Exhibit 22.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the following Registration Statements:

1. Registration Statement (Form S-3 No. 333-227398) of Chembio Diagnostics, Inc.,

2. Registration Statement (Form S-3 No. 333- 215813) of Chembio Diagnostics, Inc.,

3. Registration Statement (Form S-8 No. 333- 151785) pertaining to the 2008 Stock Incentive Plan of Chembio Diagnostics, Inc., and

4. Registration Statement (Form S-8 No. 333- 203633) pertaining to the 2014 Stock Incentive Plan and an employment agreement of Chembio Diagnostics, Inc.;

of our report dated March 11, 2021, with respect to the consolidated financial statements of Chembio Diagnostics, Inc, included in this Annual Report (Form 10-K) of Chembio Diagnostics, Inc. for the year ended December 31, 2020.

/s/ Ernst & Young, LLP

Jericho, New York

March 11, 2021