed oil content and fatty acid profile unchanged Next Steps • Generate additional data on Gen 2.0 and 2.1 constructs and optimize seed specific promoter activity C3003 Camelina field test 2017 Low HIHigh HI Harvest index (HI) is a ratio of seed to plant biomass 25 1 determined by measuring mass of 1000 seeds

Gen 2 C3003 Camelina Mini Cage/Seed Bulk Up Results Mini Cage Results Provide Insight into Optimizing Expression of C3003 in Oilseed Crops Objectives and Growth Conditions • Produce pure field grown seed for 2018 field testing program • Compare 2 different seed specific promoters (Gen 2.0 and 2.1 constructs) Observations • Solid agronomic performance • Cage trials produced higher yields than control plants for most lines • Note: cage trials provide only an indication of performance • 6 out of 9 lines produced increase in seed yield versus control plants with range of seed yield • Gen 2.1 construct looks particularly promising Results strongly support deployment of Gen 2.0 and Gen 2.1 versions of C3003 in canola and soybean C3003 Camelina Mini Cages 2017 aSeed yield harvested per cage plot. Values are the average of 2 cages for all data points except * where only one cage was harvested

 First Field Test with Gen 1 C3003 Shows Translation of Yield Trait into Canola Objectives • Determine if seed yield improvement and agronomic attributes observed for C3003 in Camelina translate to canola • Test several events and select best ones for further study Results • Observed seed yield improvements of up to 13% in the best line versus control plants • Note: low rainfall conditions at site • Observed decrease in individual seed weight as seen with Gen 1 C3003 in Camelina Next Steps • Complete data analysis: seed oil content and fatty acid profile • Optimize seed specific promoter activity based on findings from work on Gen 2.0 and Gen 2.1 versions of C3003 in Camelina Key Finding: Gen 1 C3003 results in Camelina translate into canola C3003 canola field test Aug. 2017 Yield of harvested seed converted to kg/ha based on size of plot. Cont. = control wild-type plants

Yield10:Traits for Boosting Seed Oil Content USDA-APHIS confirmed nonregulated status for our genome-edited C3008a Camelina line • First nonregulated trait (C3008a) submission to USDA-APHIS by Yield10 • Developing multi-gene edited oilseed lines (C3008a, b, C3009) for future submission • C3007 and C3010 also accessible through genome editing to increase oil content C3007 a promising target for boosting oil content in oilseed crops • A scientific discovery from University of Missouri • A unique regulatory mechanism controlling oil biosynthesis • Potentially accessible through genome editing (reduce time to market?) • Combine C3007 with other genome edited traits to re-engineer oil biosynthesis

 • C4001 boosts a key parameter of photosynthesis (~75%) and improves plant biomass yield (~75-100%) in switchgrass • Transformed rice with C4001 gene from switchgrass and rice • 1st generation plants are growing in greenhouse • Produce 3rd generation plants and measure seed yield • Signed two-year collaboration with The National Research Council (NRC) of Canada to improve yield and drought tolerance in North American wheat • Focus on C4000 series of traits • NRC contributes financial resources and expertise in wheat research and breeding • Yield10 retains rights to IP • Corn transformation in planning stage C4001 in rice wheat

 KEY ACCOMPLISHMENTS & CAPITALIZATION TABLE

  Achieved all major objectives for 2017 while managing cash usage Kicked off 2017 with renaming and rebranding as Yield10 Bioscience, Inc. Added 2 key scientists and board member with agricultural business experience Conducted Field Tests for C3003 in Camelina and canola Reported encouraging results for C3003 in Camelina, canola and soybean Signed a research agreement with Monsanto for testing of C3003 and C3004 in soybean Reported results showing that trait C4001 produces significant increases in plant yield Signed a research collaboration with NRC to improve yield and drought tolerance in wheat Secured option from University of Missouri for C3007 technology to boost oil content Received confirmation of nonregulated status from USDA-APHIS for genome-edited Camelina Filed 5 patent applications Raised $2.0M, net in offering of common stock and warrants

 *Strike price for 393,300, 570,784, and 30,000 warrants is $39.80, $5.04, and $2.90, respectively.

 Yield10 Bioscience, Inc. (NASDAQCM:YTEN) Investor Presentation Yield10 is developing new technologies to achieve step-changes in crop yield to enhance global food security December 2017 Filed pursuant to Rule 433 Registration Statement No. 333-221283 December 11, 2017