Exhibit 25.1

_____________________________________________________________________________

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

__________________________

FORM T-1

Statement of Eligibility Under

The Trust Indenture Act of 1939 of a

Corporation Designated to Act as Trustee

Check if an Application to Determine Eligibility of

a Trustee Pursuant to Section 305(b)(2)

_______________________________________________________

U.S. BANK NATIONAL ASSOCIATION

(Exact name of Trustee as specified in its charter)

31-0841368

I.R.S. Employer Identification No.

800 Nicollet Mall Minneapolis, Minnesota	55,402
(Address of principal executive offices)	(Zip Code)

Wally Jones

U.S. Bank National Association

150 Fourth Avenue North, 2^nd Floor

Nashville, TN 37219

(615) 251-0733

(Name, address and telephone number of agent for service)

Symbion, Inc.

(Issuer with respect to the Securities)

Delaware	62-1625480
(State or other jurisdiction of incorporation or organization)	(I.R.S. Employer Identification No.)

40 Burton Hills Boulevard, Suite 500 Nashville, Tennessee	37,215
(Address of Principal Executive Offices)	(Zip Code)

8.00% SENIOR SECURED NOTES DUE 2016

(Title of the Indenture Securities)

Exhibit 25

FORM T-1

Item 1.GENERAL INFORMATION. Furnish the following information as to the Trustee.

a)Name and address of each examining or supervising authority to which it is subject.

Comptroller of the Currency

Washington, D.C.

b)Whether it is authorized to exercise corporate trust powers.

Yes

Item 2. AFFILIATIONS WITH OBLIGOR. If the obligor is an affiliate of the Trustee, describe each such affiliation.

None

Items 3-15 Items 3-15 are not applicable because to the best of the Trustee's knowledge, the obligor is not in default under any Indenture for which the Trustee acts as Trustee.

Item 16. LIST OF EXHIBITS: List below all exhibits filed as a part of this statement of eligibility and qualification.

1. A copy of the Articles of Association of the Trustee.*

2. A copy of the certificate of authority of the Trustee to commence business, attached as Exhibit 2.

3.A copy of the certificate of authority of the Trustee to exercise corporate trust powers, attached as Exhibit 3.

4.A copy of the existing bylaws of the Trustee.**

5.A copy of each Indenture referred to in Item 4. Not applicable.

6.The consent of the Trustee required by Section 321(b) of the Trust Indenture Act of 1939, attached as Exhibit 6.

7.Report of Condition of the Trustee as of June 30, 2011 published pursuant to law or the requirements of its supervising or examining authority, attached as Exhibit 7.

* Incorporated by reference to Exhibit 25.1 to Amendment No. 2 to registration statement on S-4, Registration Number 333-128217 filed on November 15, 2005.

** Incorporated by reference to Exhibit 25.1 to registration statement on S-4, Registration Number 333-166527 filed on May 5, 2010.

SIGNATURE

Pursuant to the requirements of the Trust Indenture Act of 1939, as amended, the Trustee, U.S. BANK NATIONAL ASSOCIATION, a national banking association organized and existing under the laws of the United States of America, has duly caused this statement of eligibility and qualification to be signed on its behalf by the undersigned, thereunto duly authorized, all in the City of Nashville, State of Tennessee on the 28th of September, 2011.

By:/s/ Wally Jones________Wally Jones

Vice President

Exhibit 2

Exhibit 3

Exhibit 6

CONSENT

In accordance with Section 321(b) of the Trust Indenture Act of 1939, the undersigned, U.S. BANK NATIONAL ASSOCIATION hereby consents that reports of examination of the undersigned by Federal, State, Territorial or District authorities may be furnished by such authorities to the Securities and Exchange Commission upon its request therefor.

Dated: September 28, 2011

By:/s/ Wally Jones_________Wally Jones

Vice President

Exhibit 7

U.S. Bank National Association

Statement of Financial Condition

As of 6/30/2011

($000's)

		6/30/2011
Assets
	Cash and Balances Due From Depository Institutions	$	15,249,371
	Securities	63,952,096
	Federal Funds	15,876
	Loans & Lease Financing Receivables	190,017,874
	Fixed Assets	5,231,718
	Intangible Assets	13,050,819
	Other Assets	22,581,835
	Total Assets	310,099,589
Liabilities
	Deposits	$	218,820,466
	Fed Funds	7,695,079
	Treasury Demand Notes	—
	Trading Liabilities	550,498
	Other Borrowed Money	33,124,842
	Acceptances	—
	Subordinated Notes and Debentures	7,679,246
	Other Liabilities	8,693,748
	Total Liabilities	276,563,879
Equity
	Minority Interest in Subsidiaries	1,821,732
	Common and Preferred Stock	18,200
	Surplus	14,136,872
	Undivided Profits	17,558,906
	Total Equity Capital	33,535,710
Total Liabilities and Equity Capital		310,099,589