April 20, 2007

BY FACSIMILE (202) 772-9217 AND EDGAR

Mr. Jeffrey Riedler
Division of Corporation Finance
U.S. Securities and Exchange Commission
450 Fifth Street, N.W.
Washington, D.C. 20549-0405

Re: Chembio Diagnostics, Inc.
Post Effective Amendment No. 3 to Form SB-2 Registration Statement
Filed April 20, 2007
File Number 333-125942

Dear Mr. Riedler :

     Pursuant to Rule 461 of the General Rules and Regulations under the Securities Act of 1933, as amended, Chembio Diagnostics, Inc. (the “Company”) hereby requests that the effective date of the above-referenced Registration Statement be accelerated so that the Registration Statement may become effective at 8:00 a.m. Eastern Time on Wednesday, April 25, 2007, or at such later time as the Company may request by telephone to the Commission.

     The Company hereby confirms that it is aware of its obligations under the Securities Act and the Securities Exchange Act of 1934, as amended, with respect to the registration of securities specified in the above-referenced Registration Statement. Further, the Company acknowledges that:

• should the Commission or the staff, acting pursuant to delegated authority, declare the filing effective, it does not foreclose the Commission from taking any action with respect to the filing;

• the action of the Commission or the staff, acting pursuant to delegated authority, in declaring the filing effective, does not relieve the Company from its full responsibility for the adequacy and accuracy of the disclosure in the filing; and

• the Company may not assert the declaration of effectiveness as a defense in any proceeding initiated by the Commission or any person under the federal securities laws of the United States.

     If you or any member of the Staff has comments or questions, please contact our counsel at Patton Boggs, LLP, Alan Talensick at (303) 894-6378 or Richard Larkin at (631) 924-1135.

Very truly yours, CHEMBIO DIAGNOSTICS, INC.
By:	/s/ Richard J. Larkin
	Richard J. Larkin, Chief Financial Officer